Expression and Localization of SWAP-70 in Human Fetomaternal Interface and Placenta During Tubal Pregnancy and Normal Placentation

Liu, Jing; Dong, Li; Cao, Bin; Li, Yuxia; Piao, Yun-shang; Wang, Yanling

doi:10.1369/jhc.6a7151.2007

Cited by 6 publications

(4 citation statements)

References 43 publications

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“…Piezo type mechanosensitive ion channel component 1 ( PIEZO1 ), is an important molecular marker of blood flow in the placenta and has the potential to be a new therapeutic target for the treatment of placental vascular diseases [105]. Switching B cell complex subunit SWAP70 is involved in regulating migration and invasion of trophoblast cells during the processes of embryonic implantation as well as placentation [106]. Podocalyxin ( PODXL ) is significantly increased in early-onset preeclampsia and may represent a novel marker of maternal endothelial cell dysfunction [107,108].…”

Section: Discussionmentioning

confidence: 99%

Placenta Transcriptome Profiling in Intrauterine Growth Restriction (IUGR)

Majewska

Lipka

Paukszto

et al. 2019

IJMS

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Intrauterine growth restriction (IUGR) is a serious pathological complication associated with compromised fetal development during pregnancy. The aim of the study was to broaden knowledge about the transcriptomic complexity of the human placenta by identifying genes potentially involved in IUGR pathophysiology. RNA-Seq data were used to profile protein-coding genes, detect alternative splicing events (AS), single nucleotide variant (SNV) calling, and RNA editing sites prediction in IUGR-affected placental transcriptome. The applied methodology enabled detection of 37,501 transcriptionally active regions and the selection of 28 differentially-expressed genes (DEGs), among them 10 were upregulated and 18 downregulated in IUGR-affected placentas. Functional enrichment annotation indicated that most of the DEGs were implicated in the processes of inflammation and immune disorders related to IUGR and preeclampsia. Additionally, we revealed that some genes (S100A13, GPR126, CTRP1, and TFPI) involved in the alternation of splicing events were mainly implicated in angiogenic-related processes. Significant SNVs were overlapped with 6533 transcripts and assigned to 2386 coding sequence (CDS), 1528 introns, 345 5’ untranslated region (UTR), 1260 3’UTR, 918 non-coding RNA (ncRNA), and 10 intergenic regions. Within CDS regions, 543 missense substitutions with functional effects were recognized. Two known mutations (rs4575, synonymous; rs3817, on the downstream region) were detected within the range of AS and DEG candidates: PA28β and PINLYP, respectively. Novel genes that are dysregulated in IUGR were detected in the current research. Investigating genes underlying the IUGR is crucial for identification of mechanisms regulating placental development during a complicated pregnancy.

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Section: Discussionmentioning

confidence: 99%

Placenta Transcriptome Profiling in Intrauterine Growth Restriction (IUGR)

Majewska

Lipka

Paukszto

et al. 2019

IJMS

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“…Subsequently, its expression has been demonstrated in MC (14), primary embryonic fibroblasts (15), DC (16), trophoblasts (17) and gliomas (18). In B cells, SWAP-70 is involved not only in nuclear events but also in signaling events in the cytoplasm during cell activation (19) and in efficient homing to the lymph nodes (20).…”

Section: Introductionmentioning

confidence: 99%

Regulation of Eosinophil Trafficking by SWAP-70 and Its Role in Allergic Airway Inflammation

Bahaie

Hosseinkhani

et al. 2012

The Journal of Immunology

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Eosinophils are the predominant inflammatory cells recruited to allergic airways. Here we demonstrate that human and murine eosinophils express SWAP-70, an intracellular RAC-binding signaling protein, and examine its role in mediating eosinophil trafficking and pulmonary recruitment in a murine model of allergic airway inflammation. Compared to WT eosinophils, SWAP-70 deficient (Swap-70−/−) eosinophils revealed altered adhesive interactions within inflamed post capillary venules under conditions of blood flow by intravital microscopy exhibiting enhanced slow rolling but decreased firm adhesion. In static adhesion assays, Swap-70−/− eosinophils adhered poorly to VCAM-1 and ICAM-1 and exhibited inefficient leading edge and uropod formation. Adherent Swap-70−/− eosinophils failed to translocate RAC1 to leading edges and displayed aberrant cell surface localization/distribution of α4 and Mac-1. Chemokine-induced migration of Swap-70−/− eosinophils was significantly decreased correlating with reduced intracellular calcium levels, defective actin polymerization/depolymerization and altered cytoskeletal rearrangement. In vivo, compared to WT mice, recruitment of eosinophils to the lungs of allergen-challenged Swap-70−/− mice was significantly reduced along with considerable attenuation of airway inflammation indicated by diminished IL-5, IL-13 and TNFα levels, reduced mucus secretion and improved airway function. These findings suggest that regulation of eosinophil trafficking and migration by SWAP-70 is important for the development of eosinophilic inflammation after allergen exposure.

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“…Some DEGs were not located on the X-chromosome but were associated with placentation. For example, PLAU has been associated with trophoblast invasion [ 23 ] and SWAP70 has been associated with placentation [ 24 ]. We performed analyses of up-regulated and down-regulated genes from the comparison of male and female placenta samples.…”

Section: Resultsmentioning

confidence: 99%

Placental Transcriptome Profiling in Subtypes of Diabetic Pregnancies Is Strongly Confounded by Fetal Sex

Kedziora

Obermayer

Sugulle

et al. 2022

IJMS

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The placenta is a temporary organ with a unique structure and function to ensure healthy fetal development. Placental dysfunction is involved in pre-eclampsia (PE), fetal growth restriction, preterm birth, and gestational diabetes mellitus (GDM). A diabetic state affects maternal and fetal health and may lead to functional alterations of placental metabolism, inflammation, hypoxia, and weight, amplifying the fetal stress. The placental molecular adaptations to the diabetic environment and the adaptive spatio–temporal consequences to elevated glucose or insulin are largely unknown (2). We aimed to identify gene expression signatures related to the diabetic placental pathology of placentas from women with diabetes mellitus. Human placenta samples (n = 77) consisting of healthy controls, women with either gestational diabetes mellitus (GDM), type 1 or type 2 diabetes, and women with GDM, type 1 or type 2 diabetes and superimposed PE were collected. Interestingly, gene expression differences quantified by total RNA sequencing were mainly driven by fetal sex rather than clinical diagnosis. Association of the principal components with a full set of clinical patient data identified fetal sex as the single main explanatory variable. Accordingly, placentas complicated by type 1 and type 2 diabetes showed only few differentially expressed genes, while possible effects of GDM and diabetic pregnancy complicated by PE were not identifiable in this cohort. We conclude that fetal sex has a prominent effect on the placental transcriptome, dominating and confounding gene expression signatures resulting from diabetes mellitus in settings of well-controlled diabetic disease. Our results support the notion of placenta as a sexual dimorphic organ.

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Expression and Localization of SWAP-70 in Human Fetomaternal Interface and Placenta During Tubal Pregnancy and Normal Placentation

Cited by 6 publications

References 43 publications

Placenta Transcriptome Profiling in Intrauterine Growth Restriction (IUGR)

Placenta Transcriptome Profiling in Intrauterine Growth Restriction (IUGR)

Regulation of Eosinophil Trafficking by SWAP-70 and Its Role in Allergic Airway Inflammation

Placental Transcriptome Profiling in Subtypes of Diabetic Pregnancies Is Strongly Confounded by Fetal Sex

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