Expression and prognostic value of cell-cycle-associated genes in gastric adenocarcinoma

Abstract

BackgroundGastric carcinoma is a malignant disease, and gastric adenocarcinoma (GAC) is the most common histological type. Molecular profiling of GAC has been extensively performed, but few have focused on the clinical significance of gene clusters of the cell cycle.MethodsWe investigated the genetic profile of cell-cycle-associated genes in a GAC cohort. The mRNA expression and clinical data were downloaded from TCGA, according to cBioportal. We conducted a series of analyses to detect the relationships between these genes and GAC.ResultsFrom all the patients, 5 clusters were identified based on mRNA expression of 122 cell-cycle-associated genes. Cluster 1 showed the worst prognosis and is characterized by extremely high expression of WEE2 and CCNE1. Comparison of the gene patterns showed that 16 genes expressed were distinctly varied between each cluster. In addition, investigations into the prognostic role of the 16 genes suggested that high expression of ESPL1 and MCM5 were significantly correlated with favorable outcomes. Moreover, we detected that ESPL1 and MCM5 gene expression were negatively correlated with GAC pathologic stage progression.ConclusionsThis study revealed a gene expression pattern of the cell cycle in different GAC subgroups, and suggested individual genes were associated with the clinical outcome and AJCC stages. These results suggest a novel prognostic strategy for GAC and provide information for patient stratification and trials of targeted therapies.Electronic supplementary materialThe online version of this article (10.1186/s12876-018-0811-1) contains supplementary material, which is available to authorized users.

Mutational landscape of gastric cancer and clinical application of genomic profiling based on target next-generation sequencing

“…Amplifications mainly targeted well-known oncogenes such as EGFR , ERBB2 , KRAS , MYC , CCNE1 , JAK2 , FGFR2 et al 12 gastric tumor tissues harbored CCNE1 amplifications (7.84%), which was the most frequent genes with SCNA in this study, and with similar frequency (10.58%) in TCGA cohort [4]. Amplification of CCNE1 is associated with poor outcome in gastric [23], breast [24], and ovarian cancer [25, 26]. ERBB2 amplification was detected in 8 gastric tumor tissues (5.23%).…”

Identification of Potential Crucial Genes Associated With the Pathogenesis and Prognosis of Endometrial Cancer

“…Nevertheless, it has also been reported that ESPL1 plays an opposite role in gastric adenocarcinoma. Wang D. et al (2018) showed that ESPL1 levels were lower in gastric adenocarcinoma tissue in comparison with that of adjacent non-cancer tissue and was associated with longer overall survival and a low tumor stage suggesting the dual role of this gene in cancer. ESPL1 expression was found to be increased in our study, however, the clinical significance and functional mechanism of ESPL1 in EC remains to be verified.…”

Identification and Analysis of Genes Associated with Epithelial Ovarian Cancer by Integrated Bioinformatics Methods

“…Nevertheless, it has also been reported that ESPL1 plays an opposite role in gastric adenocarcinoma. ESPL1 expression was negatively correlated with gastric adenocarcinoma pathologic stage progression, and the high expression of ESPL1 was signi cantly correlated with favorable outcomes [35]. Further work is required to resolve the con icting roles of ESPL1 in cancer and determine its functions in cancers including the ovarian cancer.…”