Expression of adipokines and adipocytokines by epidural adipose tissue in cauda equina syndrome in dogs

Leisengang, Stephan; Gluding, D; Hörster, Julia; Peek, Verena; Ott, Daniela; Rummel, Christoph; Schmidt, Martin J.

doi:10.1111/jvim.16483

Cited by 3 publications

(1 citation statement)

References 72 publications

(159 reference statements)

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“…The research conducted by Yang indicated that CXCL8 and CXCL11 might enhance the expression of inflammatory cytokines, promote the apoptosis of chondrocytes and inhibit the proliferation by affecting the JAK-STAT signaling pathway. The results suggest that JAK/STAT signaling pathway plays an important role in the pathogenesis of OA [ 52 ]. The PI3K/Akt signaling pathway is a very classical signaling pathway that can regulate cell growth, proliferation, differentiation, and other life processes.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of Wenshen Xuanbi Decoction in the treatment of osteoarthritis based on network pharmacology and experimental verification

You,

Song,

Liu

et al. 2024

Korean J Physiol Pharmacol

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To investigate the mechanism of Wenshen Xuanbi Decoction (WSXB) in treating osteoarthritis (OA) via network pharmacology, bioinformatics analysis, and experimental verification. The active components and prediction targets of WSXB were obtained from the TCMSP database and Swiss Target Prediction website, respectively. OA-related genes were retrieved from GeneCards and OMIM databases. Protein-protein interaction and functional enrichment analyses were performed, resulting in the construction of the Herb-Component-Target network. In addition, differential genes of OA were obtained from the GEO database to verify the potential mechanism of WSXB in OA treatment. Subsequently, potential active components were subjected to molecular verification with the hub targets. Finally, we selected the most crucial hub targets and pathways for experimental verification in vitro. The active components in the study included quercetin, linolenic acid, methyl linoleate, isobergapten, and beta-sitosterol. AKT1, tumor necrosis factor (TNF), interleukin (IL)-6, GAPDH, and CTNNB1 were identified as the most crucial hub targets. Molecular docking revealed that the active components and hub targets exhibited strong binding energy. Experimental verification demonstrated that the mRNA and protein expression levels of IL-6, IL-17, and TNF in the WSXB group were lower than those in the KOA group (p < 0.05). WSXB exhibits a chondroprotective effect on OA and delays disease progression. The mechanism is potentially related to the suppression of IL-17 and TNF signaling pathways and the down-regulation of IL-6.

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Section: Discussionmentioning

confidence: 99%

Mechanism of Wenshen Xuanbi Decoction in the treatment of osteoarthritis based on network pharmacology and experimental verification

You,

Song,

Liu

et al. 2024

Korean J Physiol Pharmacol

View full text Add to dashboard Cite

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Expression of adipokines and adipocytokines by epidural adipose tissue in cauda equina syndrome in dogs

Leisengang

Gluding

Hörster

et al. 2022

Veterinary Internal Medicne

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Background: Compression of epidural adipose tissue (EAT) within the scope of cauda equina syndrome (CES) could lead to an enhanced expression of inflammatory mediators, possibly contributing to pain amplification in dogs. Objectives:To analyze expression of inflammatory adipo(-cyto)kines within the EAT of dogs with CES.Animals: Client-owned dogs: 15 dogs with CES and 9 dogs euthanized for unrelated medical reasons (controls).Methods: Prospective, experimental study. Epidural adipose tissue and subcutaneous adipose tissue were collected during dorsal laminectomy and used for real-time quantitative polymerase chain reaction. Tissue explants were cultured for measurements of inflammation-induced release of cytokines.Results: Results show a CES-associated upregulation of the cytokines tumor necrosis

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Effects of Omega-3 Polyunsaturated Fatty Acids on the Formation of Adipokines, Cytokines, and Oxylipins in Retroperitoneal Adipose Tissue of Mice

Wenderoth,

Feldotto,

Hernandez

et al. 2024

IJMS

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Oxylipins and specialized pro-resolving lipid mediators (SPMs) derived from polyunsaturated fatty acids (PUFAs) are mediators that coordinate an active process of inflammation resolution. While these mediators have potential as circulating biomarkers for several disease states with inflammatory components, the source of plasma oxylipins/SPMs remains a matter of debate but may involve white adipose tissue (WAT). Here, we aimed to investigate to what extent high or low omega (n)-3 PUFA enrichment affects the production of cytokines and adipokines (RT-PCR), as well as oxylipins/SPMs (liquid chromatography–tandem mass spectrometry) in the WAT of mice during lipopolysaccharide (LPS)-induced systemic inflammation (intraperitoneal injection, 2.5 mg/kg, 24 h). For this purpose, n-3 PUFA genetically enriched mice (FAT-1), which endogenously synthesize n-3 PUFAs, were compared to wild-type mice (WT) and combined with n-3 PUFA-sufficient or deficient diets. LPS-induced systemic inflammation resulted in the decreased expression of most adipokines and interleukin-6 in WAT, whereas the n-3-sufficient diet increased them compared to the deficient diet. The n-6 PUFA arachidonic acid was decreased in WAT of FAT-1 mice, while n-3 derived PUFAs (eicosapentaenoic acid, docosahexaenoic acid) and their metabolites (oxylipins/SPMs) were increased in WAT by genetic and nutritional n-3 enrichment. Several oxylipins/SPMs were increased by LPS treatment in WAT compared to PBS-treated controls in genetically n-3 enriched FAT-1 mice. Overall, we show that WAT may significantly contribute to circulating oxylipin production. Moreover, n-3-sufficient or n-3-deficient diets alter adipokine production. The precise interplay between cytokines, adipokines, and oxylipins remains to be further investigated.

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Expression of adipokines and adipocytokines by epidural adipose tissue in cauda equina syndrome in dogs

Cited by 3 publications

References 72 publications

Mechanism of Wenshen Xuanbi Decoction in the treatment of osteoarthritis based on network pharmacology and experimental verification

Mechanism of Wenshen Xuanbi Decoction in the treatment of osteoarthritis based on network pharmacology and experimental verification

Expression of adipokines and adipocytokines by epidural adipose tissue in cauda equina syndrome in dogs

Effects of Omega-3 Polyunsaturated Fatty Acids on the Formation of Adipokines, Cytokines, and Oxylipins in Retroperitoneal Adipose Tissue of Mice

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