Expression of an epitopic region of AspfI, an allergen/antigen/cytotoxin of Aspergillus fumigatus

Sarma, Potukuchi Venkata Gurunadha Krishna; Purkayastha, Swagata; Madan, Taruna; Sarma, P. Usha

doi:10.1016/s0165-2478(99)00140-6

Cited by 9 publications

(8 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, Kurup et al showed that three peptides from the C terminus of Asp f 1 significantly inhibited IgE binding of Asp f 1 (inhibition ranged from 80 to 100%) (18). In an earlier study, a recombinant N-terminal fragment of Asp f 1 (8 to 45 amino acid residues), which comprised the immunodominant region of the present study, showed binding to specific IgG and IgE antibodies in ABPA patients by immunoblotting and ELISA (29).…”

Section: Discussionsupporting

confidence: 45%

“…The National Center for Biotechnology Information protein BLAST tool for short sequences in fungi showed that P1 has complete sequence similarity only to Asp f 1 and related ribotoxins of other Aspergillus species (E value, 1e-04) and partial similarity to elongation factor 2 of various species of Saccharomyces and Candida (only 7 out of 11 amino acid residues were identical, with an E value of 4.3). The comparable sensitivity of P1 to that of SDA may be due to the presence of homologous IgG binding epitopes in six immunodominant allergens and antigens (24,29,55,66,77, and 88 kDa) and homologous IgE binding epitopes in three immunodominant allergens and antigens (27,34, and 43 kDa) besides Asp f 1 (28).…”

Section: Discussionmentioning

confidence: 94%

See 1 more Smart Citation

Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen ofAspergillus fumigatus, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis

Madan

Priyadarsiny

Vaid

et al. 2004

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

Allergic bronchopulmonary aspergillosis (ABPA) is an immunologically complex allergic disorder caused by the fungal pathogen Aspergillus fumigatus. Elevated levels of total immunoglobulin E (IgE), specific IgE, and IgG antibodies in sera are important immunodiagnostic criteria for ABPA. International reference standards or standardized immunodiagnostic assays are not available due to a lack of well-defined diagnostic antigens. The present study was carried out to identify and evaluate the immunodiagnostic relevance of synthetic epitopic peptides of Asp f 1, a major allergen, antigen, or cytotoxin of A. fumigatus. Five overlapping peptides were synthesized from the N terminus of Asp f 1, one of the potential immunodominant regions predicted by algorithmic programs. The 11-amino-acid synthetic peptide (P1) significantly inhibited both IgG binding (89.10% ؎ 4.45%) and IgE binding (77.32% ؎ 3.38%) of the standardized diagnostic antigen (SDA) (a well-defined pool of diagnostically relevant allergens and antigens of A. fumigatus). With a panel of sera of ABPA patients, allergic patients with skin test negativity to A. fumigatus, and healthy individuals, P1 showed a higher diagnostic efficiency than SDA (specific IgG, 100%; specific IgE, 98.3%). The diagnostic efficiency of P1 could be attributed to the presence of homologous epitopes in various immunodominant allergens or antigens of A. fumigatus. The ability of P1 to induce histamine release from sensitized mast cells and a Th2 type of cytokine profile in peripheral blood mononuclear cells of ABPA patients suggests its potential for use in intradermal testing. P1 could be further explored for development of a standardized, specific, and sensitive immunodiagnostic test for aspergillosis.

show abstract

Section: Discussionsupporting

confidence: 45%

Section: Discussionmentioning

confidence: 94%

Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen ofAspergillus fumigatus, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis

Madan

Priyadarsiny

Vaid

et al. 2004

Clin Vaccine Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

“…We note the presence of a low homology orthologues of Asp f 1 in the A. terreus genome – an organism previously reported to have no cross reactivity to Asp f 1 [ 29 ]. Hence we examined the predicted structure and epitopes of Asp f 1 and its orthologues to determine whether Asp f 1 epitope orthologues were conserved and whether the orthologues from A. terreus would be predicted to contain credible epitope orthologues using our approach [ 44 , 45 ].…”

Section: Resultsmentioning

confidence: 99%

Comparative genomics of fungal allergens and epitopes shows widespread distribution of closely related allergen and epitope orthologues

2006

View full text Add to dashboard Cite

Background: Allergy is a common debilitating and occasionally life threatening condition. The fungal kingdom contains a number of species that produce a wide range of well defined protein allergens although the vast majority of fungal species have unknown allergenic potential. The recent genome sequencing of a variety of fungi provides the opportunity to assess the occurrence of allergen orthologues across the fungal kingdom. Here we use comparative genomics to survey the occurrence of allergen orthologues in fungi.

show abstract

“…A. fumigatus produces a protein toxin Asp f1 with multiple functions. It is an important allergen, antigen, has ribonuclease activity and is highly cytotoxic [31,32]. A. fumigatus also produces Asp-hemolysin which induces hemolysis of erythrocytes [33].…”

Section: Bioactive Molecules Of Aspergillus Speciesmentioning

confidence: 99%

Aflatoxins: Implications on Health

et al. 2017

View full text Add to dashboard Cite

Environmental occurrence of and other fungal spores are hazardous to humans and animals. They cause a broad spectrum of clinical complications. Contamination of aflatoxins in agri-food and feed due to and result in toxicity in humans and animals. Recent advances in aspergillus genomics and aflatoxin management practices are encouraging to tackle the challenges posed by important aspergillus species.

show abstract

Expression of an epitopic region of AspfI, an allergen/antigen/cytotoxin of Aspergillus fumigatus

Cited by 9 publications

References 19 publications

Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen ofAspergillus fumigatus, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis

Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen ofAspergillus fumigatus, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis

Comparative genomics of fungal allergens and epitopes shows widespread distribution of closely related allergen and epitope orthologues

Aflatoxins: Implications on Health

Contact Info

Product

Resources

About