Expression of 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase (CNPase) in the developing olfactory bulb and subventricular zone rostral extension

Gomes, Silvana S.; Carvalho, Sergio L.; Santiago, Marcelo F.; Lopez, Luciane Barreiro; Barradas, Penha C.; Cavalcante, Leny A.

doi:10.1002/jnr.10678

Cited by 9 publications

(6 citation statements)

References 53 publications

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“…2A,D). As expected from previous studies, these CNPase 1 /MR 2 cells in the borders of glomeruli, particularly deep in the tissue, should be identified as oligodendrocytes (Burudi et al, 1999;Gomes et al, 2003).…”

Section: Oecs In Situ Bind a Highly Mannosylated Glycoprotein And Reasupporting

confidence: 81%

The mannose receptor is expressed by olfactory ensheathing cells in the rat olfactory bulb

Carvalho

Nóbrega

Soares

et al. 2013

J of Neuroscience Research

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Complex carbohydrate structures are essential molecules of infectious bacteria, parasites, and host cells and are involved in cell signaling associated with immune responses, glycoprotein homeostasis, and cell migration. The uptake of mannose-tailed glycans is usually carried out by professional phagocytes to trigger MHC class I- and MHC class II-restricted antigen presentation or, alternatively, to end inflammation. We have detected the mannose receptor (MR) in cultured olfactory ensheathing cells (OECs), so we investigated by flow cytometry whether recently dissociated cells of the olfactory bulb (OB) nerve fiber layer (ONL) could bind a mannosylated ligand (fluorescein conjugate of mannosyl bovine serum albumin; Man/BSA-FITC) in a specific manner. In addition, we estimated the relative proportion of ONL OECs, microglia, and astrocytes, tagged by 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNPase), by the B4 isolectin of Griffonia simplicifonia (IB4), and by glial fibrillary acidic protein (GFAP), respectively, that were Man/BSA-FITC(+) . We also determined by histochemistry and/or immunohistochemistry whether Man/BSA-FITC or an anti-MR antibody (anti-C-terminal MR peptide; anti-cMR) labeled OECs and/or parenchymal microglia. In addition, we confirmed by Western blot with the K1K2 (against the entire MR molecule) antibody that a band of about 180 kDA is expressed in the OB. Our findings are compatible with a prospective sentinel role of OECs against pathogens of the upper airways and/or damage-associated glycidic patterns as well as with homeostasis of OB mannosylated glycoproteins.

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Section: Oecs In Situ Bind a Highly Mannosylated Glycoprotein And Reasupporting

confidence: 81%

The mannose receptor is expressed by olfactory ensheathing cells in the rat olfactory bulb

Carvalho

Nóbrega

Soares

et al. 2013

J of Neuroscience Research

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“…Glial progenitors migrate mainly postnatally to the olfactory bulb and the first GFAP and CNP-labelled cells appear around Pn6-7 (Gomes et al, 2003). We detected strong GFAP staining at Pn16 (n = 3) especially in the VZ and glomerular layer in the wild type olfactory bulbs (arrowheads in Fig.…”

Section: Decrease In Oligodendrocytes and Opcs In Ntn1-deficient Obsmentioning

confidence: 77%

“…The anterior part of the SVZ and the RMS are also thought to be a source of oligodendrocytes (Gomes et al, 2003;Gritti et al, 2002;Vitry et al, 2001).…”

Section: Migration Defects In Ntn1−/− Brainmentioning

confidence: 99%

Netrin1 is required for neural and glial precursor migrations into the olfactory bulb

Hakanen

Duprat

Salminen

2011

Developmental Biology

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Netrin1 (NTN1) deficiency in mouse brain causes defects in axon guidance and cell migration during embryonic development. Here we show that NTN1 is required for olfactory bulb (OB) development at late embryogenesis and at early postnatal stages to facilitate the accumulation of proper numbers of granular and glomerular neuron subtypes and oligodendrocytes into the OB. In addition to the analysis of Ntn1-/- mice we made tissue and neurosphere cultures to clarify the role of NTN1 in the anterior forebrain. We propose that a subset of neural progenitors/precursors requires NTN1 to efficiently enter the rostral migratory stream to migrate into the OB. The analysis of postnatal Ntn1-/- OBs revealed a reduction of specific types of interneurons which have been shown to originate from particular subregions of the lateral ventricle walls. Based on Ntn1 expression in ventral parts of the ventricle walls, we observed a decrease in the mainly ventrally derived type II interneurons that express calcium-binding proteins calretinin and calbindin. Instead, no change in the numbers of dorsally derived tyrosine hydroxylase expressing interneurons was detected. In addition to the specific reduction of type II interneurons, our results indicate that NTN1 is required for oligodendroglial migration into the OB. Furthermore, we characterised the Ntn1 expressing subpopulation of neurosphere-forming cells from embryonic and adult brain as multipotent and self-renewing. However, NTN1 is dispensable for the proliferation of neurosphere forming progenitor cells and for their differentiation.

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“…CNPase staining was observed on some, but not all presumptive OECs in explant cultures from the olfactory bulb [24], but not on presumptive OECs in dissociated cultures from the nasal epithelium cultured on astrocyte feeder layers [25]. Immunostaining of developing olfactory bulb focused on CNPase staining of oligodendrocytes and did not report CNPase staining of OECs [26]. It is not clear therefore whether CNPase is expressed by OECs only in specific environments, or whether levels of CNPase may be too low to reliably detect with standard antibody staining protocols.…”

Section: Introductionmentioning

confidence: 99%

CNPase Expression in Olfactory Ensheathing Cells

Radtke

Sasaki

Lankford

et al. 2011

BioMed Research International

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A large body of work supports the proposal that transplantation of olfactory ensheathing cells (OECs) into nerve or spinal cord injuries can promote axonal regeneration and remyelination. Yet, some investigators have questioned whether the transplanted OECs associate with axons and form peripheral myelin, or if they recruit endogenous Schwann cells that form myelin. Olfactory bulbs from transgenic mice expressing the enhanced green fluorescent protein (eGFP) under the control of the 2-3-cyclic nucleotide 3-phosphodiesterase (CNPase) promoter were studied. CNPase is expressed in myelin-forming cells throughout their lineage. We examined CNPase expression in both in situ in the olfactory bulb and in vitro to determine if OECs express CNPase commensurate with their myelination potential. eGFP was observed in the outer nerve layer of the olfactory bulb. Dissociated OECs maintained in culture had both intense eGFP expression and CNPase immunostaining. Transplantation of OECs into transected peripheral nerve longitudinally associated with the regenerated axons. These data indicate that OECs in the outer nerve layer of the olfactory bulb of CNPase transgenic mice express CNPase. Thus, while OECs do not normally form myelin on olfactory nerve axons, their expression of CNPase is commensurate with their potential to form myelin when transplanted into injured peripheral nerve.

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Expression of 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase (CNPase) in the developing olfactory bulb and subventricular zone rostral extension

Cited by 9 publications

References 53 publications

The mannose receptor is expressed by olfactory ensheathing cells in the rat olfactory bulb

The mannose receptor is expressed by olfactory ensheathing cells in the rat olfactory bulb

Netrin1 is required for neural and glial precursor migrations into the olfactory bulb

CNPase Expression in Olfactory Ensheathing Cells

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