Expression of Anchorin CII (Cartilage Annexin V) in Human Young, Normal Adult, and Osteoarthritic Cartilage

Mollenhauer, Jürgen; Mok, Meng Tuck; King, Karen B.; Gupta, Malini; Chubinskaya, Susan; Koepp, Holger; Cole, Ada A.

doi:10.1177/002215549904700209

Cited by 45 publications

(41 citation statements)

References 51 publications

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“…Interestingly, annexin V is also expressed in human osteoarthritic cartilage, but not in healthy human articular cartilage (9,10). Furthermore, type X collagen expression was also detected in human osteoarthritic cartilage, suggesting hypertrophic and terminal differentiation events occur in articular cartilage during osteoarthritis (9).…”

Section: Discussionmentioning

confidence: 98%

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Collagen/Annexin V Interactions Regulate Chondrocyte Mineralization

Kim

Kirsch

2008

Journal of Biological Chemistry

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Physiological mineralization in growth plate cartilage is highly regulated and restricted to terminally differentiated chondrocytes. Because mineralization occurs in the extracellular matrix, we asked whether major extracellular matrix components (collagens) of growth plate cartilage are directly involved in regulating the mineralization process. Our findings show that types II and X collagen interacted with cell surface-expressed annexin V. ] i , alkaline phosphatase activity, and mineralization. In conclusion, the interactions between collagen and annexin V regulate mineralization of growth plate cartilage. Because annexin V is up-regulated during pathological mineralization events of articular cartilage, it is possible that these interactions also regulate pathological mineralization.The extracellular matrix not only plays a major role in maintaining the function of a tissue but it also interacts and communicates directly with the cell via cell receptor/matrix interactions. These interactions play crucial roles in cell adhesion, migration, proliferation, and differentiation. Various types of collagens are the main organic components of extracellular matrices of many tissues. For example, in bone the main organic extracellular matrix component is type I collagen, whereas the main collagenous component in growth plate cartilage is type II collagen (1). Type II collagen is highly expressed in the proliferative and prehypertrophic zones of growth plate cartilage. Just before mineralization starts, type II collagen synthesis is reduced and the hypertrophic growth plate chondrocytes produce type X collagen (2, 3).Several cell surface receptors that mediate cell/matrix interactions have been identified in growth plate chondrocytes. Among these receptors are integrin receptors and non-integrin receptors, like annexin V and CD44. Several integrins have been identified to be expressed by growth plate chondrocytes. Among these integrins are ␣1, ␣2, ␣5, ␣6, ␣V, ␤1, and ␤5. Most of these integrins are expressed in all growth plate zones, whereas some integrins are restricted to certain zones. For example, ␤5 integrin is restricted to the hypertrophic zone (1, 4). The main collagen receptors in growth plate cartilage are ␣1␤1 and ␣2␤1 integrins (5). Annexin V was identified as another protein binding to types II and X collagen, and it has been demonstrated that chondrocytes attach to type II collagen using annexin V (6). It was originally isolated from the chondrocyte membrane fractions as a type II collagen-binding protein (7,8). We and others have shown that annexin V, among other annexins, is highly expressed by hypertrophic and terminally differentiated growth plate chondrocytes (9, 10). Annexins are normally located in the cytoplasm or at the inner plasma membrane surface. However, several annexins have been detected extracellularly or surface-exposed. In addition, several annexins have been shown to act as receptors for a variety of molecules, including extracellular matrix proteins, fetuin A, vitamin D, and other ce...

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Section: Discussionmentioning

confidence: 98%

“…It was originally isolated from the chondrocyte membrane fractions as a type II collagen-binding protein (7,8). We and others have shown that annexin V, among other annexins, is highly expressed by hypertrophic and terminally differentiated growth plate chondrocytes (9,10). Annexins are normally located in the cytoplasm or at the inner plasma membrane surface.…”

mentioning

confidence: 99%

Collagen/Annexin V Interactions Regulate Chondrocyte Mineralization

Kim

Kirsch

2008

Journal of Biological Chemistry

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“…Since the collagen component of normal adult cartilage is believed to have a half-life that exceeds the life of most individuals, the collagen may provide protection to the chondrocyte from apoptotic stimuli. The type II collagen is bound to the chondrocyte's outer cell membrane by attachment to anchorin CII (annexin V; Mollenhauer et al, 1999). This extracellular localization of annexin V is an additional unique feature of the non-apoptotic chondrocyte; in other cell types the presence of annexin V on the outer cell membrane is used as evidence of apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Tumor necrosis factor-? induced DNA cleavage in human articular chondrocytes may involve multiple endonucleolytic activities during apoptosis

Fischer

Mundle

Cole

2000

Microsc. Res. Tech.

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“…Protein synthesis Annexin A5 (NM_001154) [Mollenhauer et al, 1999] CBP1/HSP47 (NM_004353) [Miyashi et al, 1992;Takahashi et al, 1997] Exportin-t (NM_007235)…”

Section: Posttranslational Enzymesmentioning

confidence: 99%

New Chondrocyte Genes Discovered by Representational Difference Analysis of Chondroinduced Human Fibroblasts

Yates¹,

Forbes²,

Glowacki³

2004

Cells Tissues Organs

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This report includes a review of the potential for gene expression analyses to provide new information for solving problems in skeletal repair and regeneration. It focuses on two approaches: high-throughput gene array methods and representational difference analysis (RDA). The principles underlying these methods are presented with experimental tutorials and some applications. Second, this report includes a review of results from applying both approaches to an in vitro model of postnatal chondroinduction by demineralized bone powder (DBP). Human dermal fibroblasts (hDFs) cultured with DBP acquire a chondroblast phenotype and express cartilage-specific matrix proteins after 7 days. We used cDNA macroarrays and RDA to identify the genes that were altered prior to expression of the chondroblast phenotype, i.e., after only 3 days’ culture with DBP. Using a strategy of data management and reduction based upon biological functions, we reported several functional families of genes (cytoskeletal elements, protein synthesis/trafficking, and matrix molecules and their modifiers) that are upregulated during chondroinduction of hDFs. Together with histological and biochemical evidence of the chondroblast phenotype, the gene expression patterns indicate that there are specific stages of induced chondrocyte differentiation in this experimental system. Third, this report includes a new study, in which DBP-regulated genes were used as a data base to derive new information on the cell biology of chondrocytes. The objective was to determine whether a set of genes expressed during induction of chondrocyte differentiation is also expressed by mature articular chondrocytes. Our search of the literature for 59 of the DBP-regulated genes disclosed that expression of 20 of them (33%) had been documented in mature cartilage or chondrocytes. Of the 39 genes not previously documented in cartilage, 11 were tested by RT-PCR and all were found to be expressed in freshly isolated adult human chondrocytes. This review and these new data show how the strategy of high-throughput methods and functional data reduction can expand our knowledge of chondrocyte cell biology.

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Expression of Anchorin CII (Cartilage Annexin V) in Human Young, Normal Adult, and Osteoarthritic Cartilage

Cited by 45 publications

References 51 publications

Collagen/Annexin V Interactions Regulate Chondrocyte Mineralization

Collagen/Annexin V Interactions Regulate Chondrocyte Mineralization

Tumor necrosis factor-? induced DNA cleavage in human articular chondrocytes may involve multiple endonucleolytic activities during apoptosis

New Chondrocyte Genes Discovered by Representational Difference Analysis of Chondroinduced Human Fibroblasts

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