Expression level of NUAK1 in human nasopharyngeal carcinoma and its prognostic significance

Liu, Jiaoyang; Tang, Guoyan; Huang, He; Li, Huan; Zhang, Peng; Xu, Lihua

doi:10.1007/s00405-018-5095-0

Cited by 20 publications

(18 citation statements)

References 21 publications

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“…In certain geographic and ethnic populations,nasopharyngeal carcinoma (NPC) registers high incidence rates, that still lacks relevant biomarkers for its development and prognosis; therefore, studying more biomarkers are going to predict of the prognosis of (NPC) andestablishment of targeted therapeutic approaches [18] .…”

Section: Discussionmentioning

confidence: 99%

Tight Junction Protein (Claudin-4) and Inflammatory Regulator (COX-2) Expression in Nasopharyngeal Carcinoma: An Immunohistochemical Study

Abdrabuh

Roshdy

2019

The Medical Journal of Cairo University

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Background: Claudin 4 is one of the tight junction proteins which considered one of the protective barriers that are mostly involved in the regulation of intercellular communication, paracellular transport and apical cell-to-cell adhesion. Overexpression of COX-2 and subsequent increase in prostaglandins (PGs) concentration have emerged in recent years as important players in cancer progression. Aim of Study:To explore immunohisto chemically the expression of both claudin-4 and COX2 in nasopharyngeal carcinoma and correlate their expression with each other and with different clinicopathological parameters. Material and Methods:This retrospective study was done upon formalin fixed, paraffin blocks of 32 nasopharngeal carcinoma cases and 6 non-neoplastic nasopharyngeal epithelium. The materials were obtained from archieves of Pathology Departement and Early Cancer Detection Unit (ECDU), Faculty of Medicine, Benha University during the years 2012-2017. The immunohistochemical stain with Claudin 4 and COX2 was done and evaluated for each case.Results: High expression of claudin 4 was observed in 44% of studied nasopharyngeal cases with statistically significant correlation with tumor stage and distant metastasis (p<0.05). Positive expression of COX2 was detected in 75% of neoplastic group and statistically correlated with depth of tumor invasion, nodal invasion and tumor stage (p<0.05). A statistically significant correlation was detected between overexpression of COX2 and low expression of claudin4 in the studied neoplastic cases (p<0.05). Conclusion:Claudin -4 has different tumorigenic effects besides their known adhesion attributes. Because of that, we believe that claudin 4 with other clinicopahological parameters may be a promising prognostic molecular indicator in nasopharyngeal cancer cases and can be taken as a novel biomarker for the predication of distant metastasis and survival. This work also highlights COX-2 expression's potential as an indicator of cancer progression that can contribute to assessment of prognosis and treatment decisions. Therefore, COX -2 inhibitors may have considerable potential as therapeutic agents.

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Section: Discussionmentioning

confidence: 99%

Tight Junction Protein (Claudin-4) and Inflammatory Regulator (COX-2) Expression in Nasopharyngeal Carcinoma: An Immunohistochemical Study

Abdrabuh

Roshdy

2019

The Medical Journal of Cairo University

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“…37 Following an analogous logic, the hsa_circ_0028007 studied here was originated from NUAK1 gene (Table S1), whose excessive expression was correlated with poor disease-free survival of NPC patients. 38 Besides NPC, aberrant over-expression of NUAK1 was also associated with unfavorable survival of patients who were plagued by non-small-cell lung cancer, primary hepatocellular carcinoma, glioma, and melanoma. [39][40][41][42] These clini- In addition, paclitaxel and cisplatin were commonly applied for treating patients with advanced NPC, and their anticancer mechanism differed from each other.…”

Section: F I G U R Ementioning

confidence: 99%

Implication of hsa_circ_0028007 in reinforcing migration, invasion, and chemo‐tolerance of nasopharyngeal carcinoma cells

Dong

Zhang

Hao

et al. 2020

Clinical Laboratory Analysis

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Background Given the reliability of circRNAs in symbolizing cancer progression, this investigation was designed to expound the involvement of hsa_circ_0028007 in regulating chemosensitivity of nasopharyngeal carcinoma (NPC) cells. Methods Altogether, 241 pairs of NPC tissues and para‐cancerous normal tissues were collected to identify NPC‐symbolic circRNAs, which have been screened by circRNA microarray in advance. Expressions of the circRNAs were determined by means of real‐time polymerase chain reaction (PCR). Besides, human NPC cell lines (ie, CNE2 and HONE1) were transfected by si‐hsa_circ_0028007 and si‐NC. Scratch assay, transwell assay, and MTT assay were performed to assess migration, invasion, and paclitaxel/cisplatin‐resistance of NPC cell lines. Results Hsa_circ_0028007 expression was abnormally heightened within NPC tissues in comparison with matched non‐tumor tissues (P < .05). Over‐expressed hsa_circ_0028007 was strongly associated with advanced (III‐IV) tumor stage, aggressive infiltration, and metastatic lymph nodes of NPC patients (P < .05). Regarding in vitro experiments, hsa_circ_0028007 expression was elevated in CNE2 and HONE1 cell lines as compared with HENE cell line (P < .05). Silencing of hsa_circ_0028007 not merely sensitized CNE2 and HONE1 cells against paclitaxel and cisplatin (P < .05), but also significantly repressed migration and invasion of the cell lines (P < .05). Conclusion Hsa_circ_0028007 was involved in facilitating progression and chemo‐resistance of NPC, which might offer an alternative for NPC treatment.

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“…ARK5, a member of the AMPK family, has been suggested to be closely related to cell survival and cell function of tumors (Chang et al, 2013). Moreover, the relationships between ARK5 and cancer cells have been verified in many cancer types-for instance, cholangiocarcinoma (Yu et al, 2017), hepatoma (Xu et al, 2016) and nasopharyngeal carcinoma (Liu et al, 2018). The upstream mediator of ARK5 has been identified as AKT (Suzuki et al, 2004), LBK1 (Chang et al, 2013), CaMKKb (Hawley et al, 2003) and TAK1 (Momcilovic, Hong & Carlson, 2006).…”

Section: Introductionmentioning

confidence: 99%

Silencing of the ARK5 gene reverses the drug resistance of multidrug-resistant SGC7901/DDP gastric cancer cells

Wan

Liu

Chen

et al. 2020

PeerJ

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For several years, the multidrug resistance (MDR) of gastric cancer cells has been a thorny issue worldwide regarding the chemotherapy process and needs to be solved. Here, we report that the ARK5 gene could promote the multidrug resistance of gastric cancer cells in vitro and in vivo. In this study, LV-ARK5-RNAi lentivirus was used to transfect the parental cell line SGC7901 and MDR cell line SGC7901/DDP to construct a stable model of ARK5 interference. Subsequently, the cells were treated with four chemotherapeutic drugs, cisplatin (DDP), adriamycin (ADR), 5-fluorouracil (5-FU) and docetaxel (DR) and were subjected to the CCK8, colony formation, adriamycin accumulation and retention, cell apoptosis and other assays. The study found that, in vitro, the expression of ARK5 in MDR gastric cancer cells was significantly higher than that in parental cells. Additionally, when treated with different chemotherapeutic drugs, compared with parental cells, MDR cells also had a higher cell survival rate, higher colony formation number, higher drug pump rate, and lower cell apoptosis rate. Additionally, in xenograft mouse models, MDR cells with high ARK5 expression showed higher resistance to chemotherapeutic drugs than parental cells. Overall, this study revealed that silencing the ARK5 gene can effectively reverse the drug resistance of MDR gastric cancer cells to chemotherapeutic drugs, providing insights into the mechanism of this process related to its inhibition of the active pump-out ability of MDR cells.

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Expression level of NUAK1 in human nasopharyngeal carcinoma and its prognostic significance

Cited by 20 publications

References 21 publications

Tight Junction Protein (Claudin-4) and Inflammatory Regulator (COX-2) Expression in Nasopharyngeal Carcinoma: An Immunohistochemical Study

Tight Junction Protein (Claudin-4) and Inflammatory Regulator (COX-2) Expression in Nasopharyngeal Carcinoma: An Immunohistochemical Study

Implication of hsa_circ_0028007 in reinforcing migration, invasion, and chemo‐tolerance of nasopharyngeal carcinoma cells

Silencing of the ARK5 gene reverses the drug resistance of multidrug-resistant SGC7901/DDP gastric cancer cells

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