Expression level of miRNAS in patients with gestational diabetes

Abstract

BackgroundGestational diabetes mellitus (GDM), which has been identified for the first time during pregnancy and is defined as glucose intolerance of varying degrees, is one of the prediabetes states that are at risk for developing type 2 diabetes. MicroRNAs (miRNAs) are associated with pregnancy complications and are even necessary for the regulation of pancreatic development and glucose-induced insulin secretion. This study aims to investigate miRNAs expressed in maternal plasma to detect the availability as a biomarker in the diagnosis of GDM.MethodsIn this study, 30 pregnant women with GDM diagnosed according to GDM American Diabetes Association criteria and 30 healthy pregnant women were included and miRNA expression levels were investigated. Circulating 39 miRNAs were analyzed by qRT-PCR using 96.96 Dynamic Array IFCs.ResultsThe findings showed that the expression level of miR-7-5p significantly upregulated when compared with healthy pregnant women with gestational diabetes (fold change:2.3, p<0.05).ConclusionsThere is controversy about what is the most appropriate test for GDM screening. Therefore, supported by further studies, miR-7-5p can be used for the diagnosis of GDM.

A Big Role for microRNAs in Gestational Diabetes Mellitus

“…Of the three studies, one study reported higher levels of miR-9 in the serum of Mexican women with GDM compared to controls (49). In contrast, two studies profiling miRNAs in placental tissue of Chinese women (46) and in plasma samples of Turkish women (32) reported lower expression of miR-9 in women with GDM compared to controls. Of the seven studies reporting on miR-16, three studies demonstrated higher expression in serum and plasma samples of Chinese and European women with GDM compared to controls (34,62,83).…”

“…Two studies reported on the expression of miR-142 and miR-143 during GDM. One study reported higher expression of miR-142 and miR-143 in plasma of Turkish women with GDM compared to controls (32). Stirm et al (60) reported higher expression of miR-142 and miR-143 in the whole blood of German women with GDM in the screening group, however, these findings were not validated in a larger sample.…”

“…Four studies investigated miR-17 during GDM. Of these, three studies reported that miR-17 expression was higher in plasma samples of Chinese and Turkish women with GDM compared to controls (32,34,83). In contrast, Pheiffer et al (55) showed no significant difference in miR-17 expression in the serum of South African women with GDM compared to controls (55).…”

“…Two studies reported contradicting results for miR-125b. Lamadrid-Romero et al (45) reported higher expression of miR-125b in serum samples of Mexican women with GDM compared to controls (45), while Balci et al (32) reported lower expression in plasma samples of Turkish women with GDM compared to controls. Of the five studies, only one study reported a higher expression of miR-132 in the serum samples of Canadian women (38).…”

Recent Updates on microRNA as Potential Biomarkers for Diagnosis of Gestational Diabetes mellitus

“…Moreover, Yoffe et al found upregulated levels of miR-223 & miR-23a in GDM patients during the first trimester of pregnancy, which might guide to develop of a non-invasive prenatal testing (NIPT) method during the first or early second trimester of pregnancy to manage GDM effectively and to prevent related risks on morphogenesis and organogenesis (1). Finally, Balci et al also reported increased miR-7-5p levels during the late second trimester of pregnancy in women with GDM (18). The key findings of recent literature on miR as GDM biomarker have been summarized in table 1.…”