Expression and biological functions of Ancylostoma ceylanicum saposin-like protein

He, Long; Abuzeid, Asmaa M.I.; Zhuang, Tingting; Zhao, Qi; Zhu, Shilan; Chen, Xiaoyu; Liu, Jumei; Li, Xiu; Li, Guoqing

doi:10.1007/s00436-021-07313-6

Cited by 3 publications

(1 citation statement)

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“…We used a Lymphocyte Isolation Kit (TBD, Tianjin, China) to isolate canine PBMCs from a 2-ml sample of anticoagulated blood collected from a healthy dog, as described previously [ 12 ]. After lymphocyte growth was stabilized, the experimental groups (with purified protein), blank control, and negative control (BSA) were prepared.…”

Section: Methodsmentioning

confidence: 99%

In vitro immunoregulatory role of recombinant Ancylostoma ceylanicum calreticulin

Zhuang,

Abuzeid,

Chen

et al. 2024

Parasites Hosts Dis

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Ancylostoma ceylanicum is a zoonotic soil-derived nematode that parasitizes the intestines of humans and animals (dogs and cats), leading to malnutrition and iron-deficiency anemia. Helminth parasites secrete calreticulin (CRT), which regulates or blocks the host’s immune response. However, no data on A. ceylanicum calreticulin (Ace-CRT) are available. We investigated the biological function of recombinant Ace-CRT (rAce-CRT). rAce-CRT showed reliable antigenicity and stimulated the proliferation of mouse splenocytes and canine peripheral blood mononuclear cells. Quantitative reverse-transcription PCR assays revealed that rAce-CRT primarily promoted the expression of T helper 2 cytokines, particularly IL-13, in canine peripheral blood lymphocytes. rAce-CRT inhibited complement-mediated sheep erythrocyte hemolysis in vitro. Our findings indicate that Ace-CRT plays an immunomodulatory role and may be a promising candidate molecule for a hookworm vaccine.

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Section: Methodsmentioning

confidence: 99%