2008
DOI: 10.1267/ahc.08018
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Expression and Trafficking of the .GAMMA. Subunit of Na,K-ATPase in Hypertonically Challenged IMCD3 Cells

Abstract: The γ subunit (FXYD2) of Na,K-ATPase is an important regulator of the sodium pump. In this investigation we have analysed the trafficking of γ to the plasma membrane in cultures of inner medullary collecting duct cells (IMCD3) following acute hypertonic challenge and brefeldin A (BFA) treatment. Following hypertonic challenging for 24 hr immunofluorescence labeling revealed initial co-localization of the γ subunit and 58K Golgi protein in the cytoplasm, but no co-localization of α1 and Golgi protein. Exposure … Show more

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Cited by 7 publications
(4 citation statements)
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“…Conversely, no obvious changes in the ␣1 subunit trafficking was observed between the c⌬7-and mock-transfected cells, suggesting no retention of ␣1 and ␤1 subunits by the truncated form of FXYD2b in intracellular compartments. Thus, the data confirmed the previous observations that association of the ␣␤ complexes with the FXYD protein (here, FXYD2b) occurs at the plasma membrane but not earlier in ER membranes (42). Immunocytochemistry supported the results obtained by cellular fractionation.…”
Section: Mislocalization Of Truncated Fxyd2bsupporting
confidence: 90%
“…Conversely, no obvious changes in the ␣1 subunit trafficking was observed between the c⌬7-and mock-transfected cells, suggesting no retention of ␣1 and ␤1 subunits by the truncated form of FXYD2b in intracellular compartments. Thus, the data confirmed the previous observations that association of the ␣␤ complexes with the FXYD protein (here, FXYD2b) occurs at the plasma membrane but not earlier in ER membranes (42). Immunocytochemistry supported the results obtained by cellular fractionation.…”
Section: Mislocalization Of Truncated Fxyd2bsupporting
confidence: 90%
“…Based on crystal structures of Na,K-ATPase, the FXYD subunit is positioned on the periphery of the complex (Toyoshima et al, 2011 ), which may be a structural basis for “easy” exchange of regulatory subunits. Association of FXYD proteins with alpha/beta complex occurs post-translationally either in Golgi or even at the plasma membrane (Crambert et al, 2002 ; Pihakaski-Maunsbach et al, 2008 ; Moshitzky et al, 2012 ). This may facilitate exchange of regulatory subunits within an already-functioning complex.…”
Section: Regulatory Subunits Of Nak-atpasementioning
confidence: 99%
“…Transcriptional changes may still occur, although being masked by the stable mRNA levels, as increased translation may lead to a decreased mRNA half-life and simultaneously increased transcription rate. Additionally, FXYD (and Na ϩ -K ϩ -ATPase) units may be trafficked from cytoplasmic vesicular pools to the plasma membrane (23,39).…”
Section: Expression Changes Of Fxyd Isoforms and Na ϩ -K ϩ -Atpasementioning
confidence: 99%