Expression of a peptide epitope of the colonic mucin MUC2 in precursor lesions to gastric carcinoma

Expression of fully and under-glycosylated forms of MUC1 mucin in gastric carcinoma

“…Our study demonstrates therefore that type I intestinal metaplasia of the gastric mucosa is accompanied by a modification in the pattern of mucins that follows a true intestinal phenotype. Similar findings were reported by Ho et al (1993) and Filipe et al (1996). The fine regulatory mechanisms underlying this modification need to be clarified in future studies.…”

“…In 18 cases, gastric mucosa adjacent to the carcinomas was also evaluated. Detection and classification of intestinal metaplasia was performed after staining the sections with Alcian blue pH 2.5/periodic acid Schiff and Alcian blue pH 2.5/high iron diamine (Filipe et al, 1996).…”

“…Particularly interesting is the incomplete carbohydrate biosynthesis, which causes aberrant exposure of determinants within the mucin protein core (Burchell et al, 1987;Girling et al, 1989). In contrast with this, the mucin core proteins are similar in normal and neoplastic cells, although up-regulation (Girling et al, 1989;Ho et al, 1993), down-regulation (Ho et al, 1993) and aberrant expression (Ho et al, 1993) may also occur in cancer cells and in pre-neoplastic lesions (Filipe et al, 1996;Ho et al, 1993).…”

Expression ofMUC2 gene in gastric regenerative, metaplastic, and neoplastic epithelia

“…The remaining Nterminal and C-terminal regions are rich in Cys, and these Cys residues are considered to be important for the joining of MUC2 monomers into large polymers with high intrinsic viscosity (1). It has previously been reported that MUC2 mucin core protein (MUC2 protein) is expressed in intestinal metaplasia and carcinoma of the stomach, but not in its normal epithelium (2,3), suggesting that it may be a new marker for intestinal metaplasia and may play an important role as a component of the mucous gel overlying the metaplastic epithelium in the stomach. On the other hand, it is considered that chronic active gastritis induced by Helicobacter pylori (H. pylori) could result in atrophic gastritis concurring with extensive intestinal metaplasia, in the latter of which H. pylori is not usually found (4,5).…”

Immunohistochemical Study of the Expression of MUC6 Mucin and Co-expression of Other Secreted Mucins (MUC5AC and MUC2) in Human Gastric Carcinomas

“…It includes mucins normally expressed in the gastric mucosa (MUC1, MUC5AC and MUC6) and de novo expression of the intestinal mucin MUC2 (Ho et al 1993(Ho et al ,1995Carrato et al 1994;Ho et al 1995;Sakamoto et al 1997;Reis et al 1998a;Baldus et al 1998;Utsunomiya et al 1998). Furthermore, decreased expression of "gastric" mucins and de novo expression of MUC2 have also been described in intestinal metaplasia (Ho et al 1995;Filipe et al 1996;Reis et al 1998bReis et al ,1999, a precancerous lesion of gastric carcinomas (Correa 1988(Correa ,1992Filipe et al 1994). These observations suggest that the repertoire of mucins synthesized by gastric carcinoma cells is tightly associated with their differentiation program.…”