Expression of a B-lymphocyte antigen in chronic lymphocytic and other leukemias

Sullivan, Arthur K.; Jerry, L. M.; Rowden, Geoffrey; Shea, M

doi:10.1016/0090-1229(77)90092-7

Cited by 9 publications

(3 citation statements)

References 29 publications

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“…K. Sullivan. Some details of its prcparation and reactivity with CLL cells and the cells ofother leukaemic patients are described clscwhcre (Sullivan et a / , 1977). The B cell antigen was isolated from human B lymphoblastoid culturcs.…”

Section: Isolation Of Lynrphocyte Sub-populationsmentioning

confidence: 99%

Characterization of Null Cells in Chronic Lymphocytic Leukaemia with B‐Cell Allo‐ and Hetero‐antisera

et al. 1980

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Chronic lymphocytic leukaemia peripheral blood mononuclear cells (CLL-PBMN) were separated into B, T and Null-enriched lymphocyte sub-populations using sequential mouse and sheep red blood cell rosetting depletions on Hypaque-Ficoll gradients. The procedure produced viable cell populations with mean percentage purities of 90, 87 and 75 for B, T and non-rosetting (Null-enriched) sub-populations, respectively. More than 80% of PBMN cells were generally accounted for by mouse and sheep rosetting. The purified lymphocyte sub-populations were examined with a panel of B-cell specific alloantisera obtained from kidney transplant recipients and a rabbit antiserum to B cell antigen isolated from a human B-lymphoblastoid line. The results illustrated that the antigens detected by these sera also have potential as a marker for characterizing the CLL population. Where conventional markers were weak or absent, B cell antigens were readily detected in both fluorescent and cytotoxic tests. The majority of the non-rosetting cells (less than 90%) in CLL followed similar patterns of reactivity to the purified B cells, suggesting they are a subset of B cells. A small residual population (0--5% of PBMN) did not react with the antisera, the significance of which is unknown.

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Section: Isolation Of Lynrphocyte Sub-populationsmentioning

confidence: 99%

Characterization of Null Cells in Chronic Lymphocytic Leukaemia with B‐Cell Allo‐ and Hetero‐antisera

et al. 1980

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“…B-lymphocytes can be identified by heterologous antisera raised against whole 6-cells or B-cell membrane products. Such antisera may vary in their reactions with different cells, Most antisera raised against enzyme-or detergent-solubilized cell membrane preparations seem to detect antigens which are related to the Ia-antigens in rodents ( 5 , IS,22,23,33,34,37). These antisera block the reaction of human alloantisera with 6-lymphocytes as well as the stimulation in mixed lymphocyte culture, and the chemical structure of the antigen is similar to that of mouse la-antigens (6, 25,33,37).…”

mentioning

confidence: 99%

“…These antisera block the reaction of human alloantisera with 6-lymphocytes as well as the stimulation in mixed lymphocyte culture, and the chemical structure of the antigen is similar to that of mouse la-antigens (6, 25,33,37). In man, la-like antigens have been detected not only on B-lymphocytes (normal peripheral blood B-lymphocytes, chronic lymphocytic leukaemia (CLL) lymphocytes, lymphoblastoid B-cell lines) but also on monocytes, blasts in acute lymphocytic leukaemia of non-T, non-B cell type, blast crisis in chronic myelocytic leukaemia (CML) and most cases of acute myeloblastic leukaemia (AML) ( 5 , 32,33,34,37,39). They are also present on immature blast cells in normal bone marrow but not o n more differentiated cells in the myeloid series (23,36).…”

mentioning

confidence: 99%

The Expression of a Human B‐Lymphocyte Antigen on Cells in Different Types of Leukaemia

Turesson

Berntorp

Zettervall

1979

Journal of Internal Medicine

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The expression of a B‐cell antigen on the surface of leukaemic cells from patients with various forms of leukaemia was studied by direct immunofluorescence using a rabbit antiserum against chronic lymphocytic leukaemia (CLL) cells. The antigen was found on normal peripheral blood B‐lymphocytes and on the majority of lymphocytes from patients with CLL. Most normal peripheral blood monocytes and blasts from patients with acute myeloblastic leukaemia, acute myelomonocytic leukaemia or acute lymphoblastic leukaemia of T‐cell type did not carry the antigen but a minority of such cells were weakly stained. In two of three patients with blast transformation of chronic myelocytic leukaemia, a high proportion of the blasts carried the antigen. It was also found on the surface of the hairy cells from two cases of leukaemic reticuloendotheliosis. These cells also carried Fc‐receptors and immunoglobulin with restricted heterogeneity (mu, lambda and alpha, mu, delta, kappa, respectively). The cellular distribution of the B‐cell antigen differs to some extent from that of Ia‐like antigens detected by antisera against purified B‐cell membrane products. The demonstration of the antigen as well as immunoglobulin of a single light chain type on the surface of hairy cells strongly supports the B‐lymphocyte origin of these cells.

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Small lymphocyte T-cell leukemia in the adult

Sullivan

Vera²,

Jerry

et al. 1978

Cancer

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A 49-year-old man is described with morphologic T cell chronic lymphocytic leukemia, whose clinical course, however, progressively deteriorated with central nervous system involvement, resistance to treatment and death within eight months. In addition to widespread organ invasion by leukemic cells there was depression of cellular immunity. The leukemic lymphocytes showed an aberrant response to mitogens, and despite undetectable Ia-like surface antigens were able to stimulate allogeneic cells in the mixed leucocyte reaction. From this and similar cases reviewed herein, it appears that the syndrome of small T-lymphocyte leukemia of the adult is a rapidly aggressive ahd resistant disease with characteristic clinical and laboratory findings.

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Expression of a B-lymphocyte antigen in chronic lymphocytic and other leukemias

Cited by 9 publications

References 29 publications

Characterization of Null Cells in Chronic Lymphocytic Leukaemia with B‐Cell Allo‐ and Hetero‐antisera

Characterization of Null Cells in Chronic Lymphocytic Leukaemia with B‐Cell Allo‐ and Hetero‐antisera

The Expression of a Human B‐Lymphocyte Antigen on Cells in Different Types of Leukaemia

Small lymphocyte T-cell leukemia in the adult

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