Expression and functional analysis of a novel Fusion Competent Myoblast specific GAL4 driver

Beckett, Karen; Rochlin, Kate; Duan, Hong; Nguyen, Hanh T.; Baylies, Mary K.

doi:10.1016/j.modgep.2007.10.002

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…To investigate which activities, besides JAK/STAT signaling, are required in the muscles for the immune responses against wasp infection, we individually suppressed several well-known immune-related signaling pathways, including the p38, c-Jun N-terminal kinase (JNK), Toll, and insulin signaling pathways. We suppressed these pathways by expressing either RNA interference or dominant-negative constructs with a muscle-specific driver 34 , Mef2-GAL4 , and then assayed encapsulation rates and hemocyte numbers. We found that suppression of insulin signaling in the muscles, by expressing InR RNAi or InR DN , significantly reduced the encapsulation rate (Fig.…”

Section: Resultsmentioning

confidence: 99%

Drosophila muscles regulate the immune response against wasp infection via carbohydrate metabolism

Yang

Hultmark

2017

Sci Rep

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We recently found that JAK/STAT signaling in skeletal muscles is important for the immune response of Drosophila larvae against wasp infection, but it was not clear how muscles could affect the immune response. Here we show that insulin signaling is required in muscles, but not in fat body or hemocytes, during larval development for an efficient encapsulation response and for the formation of lamellocytes. This effect requires TOR signaling. We show that muscle tissue affects the immune response by acting as a master regulator of carbohydrate metabolism in the infected animal, via JAK/STAT and insulin signaling in the muscles, and that there is indirect positive feedback between JAK/STAT and insulin signaling in the muscles. Specifically, stimulation of JAK/STAT signaling in the muscles can rescue the deficient immune response when insulin signaling is suppressed. Our results shed new light on the interaction between metabolism, immunity, and tissue communication.

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Section: Resultsmentioning

confidence: 99%

Drosophila muscles regulate the immune response against wasp infection via carbohydrate metabolism

Yang

Hultmark

2017

Sci Rep

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“…To directly investigate the role of muscle JAK/STAT signaling in the immune response, we suppressed individual components of this pathway in larval somatic muscles by expressing dominant‐negative constructs of either the cytokine receptor gene domeless ( dome DN ) or the single Drosophila STAT gene Stat92E ( Stat92E DN ), with the Mef2‐GAL4 muscle‐specific driver . This driver is expressed in larval muscles, but not for instance in fat body (Appendix Fig S3A, C and D).…”

Section: Resultsmentioning

confidence: 99%

JAK/STATsignaling inDrosophilamuscles controls the cellular immune response against parasitoid infection

et al. 2015

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The role of JAK/STAT signaling in the cellular immune response of Drosophila is not well understood. Here, we show that parasitoid wasp infection activates JAK/STAT signaling in somatic muscles of the Drosophila larva, triggered by secretion of the cytokines Upd2 and Upd3 from circulating hemocytes. Deletion of upd2 or upd3, but not the related os (upd1) gene, reduced the cellular immune response, and suppression of the JAK/STAT pathway in muscle cells reduced the encapsulation of wasp eggs and the number of circulating lamellocyte effector cells. These results suggest that JAK/STAT signaling in muscles participates in a systemic immune defense against wasp infection.

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“…The myoblast fusion is asymmetric and takes place between FCs and FCMs. Previous reports (Estrada et al, 2006; Beckett and Baylies, 2007; Beckett et al, 2008) were at the origin of a hypothesis that FCMs are not “naïve” myoblasts and contribute to the modulation of fusion process. In contrast, our results support a view that FCs rather than FCMs carry the instructive information and allow us to conclude that FCMs do not play an active role in setting the number of fusion events.…”

Section: Discussionmentioning

confidence: 99%

Downstream of Identity Genes: Muscle-Type-Specific Regulation of the Fusion Process

et al. 2010

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In all metazoan organisms, the diversification of cell types involves determination of cell fates and subsequent execution of specific differentiation programs. During Drosophila myogenesis, identity genes specify the fates of founder myoblasts, from which derive all individual larval muscles. Here, to understand how cell fate information residing within founders is translated during differentiation, we focus on three identity genes, eve, lb, and slou, and how they control the size of individual muscles by regulating the number of fusion events. They achieve this by setting expression levels of Mp20, Pax, and mspo, three genes that regulate actin dynamics and cell adhesion and, as we show here, modulate the fusion process in a muscle-specific manner. Thus, these data show how the identity information implemented by transcription factors is translated via target genes into cell-type-specific programs of differentiation.

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Expression and functional analysis of a novel Fusion Competent Myoblast specific GAL4 driver

Cited by 7 publications

References 21 publications

Drosophila muscles regulate the immune response against wasp infection via carbohydrate metabolism

Drosophila muscles regulate the immune response against wasp infection via carbohydrate metabolism

JAK/STATsignaling inDrosophilamuscles controls the cellular immune response against parasitoid infection

Downstream of Identity Genes: Muscle-Type-Specific Regulation of the Fusion Process

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