Expression of a codon-optimized β-glucosidase from Cellulomonas flavigena PR-22 in Saccharomyces cerevisiae for bioethanol production from cellobiose

Ríos-Fránquez, Francisco Javier; González-Bautista, Enrique; Ponce-Noyola, Teresa; Ramos-Valdivia, Ana C.; Poggi‐Varaldo, Héctor M.; García‐Mena, Jaime; Martı́nez, Alfredo

doi:10.1007/s00203-016-1333-2

Cited by 12 publications

(4 citation statements)

References 22 publications

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“…2,3 The enzymatic hydrolysis of lignocellulosic biomass into simple sugars that can be fermented into biofuel, requires a complete set of enzymes that includes cellulase, hemicellulases and ligninases. 4 To depolymerize the lignocellulosic plant biomass, these enzymes act synergistically and specically and convert it into simple fermentable sugars for biofuel production at industrial scale. 5 Based on their mode of action, the cellulases are divided into three groups: b-glucosidases, cellobiohydrolase and endoglucanase.…”

Section: Introductionmentioning

confidence: 99%

Efficient biomass saccharification using a novel cellobiohydrolase from Clostridium clariflavum for utilization in biofuel industry

et al. 2021

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Section: Introductionmentioning

confidence: 99%

Efficient biomass saccharification using a novel cellobiohydrolase from Clostridium clariflavum for utilization in biofuel industry

et al. 2021

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“…These results indicated that Micrococcales plays an important role in the resistance of functional microbial community under CCR. We speculated that the up-regulated glucose tolerant β-glucosidases could induce cellulase formation by sophorose produced by transglycosylation [ 54 , 55 ]. In Ríos-Fránquez’s [ 54 ] study, Cellulomonas flavigena PR-22 showed more resistance to catabolic repression than its predecessor PN-120 strain, and the β-glucosidase produced by PR-22 exhibited more glucose tolerance.…”

Section: Discussionmentioning

confidence: 99%

“…We speculated that the up-regulated glucose tolerant β-glucosidases could induce cellulase formation by sophorose produced by transglycosylation [ 54 , 55 ]. In Ríos-Fránquez’s [ 54 ] study, Cellulomonas flavigena PR-22 showed more resistance to catabolic repression than its predecessor PN-120 strain, and the β-glucosidase produced by PR-22 exhibited more glucose tolerance. Hiida Rodriguez et al [ 55 ] reported that β-glucosidase could play a role in the formation of a natural cellulase inducer during the growth of Cellulomonas on sugarcane bagasse [ 55 ] we speculated that the cellulase inducer was a transglycosylation product of β-glucosidase.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of differential expression of β-glucosidase genes in functional microbial communities in response to carbon catabolite repression

Zhang

Chen

et al. 2022

Biotechnol Biofuels

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Background β-Glucosidase is the rate-limiting enzyme of cellulose degradation. It has been stipulated and established that β-glucosidase-producing microbial communities differentially regulate the expression of glucose/non-glucose tolerant β-glucosidase genes. However, it is still unknown if this differential expression of functional microbial community happens accidentally or as a general regulatory mechanism, and of what biological significance it has. To investigate the composition and function of microbial communities and how they respond to different carbon metabolism pressures and the transcriptional regulation of functional genes, the different carbon metabolism pressure was constructed by setting up the static chamber during composting. Results The composition and function of functional microbial communities demonstrated different behaviors under the carbon metabolism pressure. Functional microbial community up-regulated glucose tolerant β-glucosidase genes expression to maintain the carbon metabolism rate by enhancing the transglycosylation activity of β-glucosidase to compensate for the decrease of hydrolysis activity under carbon catabolite repression (CCR). Micrococcales play a vital role in the resistance of functional microbial community under CCR. The transcription regulation of GH1 family β-glucosidase genes from Proteobacteria showed more obvious inhibition than other phyla under CCR. Conclusion Microbial functional communities differentially regulate the expression of glucose/non-glucose tolerant β-glucosidase genes under CCR, which is a general regulatory mechanism, not accidental. Furthermore, the differentially expressed β-glucosidase gene exhibited species characteristics at the phylogenetic level.

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“…Despite major efforts to create recombinant strains of S. cerevisiae capable of utilizing cellobiose via the expression of heterologous genes encoding β-glucosidase, the ethanol yield and productivity of the resultant yeast strains still need improvement. Rios-Franquez et al [14] have employed a codon-optimized β-glucosidase from Cellulomonas flavigena to produce ethanol from cellobiose. This S. cerevisiae recombinant strain displayed an ethanol yield reaching 76% of the theoretical value.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Heterologous secretory expression of β-glucosidase from Thermoascus aurantiacus in industrial Saccharomyces cerevisiae strains

et al. 2019

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The use of plant biomass for biofuel production will require efficient utilization of the sugars in lignocellulose, primarily cellobiose, because it is the major soluble by-product of cellulose and acts as a strong inhibitor, especially for cellobiohydrolase, which plays a key role in cellulose hydrolysis. Commonly used ethanologenic yeast Saccharomyces cerevisiae is unable to utilize cellobiose; accordingly, genetic engineering efforts have been made to transfer β-glucosidase genes enabling cellobiose utilization. Nonetheless, laboratory yeast strains have been employed for most of this research, and such strains may be difficult to use in industrial processes because of their generally weaker resistance to stressors and worse fermenting abilities. The purpose of this study was to engineer industrial yeast strains to ferment cellobiose after stable integration of tabgl1 gene that encodes a β-glucosidase from Thermoascus aurantiacus (TaBgl1). The recombinant S. cerevisiae strains obtained in this study secrete TaBgl1, which can hydrolyze cellobiose and produce ethanol. This study clearly indicates that the extent of glycosylation of secreted TaBgl1 depends from the yeast strains used and is greatly influenced by carbon sources (cellobiose or glucose). The recombinant yeast strains showed high osmotolerance and resistance to various concentrations of ethanol and furfural and to high temperatures. Therefore, these yeast strains are suitable for ethanol production processes with saccharified lignocellulose.

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Expression of a codon-optimized β-glucosidase from Cellulomonas flavigena PR-22 in Saccharomyces cerevisiae for bioethanol production from cellobiose

Cited by 12 publications

References 22 publications

Efficient biomass saccharification using a novel cellobiohydrolase from Clostridium clariflavum for utilization in biofuel industry

Efficient biomass saccharification using a novel cellobiohydrolase from Clostridium clariflavum for utilization in biofuel industry

Mechanism of differential expression of β-glucosidase genes in functional microbial communities in response to carbon catabolite repression

Heterologous secretory expression of β-glucosidase from Thermoascus aurantiacus in industrial Saccharomyces cerevisiae strains

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