Expression and Function of the Testis-Predominant Protein LYAR in Mice

Lee, Boyeon; Jin, Sora; Choi, Heejin; Kwon, Jun Tae; Kim, Jihye; Jeong, Ji Won; Kwon, Younggoo; Cho, Chunghee

doi:10.1007/s10059-013-2271-3

Cited by 11 publications

(9 citation statements)

References 21 publications

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“…The interactive program depends on the extrinsic program, which reflects hormonal regulation of the somatic cells by testosterone and follicle-stimulating hormone. 3 4 These regulatory processes provide a comprehensive network of genes controlling male reproduction. In particular, the intrinsic program operates gene expression patterns for germ cell progression in a time-dependent manner.…”

Section: Introductionmentioning

confidence: 99%

Characterization of MAGEG2 with testis-specific expression in mice

et al. 2017

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Male germ cell development is a well-defined process occurring in numerous seminiferous tubules of the testis. Uncovering testicular novel genes related to intrinsic regulation of spermatogenesis is essential for the understanding of spermatogenesis. In the present study, we investigated mouse Mageg2, which belongs to a group of melanoma-associated antigens (MAGEs). Mageg2 is transcribed in the testis specifically, and its expression level is increased at the pachytene spermatocyte stage, indicating that Mageg2 is expressed predominantly in germ cells. We generated an antibody against mouse MAGEG2 for further characterization at the protein level. Immunoblot analysis suggested that MAGEG2 has specific testicular expression and the expression primarily occurred in pachytene spermatocytes. Proteomic analyses demonstrated that mouse MAGEG2 binded to testicular germ cell-specific serine/threonine-protein kinase 31 (STK31) and heat shock protein 9 (HSPA9). Direct binding with both interaction partners was confirmed by co-immunoprecipitation. We found that STK31 and HSPA9 bind MAGEG2 directly but not with each other. Interestingly, MAGEG2 reduced the kinase activity of STK31. Our study suggests that mouse MAGEG2 has at least two functions, including chaperone activity related to HSPA9 and regulation of pachytene spermatocyte-specific kinase, STK31. Altogether, our results provide the first information about MAGEG2 at the transcript and protein levels and suggest its potential molecular functions.

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Section: Introductionmentioning

confidence: 99%

Characterization of MAGEG2 with testis-specific expression in mice

et al. 2017

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“…BioMed Research International C2HC zinc finger motif and three nuclear localization signals. Lee et al [41] found that the LYAR protein was present in spermatocytes and spermatids, but not in sperm. However, we detected LYAR expression in sperm, and its expression decreased in sperm of obese rats and increased in ZnSO 4treated groups.…”

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confidence: 99%

The Mechanism of Zinc Sulfate in Improving Fertility in Obese Rats Analyzed by Sperm Proteomic Analysis

Ma¹,

Han²,

et al. 2020

BioMed Research International

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This study investigates the mechanism underlying the improving effect of zinc on fertility in obese rats using proteomics. The effects of three different doses of ZnSO4 on spermatogenesis and hormone levels were studied. Testicular spermatogenesis was observed by HE staining. Serum estrogen and testosterone levels were measured by chemiluminescent microparticle immunoassay. Sperm proteomic analysis was performed by liquid chromatography-mass spectrometry. The DAVID database was used to perform the GO enrichment analysis and KEGG pathway analysis of the differentially expressed genes, and the STRING online database was used to construct a PPI network. The sperm count, sperm motility, and testosterone hormones of the ZnSO4-treated rats group were increased. ZnSO4 improved testicular structure and spermatogenesis abnormalities caused by obesity. Proteomic analysis showed that there were 401 differentially expressed proteins in a total of 6 sperm samples from the ZnSO4-treated group and the obesity groups. Differential proteins were input into the DAVID website. The 341 identified proteins were then classified according to their biological functions. The KEGG analysis showed that the enriched signal pathways included glycolysis/gluconeogenesis, carbon metabolism, citrate cycle, fatty acid metabolism, and pyruvate metabolism. Some proteins were shown to be associated with valine, leucine, and isoleucine degradation pathways. STRING analysis obtained 36 node proteins. Cytoscape analysis showed that these proteins mainly participated in nine networks including metabolic process, oxidation-reduction, aerobic respiration, RNA splicing, and glutathione conjugation. ZnSO4 may improve the fertility of obese male rats by regulating protein expression related to metabolism, inflammation, and sperm maturation.

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“…Given that immature spermatocytes lack cell proliferation capacity, Lyar may have additional cellular function(s) other than regulating cell growth. Interestingly, Lyar‐deficient mice were fully fertile and showed intact spermatogenesis [Lee et al, ].…”

Section: Discussionmentioning

confidence: 99%

Lyar Is a New Ligand for Retinal Pigment Epithelial Phagocytosis

Guo

Ding

Caberoy

et al. 2015

J of Cellular Biochemistry

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Phagocytosis is critical to tissue homeostasis, as highlighted by phagocytosis defect of retinal pigment epithelial (RPE) cells with debris accumulation, photoreceptor degeneration and blindness. Phagocytosis ligands are the key to delineating molecular mechanisms and functional roles of phagocytes, but are traditionally identified in individual cases with technical challenges. We recently developed open reading frame phage display (OPD) for phagocytosis-based functional cloning (PFC) to identify unknown ligands. One of the identified ligands was Ly-1 antibody reactive clone (Lyar) with functions poorly defined. Herein, we characterized Lyar as a new ligand to stimulate RPE phagocytosis. In contrast to its reported nucleolar expression, immunohistochemistry showed that Lyar was highly expressed in photoreceptor outer segments (POSs) of the retina. Cytoplasmic Lyar was released from apoptotic cells, and selectively bound to shed POSs and apoptotic cells, but not healthy cells. POS vesicles engulfed through Lyar-dependent pathway were targeted to phagosomes and colocalized with phagosome marker Rab7. These results suggest that Lyar is a genuine RPE phagocytosis ligand, which in turn supports the validity of OPD/PFC as the only available approach for unbiased identification of phagocytosis ligands with broad applicability to various phagocytes.

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Expression and Function of the Testis-Predominant Protein LYAR in Mice

Cited by 11 publications

References 21 publications

Characterization of MAGEG2 with testis-specific expression in mice

Characterization of MAGEG2 with testis-specific expression in mice

The Mechanism of Zinc Sulfate in Improving Fertility in Obese Rats Analyzed by Sperm Proteomic Analysis

Lyar Is a New Ligand for Retinal Pigment Epithelial Phagocytosis

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