Expression of afibrobacter succinogenes 1,3-1,4-β-glucanase in Potato (Solanum tuberosum)

Feeding value in broiler chicken diets of a potato expressing a β-glucanase gene from Fibrobacter succinogenes

“…Chicks on the Avizyme-supplemented CDC Silky-based diet also had lower (P < 0.05) feed conversion ratios than those fed the control diet (1.56 compared to 2.05). This improvement in feed efficiency and (Armstrong et al 2002). The transformed potato provided 30 and 60 Units of β-glucanase per kg diet, when included at 0.6 and 1.2 g kg -1 , respectively.…”

“…Supplementing chicken diets with commercial β-glucanases hydrolyzes the β-glucans, thereby decreasing intestinal viscosity and improving digestive parameters (Almirall et al 1993). Researchers at Agriculture and AgriFood Canada, Lethbridge, AB, developed a potato cultivar capable of expressing 1,3-1,4-β-D-glucan 4-glucanohydrolase from Fibrobacter succinogenes (Armstrong et al 2002). The enzyme can be found in concentrations as high as 0.05% of the fresh tuber weight with a specific activity of 3013 Units mg -1 glucanase.…”

“…Desiree) -either untransformed or transformed. The transformed potato expressed a β-glucanase gene from Fibrobacter succinogenes (Armstrong et al 2002). Potato was substituted for equal amounts of barley in the diets.…”

“…The transformed potato used in this study expressed the Fibrobacter succinogenes β-glucanase at a concentration of 10 Units g -1 of fresh tuber tissue and a specific activity of 3013 Units mg -1 glucanase (Armstrong et al 2002). Thus, including the transformed potato at 0.6 and 1.2 g kg -1 provided 30 and 60 Units kg -1 in the diets.…”

“…Hessleman et al (1981) estimated that β-glucanase supplementation of 5 Units kg -1 of barley-based diets was necessary to achieve acceptable growth rates in chickens. The cost of producing 60 Units of glucanase from the Fibrobacter succinogenes β-glucanase-expressing potato is approximately equivalent to the cost of acquiring 5 Units of glucanase from conventional sources such as Avizyme 1100 (Armstrong et al 2002).…”