Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate

Comai, Luca; Facciotti, Daniel; Hiatt, William R.; Thompson, Gregory A.; Rose, Ronald E.; Stalker, David M.

doi:10.1038/317741a0

Cited by 351 publications

(91 citation statements)

References 36 publications

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“…(g fresh weight) -1 of distilled water, and the crude extracts were analyzed to detect the enzyme that confers resistance to glufosinate (PAT, phosphinothricin-N-acetyltransferase; Thompson et al, 1987) or the enzyme that confers resistance to glyphosate (EPSPS, 5-enolpyruvylshikimate-3-phosphate synthase; Comai et al, 1985), respectively, using the TraitChek LL Test Kit (Strategic Diagnostic Inc., Newark, DE, USA) and the Reveal kit (Neogen, Lansing, MI, USA). For DNA analyses, DNA was extracted from another leaf of the herbicide-resistant plant using the SDS-phenol method (Nakajima et al, 1988) and analyzed by means of PCR, using the primers 5'-TCGTCAACCACTACATCGAGACA-3' and 5'-GAAACCCACGTCATGCCAGTT-3' for the bar gene that encodes PAT, and using 5'-AAGAACTC-CGCGTCAAGGAAAGCGA-3' and 5'-AGCCTTCG-TATCGGAGAGTTCGAT-3' for the cp4 epsps gene that encodes EPSPS.…”

Section: Biochemical Analysesmentioning

confidence: 99%

Monitoring the escape of transgenic oilseed rape around Japanese ports and roadsides

Saji

Nakajima

Aono

et al. 2005

Environ. Biosafety Res.

100

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An investigation was carried out to monitor the escape and spread of oilseed rape (Brassica napus) transgenic plants and the introgression of transgenes to its closely related feral species in Japan. We screened a total of about 7500 feral B. napus, 300 B. rapa, and 5800 B. juncea seedlings from maternal plants in 143 locations at several ports, roadsides, and riverbanks. The presence of glufosinate-resistance or glyphosate-resistance transgenes in these seedlings was confirmed by means of herbicide treatments and also immunochemical and DNA analyses. B. napus plants with herbicide-resistant transgenic seeds were found at five of six major ports and along two of four sampled roadsides in the Kanto District. Transgenic oilseed rape plants have not been commercially cultivated in Japan, suggesting that the transgenes would probably have come from imported transgenic seeds that were spilled during transportation to oilseed processing facilities. No transgenes were detected in seeds collected from B. napus plants growing along riverbanks in the Kanto District or in seeds from closely related species (B. rapa and B. juncea). To our knowledge, this is the first published example of feral, transgenic populations occurring in a nation where the transgenic crop has not been cultivated commercially.

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Section: Biochemical Analysesmentioning

confidence: 99%

Monitoring the escape of transgenic oilseed rape around Japanese ports and roadsides

Saji

Nakajima

Aono

et al. 2005

Environ. Biosafety Res.

100

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“…For our biochemical analyses by means of immunochemical chromatography, we homogenized 10 mg of lyophilized leaves with a mortar and pestle in 200 µL of distilled water and immunochemically analyzed the crude extracts to detect proteins that confer resistance to glyphosate (5-enolpyruvylshikimate-3-phosphate synthase, EPSPS; Comai et al, 1985) or glufosinate (phosphinothricin-N-acetyltransferase, PAT; Thompson et al, 1987) using the Reveal kit (Neogen, Lansing, MI, USA) and the TraitChek LL Test Kit (Strategic Diagnostic Inc., Newark, DE, USA), respectively.…”

Section: Detection Of Herbicide-resistance Transgenesmentioning

confidence: 99%

Monitoring the occurrence of genetically modified oilseed rape growing along a Japanese roadside: 3-year observations

Nishizawa

Nakajima

Aono

et al. 2009

Environ. Biosafety Res.

101

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“…Selective breeding still plays a major role in the production of new varieties but the emergence of modern plant biotechnology has led to a more targeted approach to increasing crop yields. Traits of agricultural importance successfully introduced to plants using recombinant DNA technology include herbicide resistance (Comai et al 1985), drought resistance (Kumar et al 2014), pest resistance (Bates et al 2005), pathogen resistance (Brunner et al 2011;Horvath et al 2012;Jones et al 2014), abiotic stress resistance (Jaglo-Ottosen et al 1998), enhanced photosynthetic capacity (Ku et al 2001), im-proved nitrogen use efficiency (Yanagisawa et al 2004), and added nutritional value (Ye et al 2000). However, although plants hold a unique promise for bioproduction at the gigatonne scale, efforts in genetic engineering of plants are lagging compared with microbial systems (Antunes et al 2009(Antunes et al , 2011Liu et al 2011Liu et al , 2013Koschmann et al 2012;Wend et al 2013;Zurcher et al 2013;Fethe et al 2014;Mül-ler et al 2014).…”

mentioning

confidence: 99%

Synthetic Botany

Boehm

Pollak

Purswani³

et al. 2017

Cold Spring Harb Perspect Biol

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Plants are attractive platforms for synthetic biology and metabolic engineering. Plants' modular and plastic body plans, capacity for photosynthesis, extensive secondary metabolism, and agronomic systems for large-scale production make them ideal targets for genetic reprogramming. However, efforts in this area have been constrained by slow growth, long life cycles, the requirement for specialized facilities, a paucity of efficient tools for genetic manipulation, and the complexity of multicellularity. There is a need for better experimental and theoretical frameworks to understand the way genetic networks, cellular populations, and tissue-wide physical processes interact at different scales. We highlight new approaches to the DNA-based manipulation of plants and the use of advanced quantitative imaging techniques in simple plant models such as Marchantia polymorpha. These offer the prospects of improved understanding of plant dynamics and new approaches to rational engineering of plant traits.

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Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate

Cited by 351 publications

References 36 publications

Monitoring the escape of transgenic oilseed rape around Japanese ports and roadsides

Monitoring the escape of transgenic oilseed rape around Japanese ports and roadsides

Monitoring the occurrence of genetically modified oilseed rape growing along a Japanese roadside: 3-year observations

Synthetic Botany

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