Expression of a Chemorepellent Factor, Slit2, in Peripheral Nerve Regeneration

Tanno, Toshihiko; Fujiwara, Ayumi; Takenaka, Shigeo; Kuwamura, Mitsuru; Tsuyama, Shingo

doi:10.1271/bbb.69.2431

Cited by 14 publications

(13 citation statements)

References 13 publications

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“…Two standardized nerve injury models were used to evaluate peripheral nerve regeneration: sciatic nerve crush and sciatic nerve transection (Tanno et al 2005). The sciatic nerves were sectioned for analysis as illustrated in Fig.…”

Section: Resultsmentioning

confidence: 99%

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Dynamic Changes of p27kip1 and Skp2 Expression in Injured Rat Sciatic Nerve

et al. 2007

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S phase kinase-associated protein 2 (Skp2), an F-box protein, is required for the ubiquitination and consequent degradation of p27(kip1). Previous reports have showed that p27(kip1 )played important roles in cell cycle regulation and neurogenesis in the developing central nervous system. But the distribution and function of p27(kip1 )and Skp2 in nervous system lesion and regeneration remains unclear. In this study, we observed that they were expressed mainly in both Schwann cells and axons in adult rat sciatic nerve. Sciatic nerve crush and transection resulted in a significant up-regulation of Skp2 and a down-regulation of p27(kip1). By immunochemistry, we found that in the distal stumps of transected nerve from the end to the edge, the appearance of Skp2 in the edge is coincided with the decrease in p27(kip1) levels. Changes of them were inversely correlated. Results obtained by coimmunoprecipitation and double labeling further showed their interaction in the regenerating process. Thus, these results indicate that p27(kip1 )and Skp2 likely play an important role in peripheral nerve injury and regeneration.

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Section: Resultsmentioning

confidence: 99%

“…The critical feature of crush injuries is that the continuity of basal lamina tubes are protected from injury; in contrast, that of transect is that continuity is completely disrupted (Tanno et al 2005). Thus, the cellular response to nerve injury is also different.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Changes of p27kip1 and Skp2 Expression in Injured Rat Sciatic Nerve

et al. 2007

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“…Among these four classic axon guidance family proteins, Netrin-1 possesses the strongest chemoattractive ability to promote axon extension [15]. Netrins, Slits, Ephrins and Semaphorins continue to be expressed in the adult nervous system and their expression is differentially regulated after nerve injury [16,17,18,19,20], but currently these classic axon guidance molecules have not been extensively studied for their role in nerve regeneration. Interestingly, both Cajal and Sperry studied axon regeneration in order to validate their hypotheses [3,4,21].…”

Section: Introductionmentioning

confidence: 99%

Role of Netrin-1 Signaling in Nerve Regeneration

Dun

Parkinson

2017

IJMS

115

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Netrin-1 was the first axon guidance molecule to be discovered in vertebrates and has a strong chemotropic function for axonal guidance, cell migration, morphogenesis and angiogenesis. It is a secreted axon guidance cue that can trigger attraction by binding to its canonical receptors Deleted in Colorectal Cancer (DCC) and Neogenin or repulsion through binding the DCC/Uncoordinated (Unc5) A–D receptor complex. The crystal structures of Netrin-1/receptor complexes have recently been revealed. These studies have provided a structure based explanation of Netrin-1 bi-functionality. Netrin-1 and its receptor are continuously expressed in the adult nervous system and are differentially regulated after nerve injury. In the adult spinal cord and optic nerve, Netrin-1 has been considered as an inhibitor that contributes to axon regeneration failure after injury. In the peripheral nervous system, Netrin-1 receptors are expressed in Schwann cells, the cell bodies of sensory neurons and the axons of both motor and sensory neurons. Netrin-1 is expressed in Schwann cells and its expression is up-regulated after peripheral nerve transection injury. Recent studies indicated that Netrin-1 plays a positive role in promoting peripheral nerve regeneration, Schwann cell proliferation and migration. Targeting of the Netrin-1 signaling pathway could develop novel therapeutic strategies to promote peripheral nerve regeneration and functional recovery.

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“…Three vertebrate orthologs, slit1, 2, and 3, have been isolated. Each displays overlapping, but distinct, patterns of expression in the vertebrate nervous system [16,17]. Human Slits are candidate ligands for the repulsive guidance receptors of the robo gene family.…”

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confidence: 99%

Expression Patterns of the slit Subfamily mRNA in Canine Malignant Mammary Tumors

Tanno

Tanaka

Sugiura

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. Slit, a secreted protein, functions as a chemorepellent factor in axon guidance and neuronal migration and as an inhibitor in leukocyte chemotaxis. In humans, slit2 protein attracts endothelial cells and promotes tube formation in the tumor angiogenic mechanism. In this study, we cloned a part of the canine slit subfamily and examined the expression of slit subfamily mRNAs in 3 normal canine mammary glands and 11 mammary tumor samples by RT-PCR. The cloned part of the slit gene sequences showed high similarity to those of the human, mouse, and rat. The mRNAs were expressed at low levels in the normal mammary gland. The expression levels of slit1 mRNA were low in both the normal and tumor tissues. In contrast, the expression of slit2 mRNA increased in most of the malignant mammary tumors, and an increase in slit3 mRNA expression was observed in 2 of the malignant mixed tumors. These results suggest that the expression of slit2 plays an important role in tumor angiogenesis in canine mammary gland tumors and that slit2 can be a putative marker for malignancy diagnosis of these tumors. KEY WORDS: canine mammary gland tumor, malignancy, robo, slit, tumor marker.

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Expression of a Chemorepellent Factor, Slit2, in Peripheral Nerve Regeneration

Cited by 14 publications

References 13 publications

Dynamic Changes of p27kip1 and Skp2 Expression in Injured Rat Sciatic Nerve

Dynamic Changes of p27kip1 and Skp2 Expression in Injured Rat Sciatic Nerve

Role of Netrin-1 Signaling in Nerve Regeneration

Expression Patterns of the slit Subfamily mRNA in Canine Malignant Mammary Tumors

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