Expression, localisation and phylogeny of a novel family of plant‐specific membrane proteins

Kasaras, Alexis; Kunze, Reinhard

doi:10.1111/j.1438-8677.2010.00381.x

Cited by 26 publications

(39 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DMPs represent a unique family of plant-specific plasma membrane proteins of unknown function that have been recently identified in a screen for senescence-associated genes in Arabidopsis (Kasaras and Kunze, 2010). Of the 10 Arabidopsis DMP paralogs, DMP4 is coregulated with the core of dPCD marker genes and up-regulated in several dPCD conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Of the 10 Arabidopsis DMP paralogs, DMP4 is coregulated with the core of dPCD marker genes and up-regulated in several dPCD conditions. Additionally, expression of DMP4 has been described in abscission zones of floral organs (Kasaras and Kunze, 2010), although involvement of PCD in this abscission process has not been investigated. The molecular function of DMP proteins still needs to be determined, but misexpression of Arabidopsis DMP1 led to an aberrant endoplasmic reticulum and in some cases to the death of transfected cells (Kasaras et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A conserved core of PCD indicator genes discriminates developmentally and environmentally induced programmed cell death in plants

et al. 2015

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A conserved core of PCD indicator genes discriminates developmentally and environmentally induced programmed cell death in plants

et al. 2015

View full text Add to dashboard Cite

“…The fragment of SWEET12 comprising a 1887-bp SWEET12 promoter sequence and 1858 bp of the coding region up to but not including the stop codon was amplified with the forward primer AtSWT12KpnIF containing a KpnI restriction site and the reverse primer AtSWT12PstIR containing a PstI restriction site and subcloned into the eGFP fusion vector pGTKan3 (Kasaras and Kunze, 2010) via KpnI and PstI restriction sites. A similar strategy was applied for SWEET15.…”

Section: Egfp Fusion Constructs Under Native Promotersmentioning

confidence: 99%

A Cascade of Sequentially Expressed Sucrose Transporters in the Seed Coat and Endosperm Provides Nutrition for the Arabidopsis Embryo

et al. 2015

View full text Add to dashboard Cite

Developing plant embryos depend on nutrition from maternal tissues via the seed coat and endosperm, but the mechanisms that supply nutrients to plant embryos have remained elusive. Sucrose, the major transport form of carbohydrate in plants, is delivered via the phloem to the maternal seed coat and then secreted from the seed coat to feed the embryo. Here, we show that seed filling in Arabidopsis thaliana requires the three sucrose transporters SWEET11, 12, and 15. SWEET11, 12, and 15 exhibit specific spatiotemporal expression patterns in developing seeds, but only a sweet11;12;15 triple mutant showed severe seed defects, which include retarded embryo development, reduced seed weight, and reduced starch and lipid content, causing a "wrinkled" seed phenotype. In sweet11;12;15 triple mutants, starch accumulated in the seed coat but not the embryo, implicating SWEETmediated sucrose efflux in the transfer of sugars from seed coat to embryo. This cascade of sequentially expressed SWEETs provides the feeding pathway for the plant embryo, an important feature for yield potential.

show abstract

“…The 3-kb Ds element from pD16 was excised with PstI and PauI, blunted with T4-DNA-Polymerase (Fermentas), and inserted into the SmaI restriction site of pPGTKan3 (Kasaras and Kunze 2010). pD16 was derived from pKU4 (Baker et al 1987) by insertion of the Ds element into the BamHI restriction site of pUC19.…”

Section: Construction Of Binary Vectorsmentioning

confidence: 99%

A Hyperactive Transposase of the Maize Transposable ElementActivator(Ac)

Lazarow

Weimer

et al. 2012

Genetics

View full text Add to dashboard Cite

Activator/Dissociation (Ac/Ds) transposable elements from maize are widely used as insertional mutagenesis and gene isolation tools in plants and more recently also in medaka and zebrafish. They are particularly valuable for plant species that are transformation-recalcitrant and have long generation cycles or large genomes with low gene densities. Ac/Ds transposition frequencies vary widely, however, and in some species they are too low for large-scale mutagenesis. We discovered a hyperactive Ac transposase derivative, AcTPase 4x , that catalyzes in the yeast Saccharomyces cerevisiae 100-fold more frequent Ds excisions than the wild-type transposase, whereas the reintegration frequency of excised Ds elements is unchanged (57%). Comparable to the wild-type transposase in plants, AcTPase 4x catalyzes Ds insertion preferentially into coding regions and to genetically linked sites, but the mutant protein apparently has lost the weak bias of the wild-type protein for insertion sites with elevated guanine-cytosine content and nonrandom protein-DNA twist. AcTPase 4x exhibits hyperactivity also in Arabidopsis thaliana where it effects a more than sixfold increase in Ds excision relative to wild-type AcTPase and thus may be useful to facilitate Ac/Ds-based insertion mutagenesis approaches. DNA transposons are widely used in plants and animals as functional genomics tools and gene transfer vehicles. In plants, transposable elements are particularly valuable when large-scale T-DNA insertion mutagenesis is not feasible, e.g., for transformation-recalcitrant species or plants with long generation cycles. The success of transposon insertion mutagenesis strategies depends on high forward mutagenesis rates and a favorable distribution of novel insertions. As forward mutagenesis rates are frequently limited by transposase activity, attempts were made to find hyperactive transposase mutants. For some transposons, such mutants were fortuitously found; in other instances, systematic screening approaches and molecular evolution were successful (Goryshin and Reznikoff 1998;Beall et al. 2002;Baus et al. 2005;Keravala et al. 2006;Mates et al. 2009).The maize Activator/Dissociation (Ac/Ds) transposable elements have been widely used in plants for gene tagging and functional genomics approaches because they are active in numerous plant species, integrate preferentially into or near to coding regions, and frequently transpose to genetically linked sites, enabling local saturation mutagenesis approaches (reviewed in Kunze and Weil 2002). The successful introduction of Ac/Ds elements into yeast revealed that application of these elements is not restricted to plants (Weil and Kunze 2000). Their recent adoption as tools for transgenesis and the generation of gene trap lines in the teleost fishes zebrafish and medaka further emphasized the wide range functionality of Ac/Ds elements (Emelyanov et al. 2006;Boon Ng and Gong 2011;Froschauer et al. 2012).An impediment for a universal application is the variability of Ac/Ds transposition fr...

show abstract

Expression, localisation and phylogeny of a novel family of plant‐specific membrane proteins

Cited by 26 publications

References 40 publications

A conserved core of PCD indicator genes discriminates developmentally and environmentally induced programmed cell death in plants

A conserved core of PCD indicator genes discriminates developmentally and environmentally induced programmed cell death in plants

A Cascade of Sequentially Expressed Sucrose Transporters in the Seed Coat and Endosperm Provides Nutrition for the Arabidopsis Embryo

A Hyperactive Transposase of the Maize Transposable ElementActivator(Ac)

Contact Info

Product

Resources

About