Expression characteristics of MMP-2 and MMP-9 in guinea pig ovaries during the estrous cycle

Li, J R; Shen, Ting

doi:10.4238/2015.december.21.1

Cited by 5 publications

(7 citation statements)

References 34 publications

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“…In addition, the higher frequency of membrane opening period (between 1 and 3 days) corresponded to the study by Araníbar and Echevarría (2014). Macroscopic observation of the vulva showed morphological differences, such as swelling, redness and secretion, like those described by Li and Shen (2015).…”

Section: Discussionsupporting

confidence: 70%

“…The duration of the interval in openings showed a variation between 7 and 21 days, occurring with a higher frequency to 17 and 18 days, higher than those presented in literature, which describe cycles between 15 and 17 days (Lilley et al, 1997;Stockard and Papanicolaou, 1919;Hubrecht, 2010), or between 14 and 16 days (Kühnel and Mendoza, 1992;Wang et al, 2010;Li and Shen, 2015).…”

Section: Discussionmentioning

confidence: 57%

“…Vaginal cytology proved to be reliable for the characterization of the estrous cycle. There was distribution of cell types of the vaginal epithelium in each phase, as in the study by Li and Shen (2015). During proestrus, intermediary cells were observed in a higher number, with moderate presence of mucus.…”

Section: Discussionmentioning

confidence: 66%

“…These species are polyestrous and non-seasonal, with spontaneous ovulation and corpus luteum with active secreting activity, with estrous cycle varying between 14 and 16 days (Kühnel and Mendoza, 1992;Wang et al, 2010;Li and Shen, 2015), or 15 and 17 days (Lilley et al, 1997;Hubrecht, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…In mature females, the membrane remains open for one to three days during estrus, but in juvenile females this phase can last longer (Araníbar and Echevarría, 2014). According to Li and Shen (2015), vulvar morphological characteristics in Guinea pigs suffer small alterations in between the different phases of the estrous cycle: swelling, redness, and secretion during proestrus and estrus, and partially closed, light-colored vaginal membrane during the metaestrus.…”

Section: Introductionmentioning

confidence: 99%

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Early puberty in short-haired Guinea pigs kept in laboratory animal facilities

et al. 2022

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Lab animals, such as Guinea pigs (Cavia porcellus), are crucial for scientific development, as they play an important role in the development and quality control chain of vaccines and drugs distributed by the Brazilian public health system. Investigating their biological and physiological parameters is fundamental to raise and keep these animals, so the handling of the facilities that hold them can be updated whenever new information comes up, with the well-being of the animals and alignment with the 3 Rs in mind. In the search for understanding reproductive aspects of Guinea pigs, the present study had the main goal of studying puberty by means of estrous cycle analysis in short-haired Guinea pigs. Guinea pigs have a vaginal occlusive membrane that covers the vaginal orifice. Its rupture takes place gradually and naturally, moments before labor and during estrus. The present study followed 42 females as for the presentation of the vaginal occlusive membrane. Once the membranes ruptured spontaneously, a swab was collected to study vaginal cytology. Membrane rupture was observed in 39 females; six females showed membrane rupture with less than 21 days of age (17 to 21 days). Twenty-three females were characterized as being in estrus due to cytology showing a prevalence of anucleated superficial cells. One of these females was younger than 21 days old. The opening of the vaginal occlusive membrane took place most frequently in intervals between 17 and 18 days, and the membrane remained open between one and three consecutive days. It was possible to follow three cycles of membrane opening on six females. The present study showed the need to adapt handling guidelines for C. porcellus kept in research animal facilities. The early age of puberty imposes the need of separate the female daughters from their fathers at 16 days old.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Early puberty in short-haired Guinea pigs kept in laboratory animal facilities

et al. 2022

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Nuclear factor kappa B signaling within the rat nucleus accumbens core sex-dependently regulates cue-induced cocaine seeking and matrix metalloproteinase-9 expression

Namba¹,

Phillips²,

Neisewander³

2022

Brain, Behavior, and Immunity

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Skin microbiome profiling reveals the crucial role of microbial metabolites in anti‐photoaging

Li,

Chen,

Xie

et al. 2024

Photoderm Photoimm Photomed

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BackgroundSkin microbiota is essential for health maintenance. Photoaging is the primary environmental factor that affects skin homeostasis, but whether it influences the skin microbiota remains unclear.ObjectiveThe objective of this study is to investigate the relationship between photoaging and skin microbiome.MethodsA cohort of senior bus drivers was considered as a long‐term unilateral ultraviolet (UV) irradiated population. 16S rRNA amplicon sequencing was conducted to assess skin microbial composition variations on different sides of their faces. The microbiome characteristics of the photoaged population were further examined by photoaging guinea pig models, and the correlations between microbial metabolites and aging‐related cytokines were analyzed by high‐throughput sequencing and reverse transcription polymerase chain reaction.ResultsPhotoaging decreased the relative abundance of microorganisms including Georgenia and Thermobifida in human skin and downregulated the generation of skin microbe‐derived antioxidative metabolites such as ectoin. In animal models, Lactobacillus and Streptobacillus abundance in both the epidermis and dermis dropped after UV irradiation, resulting in low levels of skin antioxidative molecules and leading to elevated expressions of the collagen degradation factors matrix metalloproteinase (MMP)‐1 and MMP‐2 and inflammatory factors such as interleukin (IL)‐1β and IL‐6.ConclusionsSkin microbial characteristics have an impact in photoaging and the loss of microbe‐derived antioxidative metabolites impairs skin cells and accelerates the aging process. Therefore, microbiome‐based therapeutics may have potential in delaying skin aging.

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Expression characteristics of MMP-2 and MMP-9 in guinea pig ovaries during the estrous cycle

Cited by 5 publications

References 34 publications

Early puberty in short-haired Guinea pigs kept in laboratory animal facilities

Early puberty in short-haired Guinea pigs kept in laboratory animal facilities

Nuclear factor kappa B signaling within the rat nucleus accumbens core sex-dependently regulates cue-induced cocaine seeking and matrix metalloproteinase-9 expression

Skin microbiome profiling reveals the crucial role of microbial metabolites in anti‐photoaging

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