Expression and clinical significance of long non-coding RNA HNF1A-AS1 in human gastric cancer

Abstract

BackgroundIncreasing evidence has demonstrated that long non-coding RNAs (lncRNAs) play essential roles in the occurrence and development of human cancers, including gastric cancer (GC). However, the functional and clinical significance of lncRNAs are still poorly understood.MethodsIn this study, the expression of LncRNA HNF1A antisense RNA 1 (HNF1A-AS1) was first examined by lncRNAs microarray analysis in 6 GC tissues, and was then further verified by real-time quantitative reverse transcription PCR (qRT-PCR) both in 3 GC cell lines and 161 cases of GC tissues. We also evaluated the association between HNF1A-AS1 expression and clinicopathological features of patients with GC.ResultsLncRNAs microarray analysis results exhibited that HNF1A-AS1 was downregulated in GCs tissues (mean fold change 2.06, p < 0.05), which was further confirmed by qRT-PCR. The results from qRT-PCR showed that the expression of HNF1A-AS1 was not only downregulated in three GC cell lines (AGS, BGC-823, and MKN-45) relative to that in a normal gastric mucosal epithelial cell line (GES-1), but also decreased in GC tissues relative to that in paired adjacent non-neoplastic tissues (low expression, 94 of 161; low expression rate, 58.38 %). Furthermore, low HNF1A-AS1 expression was associated with tumor size/diameter (p = 0.005, multivariate analysis), levels of serum carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA19-9), and RRM1 expression in tissue samples (p = 0.028, p = 0.009, and p = 0.006, respectively).ConclusionsTaken together, our data indicate that lncRNA HNF1A-AS1 may be a regulator of GC, and thus, it may have potential as a novel biomarker and treatment target for this type of cancer.

HNF1A-AS1 promotes growth and metastasis of esophageal squamous cell carcinoma by sponging miR-214 to upregulate the expression of SOX-4

“…In a previous investigation, HAS1 was overexpressed in hepatocellular carcinoma (HCC) tissues and cell lines and the expression of HAS1 was closely related to tumor growth and tumor differentiation (48). In contrast, low expression of HAS1 was associated with tumor size in human gastric cancer (49). Additionally, HAS1 was upregulated in bladder cancer and high level HAS1 predicted bladder cancer metastasis (50).…”

The HNF1α-Regulated LncRNA HNF1α-AS1 Is Involved in the Regulation of Cytochrome P450 Expression in Human Liver Tissues and Huh7 Cells

“…The physiologic functions of HNF1a-AS1 were first identified in the regulation of cell proliferation and migration in esophageal adenocarcinoma cells . The role of HNF1a-AS1 in the promotion of cancer progression and metastasis in gastrointestinal tract organs has been reported, including in pancreatic cancer (Muller et al, 2015), gastric cancer (Dang et al, 2015), and hepatocellular carcinoma (Liu et al, 2016). HNF1a-AS1 has been considered an oncogene Fig.…”

Long non-coding RNA ERICH3-AS1 is an unfavorable prognostic factor for gastric cancer

“…Compared with the protein-coding gene, lncRNA has obvious developmental stagespecificity and tissue specificity (Hu et al, 2015). The function and mechanism of lncRNA are diverse; more importantly, lncRNA can regulate the expressions of protein-coding genes from multiple levels and multiple perspectives (Dang et al, 2015;Ding et al, 2015).…”