Expression and functional characterization of chemokine CXCL10 from Nile tilapia (Oreochromis niloticus) in response to bacterial infection and C1q-mediated regulation of monocytes/macrophages activity

Chen, Meng; Yan, Fangfang; Wei, Xiayi; Liu, Shuo; Han, Biao; Lin, Li; Ye, Jianmin

doi:10.1016/j.aqrep.2021.100778

Cited by 4 publications

(1 citation statement)

References 46 publications

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“…Structurally, PoCXCL10 possesses the four cysteine residues conserved in mammalian CXC, which form two intramolecular disulfide bonds that stabilize the molecule ( 54 ). Of the few reported fish CXCL10, they were shown to exhibit high levels of mRNA expression in immune relevant organs such as spleen, head kidney, and liver, and their expression levels could change significantly after bacterial challenge ( 35 , 37 , 38 ). Similarly, we found that PoCXCL10 expression was relatively high in liver and spleen, and was upregulated during the infection of E. tarda , V. anguillarum , and V. harveyi .…”

Section: Discussionmentioning

confidence: 99%

A Teleost CXCL10 Is Both an Immunoregulator and an Antimicrobial

Sun

2022

Front. Immunol.

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Chemokines are a group of cytokines that play important roles in cell migration, inflammation, and immune defense. In this study, we identified a CXC chemokine, CXCL10, from Japanese flounder Paralichthys olivaceus (named PoCXCL10) and investigated its immune function. Structurally, PoCXCL10 possesses an N-terminal coil, three β-strands, and a C-terminal α-helix with cationic and amphipathic properties. PoCXCL10 expression occurred in multiple tissues and was upregulated by bacterial pathogens. Recombinant PoCXCL10 (rPoCXCL10) promoted the migration, cytokine expression, and phagocytosis of flounder peripheral blood leukocytes (PBLs). rPoCXCL10 bound to and inhibited the growth of a variety of common Gram-negative and Gram-positive fish pathogens. rPoCXCL10 killed the pathogens by causing bacterial membrane permeabilization and structure destruction. When introduced in vivo, rPoCXCL10 significantly inhibited bacterial dissemination in fish tissues. A peptide derived from the C-terminal α-helix exhibited bactericidal activity and competed with rPoCXCL10 for bacterial binding. Deletion of the α-helix affected the in vitro bactericidal activity but not the chemotaxis or in vivo antimicrobial activity of PoCXCL10. Together, these results indicate that PoCXCL10 exerts the role of both an immunoregulator and a bactericide/bacteriostatic via different structural domains. These findings provide new insights into the immune function and working mechanism of fish CXC chemokines.

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Section: Discussionmentioning

confidence: 99%

A Teleost CXCL10 Is Both an Immunoregulator and an Antimicrobial

Sun

2022

Front. Immunol.

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Assessing the effect of β-glucan diets on innate immune response of tilapia macrophages against trichlorfon exposure: an in vitro study

Fierro-Castro,

Tort,

Mingatto

et al. 2023

Fish Physiol Biochem

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The widespread use of pesticides in some areas where fish species such as tilapia are farmed may cause damage to the environment and affect commercial fish and therefore, human health. Water leaching with the pesticide trichlorfon, during the fumigation season in the field, can affect water quality in fish farms and consequently affect fish health. At the same time, the use of immunomodulatory compounds such as β-glucan supplied in the diet has become widespread in fish farms as it has been shown that improves the overall immune response. The present research examines the immunomodulatory impacts observed in macrophages of Nile tilapia (Oreochromis niloticus) after being fed a diet supplemented with β-glucan for 15 days, followed by their in vitro exposure to trichlorfon, an organophosphate pesticide, at concentrations of 100 and 500 µg mL−1 for 24 h. The results showed that β-glucan diet improved the viability of cells exposed to trichlorfon and their antioxidant capacity. However, β-glucan did not counteract the effects of the pesticide as for the ability to protect against bacterial infection. From the present results, it can be concluded that β-glucan feeding exerted a protective role against oxidative damage in cells, but it was not enough to reduce the deleterious effects of trichlorfon on the microbicidal capacity of macrophages exposed to this pesticide.

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Advances in chemokines of teleost fish species

Liu

2024

Aquaculture and Fisheries

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Expression and functional characterization of chemokine CXCL10 from Nile tilapia (Oreochromis niloticus) in response to bacterial infection and C1q-mediated regulation of monocytes/macrophages activity

Cited by 4 publications

References 46 publications

A Teleost CXCL10 Is Both an Immunoregulator and an Antimicrobial

A Teleost CXCL10 Is Both an Immunoregulator and an Antimicrobial

Assessing the effect of β-glucan diets on innate immune response of tilapia macrophages against trichlorfon exposure: an in vitro study

Advances in chemokines of teleost fish species

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