Expression Changes of PPARGC1A During the Development of Lean and Obese Pigs

Jiang, An-An; Li, Mingzhou; Zhang, Kai; Gu, Yiren; Li, Xuewei

doi:10.3923/javaa.2011.597.600

Cited by 3 publications

(3 citation statements)

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“…Thus, the composition of GI tract microbiota of obese and lean pigs could reflect that of corresponding human phenotypes. Erhualian and Landrace breeds are typically obese and lean pigs [ 11 – 14 ], respectively, thus their energy metabolism might be distinctive. Erhualian pig, a sister breed to Meishan (both belonging to Taihu pig), is a local porcine breed mainly located around the Taihu Lake area of China and is characterized by increased fat storage, tasty meat quality and high fertility [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs

Luo

Wright

et al. 2012

Archaea

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The diversity of fecal methanogens of Erhualian (obese type) and Landrace (lean type) pigs was examined using separate 16S rRNA gene libraries for each breed. A total of 763 clones were analyzed; 381 from the Erhualian library and 382 from the Landrace library were identified belonging to the genus Methanobrevibacter. Others were identified belonging to the genus Methanosphaera. The two libraries showed significant differences in diversity (P<0.05) and composition (P<0.0001). Only two operational taxonomic units (OTUs) were found in both libraries, whereas six OTUs were found only in the Erhualian library and 23 OTUs were found only in the Landrace library. Real-time PCR showed that the abundance of fecal methanogens in Landrace pigs was significantly higher than that in Erhualian pigs (P<0.05). Results showed that the Landrace pig (lean) harbored a greater diversity and higher numbers of methanogenmcrAgene copies than the Erhualian pig (obese). These differences may be related to the fatness or leanness in these two pig breeds. The results provide new leads for further investigations on the fat storage of pigs or even humans.

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Section: Introductionmentioning

confidence: 99%

Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs

Luo

Wright

et al. 2012

Archaea

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“…In most cases the QTL were located in the distal region around the peroxisome proliferative activated receptor gamma coactivator 1 (PGCMUT or PPARGC1). PPARGC1 is a candidate gene that regulates the determination of myofibre types and has an important influence on myofibre growth (Jiang et al 2011). In the study of Jiang et al (2011), strong differences in gene expression between Landrace pigs and Chinese Meishans were reported.…”

Section: Resultsmentioning

confidence: 99%

“…PPARGC1 is a candidate gene that regulates the determination of myofibre types and has an important influence on myofibre growth (Jiang et al 2011). In the study of Jiang et al (2011), strong differences in gene expression between Landrace pigs and Chinese Meishans were reported. The detected QTL on SSC10 were all located in one region near the growth arrest-specific protein 1 marker (GAS1).…”

Section: Resultsmentioning

confidence: 99%

Mapping QTL for growth and muscling traits in three connected porcine F<sub>2</sub> crosses

et al. 2012

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QTL experiments in pigs are often analysed separately, although similar or same founder breeds are frequently used to establish the experimental design. The aim of the present study was to jointly analyse three porcine F 2 -crosses for six growth and four muscling traits. The crosses were a Meishan × Pietrain cross, a Wild Boar × Pietrain cross, and a Wild Boar × Meishan cross. In some cases, same founder animals were used to establish the crosses. 966 F 2 -individuals were genotyped for 242 genetic markers (mostly microsatellites) and phenotyped for birth weight, 21 and 35 day weight, slaughter weight, carcass length, food conversion ratio, ham meat weight, shoulder meat weight, loin and neck meat weight, and meat area. A multi-allele multi-QTL model was applied that estimated an additive QTL effect for each founder breed and parental origin (either paternally or maternally derived), and a dominant QTL effect for each cross. This model was previously introduced in plant breeding. Numerous QTL were mapped on the autosomes. Most QTL were localised on SSC1, 2, 3, 4, 6 and 8, and no QTL were on SSC9, 11, 13, 15, 17 and 18. The confidence intervals were short in many cases. QTL with an exceptionally high test statistic were found for carcass length on SSC1, 4, 7 and 17. The coefficient of variation was remarkably small for this trait, which suggests that carcass length is affected by only a few genes with large effects. Positional and functional candidates underlying promising QTL are suggested for further study.

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Single Nucleotide Polymorphism Scanning and Expression of the Pig PPARGC1A Gene in Different Breeds

Wang

Zhang

et al. 2014

Lipids

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Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a candidate gene for lean meat production because it plays a key role in lipid metabolism. In this study, SNPs within the porcine PPARGC1A gene were investigated using PCR-sequencing and PCR-RFLP. Quantitative real-time PCR and Western blot were then used to analyze mRNA and protein expression in longissimus dorsi muscle (LM), liver, and backfat tissues of Dianna small-ear pigs (DSP, n = 6), Tibetan pigs (TP, n = 6), and large white pigs (LW, n = 6). Five novel SNPs (g.-1269A>G in the 5'-upstream regulatory region; g.190C>T, g.218C>A and g.234C>A in exon 8; and g.20C>T in intron 10) and three previously identified SNPs (g.417A>T in exon 8; g.56C>A in exon 9; and g.34G>A in intron 9) were found. Of these, only two, g.-1269A>G and g.234C>A, had three different genotypes in the three breeds (DSP, n = 63; TP, n = 51; and LW, n = 52). Expression was highest in LM, modest in the liver, and minimal in backfat. In LM tissue, LW had higher mRNA and protein levels than DSP and TP (P < 0.05), and there was a negative correlation between gene expression and intramuscular fat (IMF) content. LW had numerically higher expression in liver and backfat tissues than DSP and TP, and the differences in protein levels were significant (P < 0.05 in liver, P < 0.01 in backfat). In conclusion, PPARGC1A may play a key role in down-regulating lipid deposition, and the SNPs with differential genotype distribution among the three pig breeds may be related to gene expression and fat deposition.

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Expression Changes of PPARGC1A During the Development of Lean and Obese Pigs

Cited by 3 publications

References 6 publications

Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs

Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs

Mapping QTL for growth and muscling traits in three connected porcine F<sub>2</sub> crosses

Single Nucleotide Polymorphism Scanning and Expression of the Pig PPARGC1A Gene in Different Breeds

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Expression Changes of PPARGC1A During the Development of Lean and Obese Pigs

Cited by 3 publications

References 6 publications

Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs

Lean Breed Landrace Pigs Harbor Fecal Methanogens at Higher Diversity and Density than Obese Breed Erhualian Pigs

Mapping QTL for growth and muscling traits in three connected porcine F&lt;sub&gt;2&lt;/sub&gt; crosses

Single Nucleotide Polymorphism Scanning and Expression of the Pig PPARGC1A Gene in Different Breeds

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Mapping QTL for growth and muscling traits in three connected porcine F<sub>2</sub> crosses