Expression of a cystatin transgene can confer resistance to root lesion nematodes in Lilium longiflorum cv. ‘Nellie White’

Vieira, Paulo; Wantoch, Sarah; Lilley, Catherine J.; Chitwood, David J.; Atkinson, H. J.; Kamo, Kathryn

doi:10.1007/s11248-014-9848-2

Cited by 48 publications

(24 citation statements)

References 47 publications

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“…Previously, Vieira et al . () identified in the transcriptome of P. penetrans a highly abundant transcript encoding for a FAR gene. Along with the in silico analyses performed in this study, three different FAR members of this family were identified in P. penetrans , and a molecular characterization of the Pp‐far‐1 gene is provided herein.…”

Section: Introductionmentioning

confidence: 99%

Characterization and silencing of the fatty acid‐ and retinol‐binding Pp‐far‐1 gene in Pratylenchus penetrans

2017

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Fatty acid‐ and retinol‐binding proteins (FARs) are unique to nematodes, and are implicated in a wide range of metabolic and parasitic related functions. Along with the in silico analyses performed in this study, three different FAR members of this family were identified in Pratylenchus penetrans. The cDNA corresponding to the fatty acid‐ and retinol‐binding Pp‐far‐1 gene was cloned and herein characterized at the molecular level for the first time for this genus. The translated 185 amino acid sequence of P. penetrans FAR‐1 sequence, with a predicted molecular weight of 20.82 kDa and a pI of 5.49, shares highest sequence identity to FARs of other migratory nematodes of the Pratylenchidae family (90% to P. vulnus and 80% to Radopholus similis). In situ hybridization localizes Pp‐far‐1 transcripts in the hypodermis of the nematode. RT‐qPCR detected Pp‐far‐1 transcripts for all nematode developmental stages, with highest expression levels found in juveniles, adult females and adult males, respectively. Pp‐far‐1 is also highly expressed during infection and establishment of P. penetrans in roots of different host plants, such as corn, lily and soybean. The importance of Pp‐far‐1 was studied by in planta RNA interference (RNAi) assays using stable soybean hairy root lines. Targeting Pp‐far‐1 decreased expression of the nematode Pp‐far‐1 and significantly reduced the reproduction of nematodes, with a total of between 44% and 70% fewer nematodes in comparison to the average number of nematodes counted for control lines. The results indicate that suppressing the expression levels of Pp‐far‐1 can act as an effective target gene to control P. penetrans.

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Section: Introductionmentioning

confidence: 99%

Characterization and silencing of the fatty acid‐ and retinol‐binding Pp‐far‐1 gene in Pratylenchus penetrans

2017

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“…Nellie White exhibited comparatively high resistant to an endoparasite, Pratylenchus penetrans causing RLN (root lesion disease). OcI Δ D86 expression inhibited parasitic effects of Pratylenchus by 75% in transgenic plants as compared to their wild relatives (Vieira et al, 2015; Table 4). …”

Section: Abiotic and Biotic Stress Tolerancementioning

confidence: 99%

Biotechnological Advancements for Improving Floral Attributes in Ornamental Plants

et al. 2017

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Developing new ornamental cultivars with improved floral attributes is a major goal in floriculture. Biotechnological approach together with classical breeding methods has been used to modify floral color, appearance as well as for increasing disease resistance. Transgenic strategies possess immense potential to produce novel flower phenotypes that are not found in nature. Adoption of Genetic engineering has supported the idea of floral trait modification. Ornamental plant attributes like floral color, fragrance, disease resistance, and vase life can be improved by means of genetic manipulation. Therefore, we witness transgenic plant varieties of high aesthetic and commercial value. This review focuses on biotechnological advancements in manipulating key floral traits that contribute in development of diverse ornamental plant lines. Data clearly reveals that regulation of biosynthetic pathways related to characteristics like pigment production, flower morphology and fragrance is both possible and predictable. In spite of their great significance, small number of genetically engineered varieties of ornamental plants has been field tested. Today, novel flower colors production is regarded as chief commercial benefit obtained from transgenic plants. But certain other floral traits are much more important and have high commercial potential. Other than achievements such as novel architecture, modified flower color, etc., very few reports are available regarding successful transformation of other valuable horticultural characteristics. Our review also summarized biotechnological efforts related to enhancement of fragrance and induction of early flowering along with changes in floral anatomy and morphology.

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“…This rice cystatin has also shown good control in lily ( Lilium longiflorum cv. ‘Nellie White’) against lesion nematode ( P. penetrans ) (Vieira et al, 2015). …”

Section: Use Of Proteinase Inhibitor Coding Genesmentioning

confidence: 99%

Transgenic Strategies for Enhancement of Nematode Resistance in Plants

et al. 2017

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Plant parasitic nematodes (PPNs) are obligate biotrophic parasites causing serious damage and reduction in crop yields. Several economically important genera parasitize various crop plants. The root-knot, root lesion, and cyst nematodes are the three most economically damaging genera of PPNs on crops within the family Heteroderidae. It is very important to devise various management strategies against PPNs in economically important crop plants. Genetic engineering has proven a promising tool for the development of biotic and abiotic stress tolerance in crop plants. Additionally, the genetic engineering leading to transgenic plants harboring nematode resistance genes has demonstrated its significance in the field of plant nematology. Here, we have discussed the use of genetic engineering for the development of nematode resistance in plants. This review article also provides a detailed account of transgenic strategies for the resistance against PPNs. The strategies include natural resistance genes, cloning of proteinase inhibitor coding genes, anti-nematodal proteins and use of RNA interference to suppress nematode effectors. Furthermore, the manipulation of expression levels of genes induced and suppressed by nematodes has also been suggested as an innovative approach for inducing nematode resistance in plants. The information in this article will provide an array of possibilities to engineer resistance against PPNs in different crop plants.

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Expression of a cystatin transgene can confer resistance to root lesion nematodes in Lilium longiflorum cv. ‘Nellie White’

Cited by 48 publications

References 47 publications

Characterization and silencing of the fatty acid‐ and retinol‐binding Pp‐far‐1 gene in Pratylenchus penetrans

Characterization and silencing of the fatty acid‐ and retinol‐binding Pp‐far‐1 gene in Pratylenchus penetrans

Biotechnological Advancements for Improving Floral Attributes in Ornamental Plants

Transgenic Strategies for Enhancement of Nematode Resistance in Plants

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