Expression dynamics of vascular endothelial markers: endoglin and syndecan-1 in predicting dengue disease outcome

Mariappan, Vignesh; Adikari, Shalinda; Lokesh, S.; Easow, Joshy M; Pillai, Agieshkumar Balakrishna

doi:10.1016/j.trsl.2021.02.001

Cited by 21 publications

(23 citation statements)

References 50 publications

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“…Thus, TSP2 may be expressed to counterbalance the early inflammatory response induced by LASV in HUVECs. Levels of soluble endoglin (sENG) were also slightly elevated 48 h after infection for both viruses, perhaps because of the activation induced by viral infection, as previously observed with dengue virus [ 67 ]. sENG has been shown to induce a pro-inflammatory phenotype in ECs through the activation of the NFκB/IL-6 pathways [ 68 ].…”

Section: Discussionsupporting

confidence: 54%

Infection of Human Endothelial Cells with Lassa Virus Induces Early but Transient Activation and Low Type I IFN Response Compared to the Closely-Related Nonpathogenic Mopeia Virus

Merabet

Pietrosemoli

Perthame

et al. 2022

Viruses

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Lassa virus (LASV), an Old World arenavirus, is responsible for hemorrhagic fevers in western Africa. The privileged tropism of LASV for endothelial cells combined with a dysregulated inflammatory response are the main cause of the increase in vascular permeability observed during the disease. Mopeia virus (MOPV) is another arenavirus closely related to LASV but nonpathogenic for non-human primates (NHPs) and has never been described in humans. MOPV is more immunogenic than LASV in NHPs and in vitro in human immune cell models, with more intense type I IFN and adaptive cellular responses. Here, we compared the transcriptomic and proteomic responses of human umbilical vein endothelial cells (HUVECs) to infection with the two viruses to further decipher the mechanisms involved in their differences in immunogenicity and pathogenicity. Both viruses replicated durably and efficiently in HUVECs, but the responses they induced were strikingly different. Modest activation was observed at an early stage of LASV infection and then rapidly shut down. By contrast, MOPV induced a late but more intense response, characterized by the expression of genes and proteins mainly associated with the type I IFN response and antigen processing/presentation. Such a response is consistent with the higher immunogenicity of MOPV relative to LASV, whereas the lack of an innate response induced in HUVECs by LASV is consistent with its uncontrolled systemic dissemination through the vascular endothelium.

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Section: Discussionsupporting

confidence: 54%

Infection of Human Endothelial Cells with Lassa Virus Induces Early but Transient Activation and Low Type I IFN Response Compared to the Closely-Related Nonpathogenic Mopeia Virus

Merabet

Pietrosemoli

Perthame

et al. 2022

Viruses

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“…For example, endoglin is an endothelial marker that is highly expressed during the inflammation process. The soluble form of endoglin (sEng) is released into circulation by activated cells and is reported to be potential markers for vascular leakage in dengue disease severity [ 101 , 102 ]. Similar to Eng, another important marker of vascular endothelial activations called SDC-1 is reported to induce neutrophil chemotaxis, inhibit alveolar epithelial wound healing, and promote pulmonary fibrosis [ 103 ].…”

Section: Endothelial Markersmentioning

confidence: 99%

Potential biomarkers for the early prediction of SARS-COV-2 disease outcome

Mariappan

Manoharan

Ranganadin

et al. 2021

Microbial Pathogenesis

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The current pandemic due to the fast spreading of SARS-CoV-2 infection has caused severe impairment in health, social, economic, scientific, and medical sectors across the globe. Owing to the not so well understood mechanism of disease pathogenesis in terms of variations in immune responses, there remains obscure why some of the patients who are infected by the novel SARS-CoV-2 develop an unpredictable clinical course that rapidly causes severe and deadly complications/manifestations. Currently, several assays are available for the confirmation of SARS-CoV-2 infection at the point of care. However, none of these assays can predict the severity of the COVID-19 disease. Thus, the identification of a prognostic biomarker that forecasts the condition of SARS-CoV-2 patients to develop a severe form of the disease could enable the clinicians for more efficient patient triage and treatment. In this regard, the present review describes the role of selected biomolecules that are crucially involved in the immune-pathogenesis of SARS-CoV-2 infection such as hyper-immune responsiveness, bradykinin storm and vascular leakage assuming these may serve as an effective prognostic biomarker in COVID-19 to understand the outcome of the disease. Based on the review, we also propose the development of a cost-effective SERS-based prognostic biosensor for the detection and quantification of biomolecules for use as a point-of-care system during a disease outbreak.

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“…In dengue, impaired platelet aggregation to ADP from dengue antigen immune complexes formation on platelet surfaces have been reported [ 23 , 24 ]. A recent study analyzing the expression of endoglin (EDG) and Syndecan-1 (SDC1) from activated endothelial cells during plasma leakage in critical phase of dengue showed significant correlation in predicting dengue severity [ 25 ]. Hence, significant association of raised hematocrit with ROTEM abnormalities as found in this study has further potential for translational research in the future.…”

Section: Discussionmentioning

confidence: 99%

Correlation of Dengue Warning Signs during Febrile Phase with Rotational Thromboelastometry, Cortisol and Feritin

Abas

Karim

Periyasamy

et al. 2022

IJERPH

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Dengue mortality remains high despite monitoring against warning signs (WS). The associations of WS at febrile phase (FP) and hemorrhage at defervescence with the levels and kinetics of ROTEM, platelet count, cortisol, and ferritin were analyzed. Patients with confirmed dengue serology and WS in two centers were screened (n = 275) and 62 eligible patients were recruited prospectively over 9 months. “Vomiting” was the commonest WS (62.9%), with shortened clotting time (CT) INTEM (p = 0.01). “Hematocrit increase” showed significant prolonged CT INTEM, EXTEM, and FIBTEM (p < 0.05). “Platelet decrease” showed reduced platelet function and reduced clot amplitude at 10 min (A10) and maximum clot firmness (MCF) in INTEM and EXTEM (p < 0.001). The kinetics were reduced in platelet count, CT EXTEM, and cortisol (p < 0.05) but increased in CT INTEM (p = 0.03). At FP, “vomiting”, “hematocrit increase”, and “platelet decrease” demonstrated impaired CT, clot strengths A10/MCF and platelet functions. Majority (60/62, 96.7%) had non-severe outcomes, consistent with increase in cortisol kinetics. In conclusion, “vomiting”, “hematocrit increase” and “platelet decrease” at FP correlated with ROTEM. No conclusion could be made further regarding ferritin and cortisol. Larger study is required to study “hematocrit increase” with ROTEM as a potential marker for hemorrhage.

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Expression dynamics of vascular endothelial markers: endoglin and syndecan-1 in predicting dengue disease outcome

Cited by 21 publications

References 50 publications

Infection of Human Endothelial Cells with Lassa Virus Induces Early but Transient Activation and Low Type I IFN Response Compared to the Closely-Related Nonpathogenic Mopeia Virus

Infection of Human Endothelial Cells with Lassa Virus Induces Early but Transient Activation and Low Type I IFN Response Compared to the Closely-Related Nonpathogenic Mopeia Virus

Potential biomarkers for the early prediction of SARS-COV-2 disease outcome

Correlation of Dengue Warning Signs during Febrile Phase with Rotational Thromboelastometry, Cortisol and Feritin

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