Expression of a type II collagen gene in the zebrafish embryonic axis

Yan, Yi‐Lin; Hatta, Kohei; Riggleman, Bob; Postlethwait, John H.

doi:10.1002/aja.1002030308

Cited by 213 publications

(166 citation statements)

References 71 publications

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“…In this case, the stomochord and notochord share only the genetic regulatory machinery for the hydrostatic skeleton and patterning of the nervous system. It is notable that the hedgehog and ColA-positive cells are also found in the anterior endoderm in chordates 35,36 , which may represent the homologue of the hedgehog-positive endoderm cells of hemichordates. In either scenario, the genetic linkage between the hedgehog secreting cells and brachyury expression was established in the chordate ancestors.…”

Section: Discussionmentioning

confidence: 99%

Hemichordate neurulation and the origin of the neural tube

Miyamoto

Wada

2013

Nat Commun

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The origin of the body plan of our own phylum, Chordata, is one of the most fascinating questions in evolutionary biology. Yet, after more than a century of debate, the evolutionary origins of the neural tube and notochord remain unclear. Here we examine the development of the collar nerve cord in the hemichordate Balanoglossus simodensis and find shared gene expression patterns between hemichordate and chordate neurulation. Moreover, we show that the dorsal endoderm of the buccal tube and the stomochord expresses Hedgehog RNA, and it seems likely that collar cord cells can receive the signal. Our data suggest that the endoderm functions as an organizer to pattern the overlying collar cord, similar to the relationship between the notochord and neural tube in chordates. We propose that the origin of the core genetic mechanisms for the development of the notochord and the neural tube date back to the last common deuterostome ancestor.

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Section: Discussionmentioning

confidence: 99%

Hemichordate neurulation and the origin of the neural tube

Miyamoto

Wada

2013

Nat Commun

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“…Mutations in shh (sonic-you) only partially affected floor plate differentiation and revealed mechanistic differences in the establishment of the medial and the lateral rows of the floor plate (Schauerte et al, 1998;Odenthal et al, 2000). Lateral (LFP) and medial floor plate (MFP) can be distinguished by gene expression patterns, with fkh4 (pintal-lavis; Odenthal and Nü sslein-Volhard, 1998) and foxA2 (axial/HNF3␤; Strä hle et al, 1993) expressed in both MFP and LFP, and shh (Krauss et al, 1993), netrin1 (Strä hle et al, 1997a), and col2␣1 (Yan et al, 1995) expressed only in the MFP. The nk2.2 gene is expressed in the LFP only (Barth and Wilson, 1995).…”

Section: Introductionmentioning

confidence: 99%

Cyclops‐independent floor plate differentiation in zebrafish embryos

Albert

Müller

Fischer

et al. 2002

Developmental Dynamics

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In zebrafish, development of the ventral neural tube depends on the Nodal-related signal Cyclops (Cyc). One-day-old cyc mutant embryos lack the medial floor plate (MFP). We show here that cells expressing MFP marker genes differentiate gradually in cyc mutant embryos in a delayed manner during the second day of development. This late differentiation is restricted to the hindbrain and spinal cord and depends on an intact Hedgehog (Hh) signalling pathway. Cells expressing MFP marker genes in cyc mutant embryos appear to be derived from lateral floor plate (LFP) cells as they coexpress LFP and MFP marker genes. This finding suggests that the correct temporal development of the MFP is required for the distinction of LFP and MFP cells in wild-type embryos. Developmental Dynamics 226:59 -66, 2003.

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“…The mutant embryos presented an ovoid shape with a widening of the notochord at the tail bud stage and, later during development, a tail wound-up on the back (39). Thus to better characterize the morphological and structural changes occurring in the zIFT46 morphants, we used in situ hybridization to analyze the expression of the zebrafish Col2a1 gene (zCol2a1), a characteristic marker of the differentiating notochord (40). At 13 hpf, the zCol2a1 hybridization signal clearly reveals enlargement of the notochord in zIFT46-MO-injected embryos compared with wild-type (Fig.…”

Section: Profilermentioning

confidence: 99%

Knockdown of the Intraflagellar Transport Protein IFT46 Stimulates Selective Gene Expression in Mouse Chondrocytes and Affects Early Development in Zebrafish

Gouttenoire¹,

Valcourt²,

Bougault³

et al. 2007

Journal of Biological Chemistry

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Bone morphogenetic proteins (BMPs) act as multifunctional regulators in morphogenesis during development. In particular they play a determinant role in the formation of cartilage molds and their replacement by bone during endochondral ossification. In cell culture, BMP-2 favors chondrogenic expression and promotes hypertrophic maturation of chondrocytes. In mouse chondrocytes we have identified a BMP-2-sensitive gene encoding a protein of 301 amino acids. This protein, named mIFT46, is the mouse ortholog of recently identified Caenorhabditis elegans and Chlamydomonas reinhardtii intraflagellar transport (IFT) proteins. After generation of a polyclonal antibody against mIFT46, we showed for the first time that the endogenous protein is located in the primary cilium of chondrocytes. We also found that mIFT46 is preferentially expressed in early hypertrophic chondrocytes located in the growth plate. Additionally, mIFT46 knockdown by small interfering RNA oligonucleotides in cultured chondrocytes specifically stimulated the expression of several genes related to skeletogenesis. Furthermore, Northern blotting analysis indicated that mIFT46 is also expressed before chondrogenesis in embryonic mouse development, suggesting that the role of mIFT46 might not be restricted to cartilage. To explore the role of IFT46 during early development, we injected antisense morpholino oligonucleotides in Danio rerio embryos to reduce zebrafish IFT46 protein (zIFT46) synthesis. Dramatic defects in embryonic development such as a dorsalization and a tail duplication were observed. Thus our results taken together indicate that the ciliary protein IFT46 has a specific function in chondrocytes and is also essential for normal development of vertebrates.In vertebrates endochondral bone formation involves first the formation of cartilage primordia, followed by its replacement by bone. This process is associated with several changes in the differentiation status of chondrocytes in the growth plate and results in a modification of the extracellular matrix composition. The cartilage matrix contains predominantly type II collagen. During endochondral ossification, chondrocytes enter a process of maturation characterized by cellular hypertrophy, a decrease in type II collagen expression, and the onset in type X collagen expression. After full differentiation of hypertrophic chondrocytes the cartilage matrix calcifies. Ossification begins with the invasion of the calcified cartilage by blood capillaries. This neo-vascularization of the growth plate is concomitant with apoptosis of most hypertrophic chondrocytes. The resulting cartilage matrix provides a scaffold for osteoblasts, which invade the cartilage mold, along with blood vessels, so laying down a bone matrix characterized by the presence of osteocalcin (reviewed in Ref.

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Expression of a type II collagen gene in the zebrafish embryonic axis

Cited by 213 publications

References 71 publications

Hemichordate neurulation and the origin of the neural tube

Hemichordate neurulation and the origin of the neural tube

Cyclops‐independent floor plate differentiation in zebrafish embryos

Knockdown of the Intraflagellar Transport Protein IFT46 Stimulates Selective Gene Expression in Mouse Chondrocytes and Affects Early Development in Zebrafish

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