Expression and clinical significance of HSP27 and its phosphorylation in lower extremity arteriosclerosis obliterans

Bai, Junqing; Wang, Fule; Wang, Xuguang; Mutu, Eerde; Chen, Duan; Qi, Yili; Hu, Li-qiang; Gao, Zhan-feng

doi:10.7717/peerj.9305

Cited by 3 publications

(5 citation statements)

References 15 publications

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“…However, it is an invasive examination, and the contrast agent has renal toxicity, so it cannot be repeated for many times. Hence, it cannot be used as a way of postoperative review [ 15 , 16 ].…”

Section: Discussionmentioning

confidence: 99%

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Ultrasonic Image Feature Analysis under Deep Learning Algorithm to Evaluate the Efficacy of Drug-Coated Balloon for Treatment of Arteriosclerotic Occlusion

Zhang

Chen

et al. 2022

Computational and Mathematical Methods in Medicine

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In order to explore the adoption of ultrasonic images under deep learning (DL) algorithm to evaluate the efficacy of drug-coated balloon (DCB) for treatment of arteriosclerotic occlusion, 56 patients who underwent DCB surgery of lower limb artery were selected and all the patients received the examinations of algorithmic ultrasound and digital subtraction angiography (DSA) before surgery. According to the examination methods, they were classified into algorithmic ultrasound group and DSA group. One to two months after DCB surgery, ultrasound examination was performed with the region-based faster convolutional neural network (faster R-CNN) target detection algorithm to check the therapeutic effect. The results showed that the image effect processed by the target detection algorithm based on DL was signally better than that of traditional ultrasonic processing algorithm in Dice, precision (Pre), and sensitivity, with significant difference ( P < 0.05 ). Compared with DSA, algorithmic ultrasound showed better consistency between the two groups in the diagnosis of common femoral artery, superficial femoral artery, and popliteal artery stenosis, with statistical significance ( P < 0.05 ). However, for the diagnosis of anterior tibial artery stenosis, the consistency between algorithmic ultrasound and DSA was general. The residual stenosis of each artery segment decreased obviously in postoperative review compared with that before surgery, and the difference was statistically significant ( P < 0.05 ). Besides, both the pulsatility index (PI) and the blood flow velocity of the dorsalis pedis artery increased after surgery, compared with those before surgery, with significant differences ( P < 0.05 ). To sum up, ultrasound based on DL target detection algorithm had good imaging effect and good consistency with DSA, which was of the clinical reference value. Additionally, DCB surgery was helpful to treat arteriosclerosis occlusion and improve limb blood supply, which had clinical adoption value.

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Section: Discussionmentioning

confidence: 99%

“…The precision (Pre), Dice coefficient, and sensitivity [ 16 ] were used to evaluate. Equations ( 11 ), ( 12 ), and ( 13 ) show the calculations of these three indexes.…”

Section: Methodsmentioning

confidence: 99%

Ultrasonic Image Feature Analysis under Deep Learning Algorithm to Evaluate the Efficacy of Drug-Coated Balloon for Treatment of Arteriosclerotic Occlusion

Zhang

Chen

et al. 2022

Computational and Mathematical Methods in Medicine

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“…Circulating HSP27 was positively correlated with carotid intima-media thickness, an independent predictor of early diabetic atherosclerotic lesions, and may represent a novel marker of subclinical atherosclerosis in type 2 diabetes (101). Serum HSP27 and its phosphorylation levels were lower in patients with lower extremity occlusive atherosclerosis and negatively correlated with disease severity (102). Low serum HSP27 levels were independently associated with the occurrence of CVD and sudden cardiac death in patients undergoing dialysis as well as with carotid atherosclerosis and oxidative stress.…”

Section: The Therapeutic Intervention Of Hsp27 41 Atherosclerosismentioning

confidence: 92%

Mechanistic insights into heat shock protein 27, a potential therapeutic target for cardiovascular diseases

Zou

Shi

Liu

et al. 2023

Front. Cardiovasc. Med.

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Heat shock protein 27 (HSP27) is a small chaperone protein that is overexpressed in a variety of cellular stress states. It is involved in regulating proteostasis and protecting cells from multiple sources of stress injury by stabilizing protein conformation and promoting the refolding of misfolded proteins. Previous studies have confirmed that HSP27 is involved in the development of cardiovascular diseases and plays an important regulatory role in this process. Herein, we comprehensively and systematically summarize the involvement of HSP27 and its phosphorylated form in pathophysiological processes, including oxidative stress, inflammatory responses, and apoptosis, and further explore the potential mechanisms and possible roles of HSP27 in the diagnosis and treatment of cardiovascular diseases. Targeting HSP27 is a promising future strategy for the treatment of cardiovascular diseases.

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“…Lower extremity arteriosclerosis obliterans (LEASO) is a manifestation of systemic atherosclerosis in the limbs. The growth of atherosclerotic material and secondary thrombosis cause the thickening of the intima of lower extremity arteries and the narrowing or even occlusion of the lumen and further cause a series of symptoms and signs of the affected limb, such as intermittent claudication, resting pain, ulcer necrosis, and even limb loss (1). Notable progress has been made in the treatment of LEASO, including surgical techniques, endovascular interventions, and pharmacological treatments.…”

Section: Introductionmentioning

confidence: 99%

“…Although studies have focused on the endothelial dysfunction in LEASO, chronic immune stimulation plays an important role in LEASO as it is derived and developed from atherosclerosis (1,3,4). In the plaque, low-density lipoprotein cholesterol is modified by oxidative processes [oxidized low-density lipoprotein (ox-LDL)] and taken up by macrophages.…”

Section: Introductionmentioning

confidence: 99%

Increased Level of Tim-3+PD-1+CD4+T Cells With Altered Function Might Be Associated With Lower Extremity Arteriosclerosis Obliterans

et al. 2022

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Lower extremity arteriosclerosis obliterans (LEASO) is a vascular disease that may result in adult limb loss worldwide. CD4+T cell-mediated immunity plays a significant role in LEASO. The T cell immunoglobulin and mucin domain 3 (Tim-3) and inhibitory receptor programmed cell death-1 (PD-1) are well-known immune checkpoints that play crucial roles in regulating CD4+T cell activation or tolerance. In this study, blood mononuclear cells were isolated from the blood samples of healthy controls and patients who were diagnosed with LEASO for the first time [stage III or IV according to the Fontaine classification system and had not received drugs (except for heparin) or surgery treatment]. We concluded the higher proportion of Tim-3+PD-1+CD4+T cells in human higher stage LEASO, and oxidized low-density lipoprotein increased Tim-3 and PD-1 co-expression by activating CD4+T cells in a dose- dependent manner. Tim-3+PD-1+CD4+T cells displayed a more active status and produced more anti-atherogenic cytokines compared to Tim-3-PD-1-CD4+T cells. Apart from the increased frequency, the altered function of Tim-3+PD-1+CD4+T cells was also observed in LEASO compared to those from healthy controls. These in vitro results indicated that Tim-3 and PD-1 might be promising early warning targets of higher stage LEASO. In addition, the blockade of Tim-3 and PD-1 signaling pathways aggravated the pro-atherogenic Th1 responses in LEASO, further suggesting that the cardiovascular safety must be a criterion considered in using immune checkpoint inhibitors to reverse T cell exhaustion during tumors and chronic viral infections.

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Expression and clinical significance of HSP27 and its phosphorylation in lower extremity arteriosclerosis obliterans

Cited by 3 publications

References 15 publications

Ultrasonic Image Feature Analysis under Deep Learning Algorithm to Evaluate the Efficacy of Drug-Coated Balloon for Treatment of Arteriosclerotic Occlusion

Ultrasonic Image Feature Analysis under Deep Learning Algorithm to Evaluate the Efficacy of Drug-Coated Balloon for Treatment of Arteriosclerotic Occlusion

Mechanistic insights into heat shock protein 27, a potential therapeutic target for cardiovascular diseases

Increased Level of Tim-3+PD-1+CD4+T Cells With Altered Function Might Be Associated With Lower Extremity Arteriosclerosis Obliterans

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