Expression of an α‐1,3‐glucanase during mycoparasitic interaction of Trichoderma asperellum

Abstract

Trichoderma species have been investigated as biological control agents for over 70 years owing to their ability to antagonize plant pathogenic fungi. Mycoparasitism, one of the main mechanisms involved in the antagonistic activity of Trichoderma strains, depends on the secretion of complex mixtures of hydrolytic enzymes able to degrade the host cell wall. The antifungal activity of an α‐1,3‐glucanase (EC 3.2.1.59, enzymes able to degrade α‐1,3‐glucans and also named mutanases) has been described in T. harzianum and its role in mycoparasitic processes has been suggested. In this study, we report on the purification, characterization and cloning of an exo‐α‐1,3‐glucanase, namely AGN13.2, from the antagonistic fungus T. asperellum T32. Expression at the transcription level in confrontation assays against the strawberry pathogen Botrytis cinerea strongly supports the role of AGN13.2 during the antagonistic action of T. asperellum.

A screening system for carbon sources enhancing β-N-acetylglucosaminidase formation in Hypocrea atroviridis (Trichoderma atroviride)

“…Polysaccharides with the same type of glycosidic linkage are constituents of the cell wall of the majority ascomycetes (Latgé et al, 2005;Schoffelmeer et al, 1999;Tomazett et al, 2005), including plant pathogens (Wolski et al, 2005). Moreover, the formation of a 1,3-a-glucanase has been shown to be part of the mycoparasitic response of H. lixii (Sanz et al, 2005). The stimulation of NAGase activity by a-glucans and D-galactose containing oligosaccharides may thus be part of a mechanism by which H. atroviridis senses the presence of a host cell wall containing chitin.…”

Cloning and Expression of an α-1,3-Glucanase Gene fromBacillus circulansKA-304: The Enzyme Participates in Protoplast Formation ofSchizophyllum commune

“…28,29) -1,3-Glucanases of T. harzianum and T. asperellum (glycosyl hydrolase family 71), 28,29) which secrete when the organisms grow on the fungal cell-wall as a carbon source, have been investigated in detail, and the enzymes showed lytic and antifungal activity against fungal plant pathogens. The enzyme of Trichoderma strains is also a modular enzyme, composed of an N-terminal catalytic domain, a proline-serine-threoninerich linker domain, and a C-terminal -1,3-glucan binding domain (carbohydrate-binding module family 24).…”

“…Trichoderma asperellum, 29) Penicillium purpurogenum, 27) Aspergillus nidulans, 31) and Schizosaccharomyces pombe 32) have been cloned, but the bacterial gene was scarcely cloned, except for that of Bacillus sp. RM1 -1,3-glucanase.…”

“…The presence of mutanases has been also reported in several bacteria [14][15][16][17][18][19][20][21][22] and filamentous fungi. [23][24][25][26][27][28][29][30][31][32][33] Among these -1,3-glucanases, the genes of y To whom correspondence should be addressed. Tel: +81-77-566-1111 ex.…”

Pengaruh Kandang Individu (Single Cages) Dan Kelompok (Group Cages) Terhadap Bobot Akhir, Bobot Karkas, Lemak Abdominal Dan Efisiensi Pakan Pada Ayam Arab Jantan

“…Hal ini sesuai dengan pendapat Widyanata (2013) yang menyatakan bahwa ayam jantan lebih efisien dalam mengubah makanan menjadi daging, sehingga jumlah makanan yang ditimbun sebagai lemak abdominal lebih sedikit. Lemak abdominal yang rendah merupakan hal yang menguntungkan bagi produsen dan konsumen, karena memperbaiki kualitas karkas dengan daging yang rendah (Sanz et al, 2005).…”