Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp. USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products

Chee, Jiun-Yee; Lau, Nyok-Sean; Samian, Mohammed Razip; Tsuge, Takeharu; Sudesh, Kumar

doi:10.1111/j.1365-2672.2011.05189.x

Cited by 22 publications

(10 citation statements)

References 51 publications

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“…Some exceptions were observed; in literature it was reported that several class I PhaCs may produce scl-mcl-PHA. PhaCAc is considered as one of the most successful enzymes used for scl-mcl-PHA production, while Class IV PHA synthases from Bacillus cereus type (IVc) [Chee et al, 2012;Mezzolla and Poltronieri, 2018].…”

Section: Introductionmentioning

confidence: 99%

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Yasin¹,

Al-Mayaly²

2021

J. Ecol. Eng.

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Polyhydroxyalkanoates (PHAs) have gained much attention as biodegradable polymers, many efforts are being made to minimize the cost of PHAs by finding cheap carbon source depending on the type of microorganism and fermentation conditions. The aims of this study were to evaluate the effects of different glucose concentrations and other important conditions on the PHA production by Bacillus cereus isolated from soil. Polyhydroxyalkanoates PHAs accumulated by soil microorganisms were examined by screening the isolated bacteria using Sudan B Black and Nile Blue staining process. A Gram positive strain was identified using the 16s rRNA gene, deposited in the NCBI GenBank sequence database. Different growth conditions (favorite glucose concentrations 1-8 % (w/v), temperatures and pH) were tested and the growth parameters (sugar consumption, cell counting and Cell Dry Weight CDW) were studied. The extracted polymers were analyzed and characterized using an FTIR spectrophotometer followed by a GC-MS analysis. The pure bacterial strain isolated from soil was deposited in the NCBI GenBank database B. cereus strain ARY73, which showed significant black colored granules (or dark blue) using Sudan B Black stain, it also showed positive to Nile blue A as a high indicator stain for PHA accumulation. B. cereus ARY73 showed high production of PHA using (w/v): 2% glucose and 1% nitrogen source at 35 °C and pH7 yields 79% per Cell Dry Weight and 96 h of incubation. The extracted polymers were analyzed and characterized using an FTIR spectrophotometer confirming the PHA structure. The FTIR spectrophotometer, followed by a GC-MS analysis indicated the Scl-co-mcl PHA structure. This research demonstrates that the isolated strain B. cereus ARY73 was a good candidate for PHA production with a better quality for use in biomedical and other applications. The use of biopolymer in soil, enhanced the accumulation of the microorganisms (such as bacteria) capable of degrading biopolymer or biodegradation by-products yields by other species which were isolated in this study and demonstrated their efficiency in producing biopolymers.

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Section: Introductionmentioning

confidence: 99%

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Yasin¹,

Al-Mayaly²

2021

J. Ecol. Eng.

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“…Microbial synthesis of PHB from the C1 resource is a potential way to address the issue (Chee et al . ).…”

Section: Introductionmentioning

confidence: 97%

Two‐stage fermentation optimization for poly‐3‐hydroxybutyrate production from methanol by a new Methylobacterium isolate from oil fields

Wang

Tan

et al. 2019

J Appl Microbiol

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Aims:We aimed to explore a new Methylobacterium isolate to produce polyhydroxybutyrate (PHB) by using methanol as a sole carbon resource and improve PHB production. Methods and Results: A new PHB-producing isolate (Methylobacterium sp. 1805) was obtained from oil fields by using methanol as a sole carbon source. The fermentation situation of PHB production was further optimized by using Box-Behnken response surface methodology (RSM). Before optimization, the cell biomass was 0Á6 g l À1 after 3-day culture and 0Á3 g l À1 PHB was produced after 5-day methanol-inducing stage. The RSM growth medium was optimized as 15 g l À1 glycerol, 10 g l À1 beef extract and 0Á65 g l À1 MgSO 4 Á7H 2 O. The RSM methanol-inducing medium was optimized as 0Á65 g l À1 MgSO 4 (metal ions), 20 mmol l À1 PBS pH 6Á5 and final 2% methanol (v/v). The biomass and PHB production reached 1Á0 and 0Á55 g l À1 after 3-day culture, respectively. The PHB yield increased by about 80% when compared with before optimization. Conclusions: The optimization of a two-stage fermentation process improved PHB production from methanol by using Methylobacterium sp. 1805. Significance and Impact of the Study: A new Methylobacterium isolate was isolated and produced high-level PHB by using methanol as a sole carbon resource. The bacteria will provide a potential tool for C1 resource in producing PHB.

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“…(Chee et al, 2010). Plastic degradation by microbes due to the activity of certain enzymes that cause cleavage of the polymer chains into monomers and oligomers.…”

Section: Introductionmentioning

confidence: 99%

Screening Of Plastic Degrading Bacteria from Dumped Soil Area

Jumaah¹

2017

IOSR JESTFT

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Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp. USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products

Cited by 22 publications

References 51 publications

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Two‐stage fermentation optimization for poly‐3‐hydroxybutyrate production from methanol by a new Methylobacterium isolate from oil fields

Screening Of Plastic Degrading Bacteria from Dumped Soil Area

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