Expression and function of macrophage-inducible C-type lectin (Mincle) in inflammation driven parturition in fetal membranes and myometrium

Lim, Ratana; Lappas, Martha

doi:10.1111/cei.13281

Cited by 12 publications

(6 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Decreases in TLR signaling by the removal of their key partners or decreasing receptor numbers have also been studied. The elimination of Mincle, a sensor for lipids, was shown to lead to decreased effect of TLR ligands to cause inflammation in uterus (Lim and Lappas, 2019). In addition, GIT2 knockdown, in myometrial and amnion cells also lead to decreased inflammation in response to TLR ligands (Lim and Lappas, 2019).…”

Section: Damp/pamp Prr Pathway Manipulation: Potential Therapeutic Strategies For Fetal Membrane Maintenancementioning

confidence: 99%

“…The elimination of Mincle, a sensor for lipids, was shown to lead to decreased effect of TLR ligands to cause inflammation in uterus (Lim and Lappas, 2019). In addition, GIT2 knockdown, in myometrial and amnion cells also lead to decreased inflammation in response to TLR ligands (Lim and Lappas, 2019). The P2X7 receptor, which has been shown to regulate IL-1β release from gestational tissues working in concert with TLRs has also been studied.…”

Section: Damp/pamp Prr Pathway Manipulation: Potential Therapeutic Strategies For Fetal Membrane Maintenancementioning

confidence: 99%

See 1 more Smart Citation

The Role of Danger Associated Molecular Patterns in Human Fetal Membrane Weakening

2020

View full text Add to dashboard Cite

The idea that cellular stress (including that precipitated by stretch), plays a significant role in the mechanisms initiating parturition, has gained considerable traction over the last decade. One key consequence of this cellular stress is the increased production of Danger Associated Molecular Patterns (DAMPs). This diverse family of molecules are known to initiate inflammation through their interaction with Pattern Recognition Receptors (PRRs) including, Toll-like receptors (TLRs). TLRs are the key innate immune system surveillance receptors that detect Pathogen Associated Molecular Patterns (PAMPs) during bacterial and viral infection. This is also seen during Chorioamnionitis. The activation of TLR commonly results in the activation of the pro-inflammatory transcription factor Nuclear Factor Kappa-B (NF-kB) and the downstream production of pro-inflammatory cytokines. It is thought that in the human fetal membranes both DAMPs and PAMPs are able, perhaps via their interaction with PRRs and the induction of their downstream inflammatory cascades, to lead to both tissue remodeling and weakening. Due to the high incidence of infection-driven Pre-Term Birth (PTB), including those that have preterm Premature Rupture of the Membranes (pPROM), the role of TLR in fetal membranes with Chorioamnionitis has been the subject of considerable study. Most of the work in this field has focused on the effect of PAMPs on whole pieces of fetal membrane and the resultant inflammatory cascade. This is important to understand, in order to develop novel prevention, detection, and therapeutic approaches, which aim to reduce the high number of mothers suffering from infection driven PTB, including those with pPROM. Studying the role of sterile inflammation driven by these endogenous ligands (DAMPs) activating PRRs system in the mesenchymal and epithelial cells in the amnion is important. These cells are key for the maintenance of the integrity and strength of the human fetal membranes. This review aims to (1) summarize the knowledge to date pertinent to the role of DAMPs and PRRs in fetal membrane weakening and (2) discuss the clinical potential brought by a better understanding of these pathways by pathway manipulation strategies.

show abstract

Section: Damp/pamp Prr Pathway Manipulation: Potential Therapeutic Strategies For Fetal Membrane Maintenancementioning

confidence: 99%

Section: Damp/pamp Prr Pathway Manipulation: Potential Therapeutic Strategies For Fetal Membrane Maintenancementioning

confidence: 99%

The Role of Danger Associated Molecular Patterns in Human Fetal Membrane Weakening

2020

View full text Add to dashboard Cite

show abstract

“…Finally, functions of CTLs in intrauterine immunity and maternal–fetal tolerance [ 142 ], as well as in parturition [ 143 ], were shown in humans. Initial studies suggest a possible involvement of CTLs in veterinary species in these processes.…”

Section: Discussionmentioning

confidence: 99%

C-Type Lectins in Veterinary Species: Recent Advancements and Applications

Lindenwald

Lepenies

2020

IJMS

View full text Add to dashboard Cite

C-type lectins (CTLs), a superfamily of glycan-binding receptors, play a pivotal role in the host defense against pathogens and the maintenance of immune homeostasis of higher animals and humans. CTLs in innate immunity serve as pattern recognition receptors and often bind to glycan structures in damage- and pathogen-associated molecular patterns. While CTLs are found throughout the whole animal kingdom, their ligand specificities and downstream signaling have mainly been studied in humans and in model organisms such as mice. In this review, recent advancements in CTL research in veterinary species as well as potential applications of CTL targeting in veterinary medicine are outlined.

show abstract

“…Myometrial cells (Lim et al, 2013a), decidual cells (Araujo et al, 2018) and mesenchymal cells (Lim and Lappas, 2019) were isolated and cultured as previously described. Decidual tissue was collected from the chorion layer of fetal membranes.…”

Section: Primary Cell Culturementioning

confidence: 99%

Punicalagin Suppresses Mediators Involved in Labor Onset and Progression in vitro

Nguyen-Ngo¹,

Willcox²,

Lappas³

2021

American Journal of Immunology

View full text Add to dashboard Cite

Spontaneous preterm birth is the leading cause of neonatal morbidity and mortality. Inflammation plays a central role in the activation of myometrial contractions and rupture of fetal membranes associated with spontaneous preterm birth. The pomegranate polyphenol punicalagin is known to possess anti-inflammatory properties. In this study, we aimed to determine the effect of punicalagin on pro-inflammatory and pro-labor mediators in an in vitro model of intrauterine inflammation associated with preterm birth. Primary human cells isolated from myometrium, decidua and fetal membranes (amnion mesenchyme) were stimulated with or without IL1B or TNF, in the absence or presence of punicalagin. Punicalagin blocked inflammation-induced expression of pro-inflammatory cytokines, chemokines and extracellular matrix degrading enzymes in primary myometrial, decidual and amnion cells. Punicalagin also suppressed inflammation-induced myometrial activation as evidenced by a decrease in PTGS2 mRNA expression, PGF2α secretion and myometrial cell contractility in situ. Overall, punicalagin may block pro-inflammatory and pro-labor mediators in human gestational tissues. Further study should identify if punicalagin can delay LPS-induced spontaneous preterm birth in an in vivo mouse model.

show abstract

Expression and function of macrophage-inducible C-type lectin (Mincle) in inflammation driven parturition in fetal membranes and myometrium

Cited by 12 publications

References 61 publications

The Role of Danger Associated Molecular Patterns in Human Fetal Membrane Weakening

The Role of Danger Associated Molecular Patterns in Human Fetal Membrane Weakening

C-Type Lectins in Veterinary Species: Recent Advancements and Applications

Punicalagin Suppresses Mediators Involved in Labor Onset and Progression in vitro

Contact Info

Product

Resources

About