Expression of antimicrobial peptides by bovine endothelial cells

Alva-Murillo, Nayeli; Téllez-Pérez, Ana Dolores; Sagrero-Cisneros, Eduardo; López‐Meza, Joel E.; Ochoa‐Zarzosa, Alejandra

doi:10.1016/j.cellimm.2012.11.016

Cited by 13 publications

(9 citation statements)

References 20 publications

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“…It is well known that LPS enhanced TNF-α expression [2, 12]. To validate our RMMVECs, we first detecetd the TNF-α mRNA expression in RMMVECs in response to LPS stimulation.…”

Section: Resultsmentioning

confidence: 99%

“…So far, the effect of dandelion extracts on endothelial cells is missing. Bovine endothelial cells released more antimicrobial peptides, including mlingual antimicrobial peptide (LAP) and bovine β-defensin 4 (BNBD4) in response to LPS stimulation and those peptides expression were dramatically elevated once with additional treatment with antibody recognizing TNF-α [2]. Therefore, endothelium may play important role during infection.…”

Section: Discussionmentioning

confidence: 99%

“…Additional, the expression of TNF-α, together other inflammatory factors such as interleukin 1 (IL-1), was also elevated even 3-4 h after post-challenge of LPS [3, 34]. Since activation of TNF-α and its corresponding downstream signaling pathways is mainly employed during initiation and development of mastitis [2, 12], new therapy with natural products targeting NTF-α activation is very promising.…”

Section: Introductionmentioning

confidence: 99%

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Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs

Wang

Hong

et al. 2017

BMC Complement Altern Med

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BackgroundMastitis gives rise to big financial burden to farm industry (mainly dairy production) and public health. Its incidence is currently high and therefore, highly effective treatments for therapy, especially with natural products are required. Taraxacum officinale has been reported to use for anti-inflammation. However, its effect on endothelium during mastitis has not been reported.MethodsWe firstly established inflammation experimental model of rat mammary microvascular endothelial cells (RMMVECs). We evaluated the effects of dandelion leaf aqueous extracts (DAE) on LPS-induced production of inflammatory mediators in RMMVECs by enzyme-linked immunosorbent assay and Western blot. We treated RMMVECs with 1 μg/ml LPS for 4 h and then incubated with 10, 100 and 200 μg/mL DAE for 4, 8, 12 and 24 h. The expression (mRNA and protein level) of targets (tumor necrosis factor-alpha (TNF- α) and Intracellular Adhesion Molecule 1 (ICAM1) was analyzed by employing real-time PCR and Western blots. The in vivo anti-inflammatory effect of DAE on mastitis within an Staphylococcus aureus-induced mouse model was also determined.ResultsThe obtained results showed that dandelion extracts at the concentration of 100 and 200 μg/mL could significantly inhibit both TNF-α and ICAM-1 expression in all time points checked while 10 μg/mL of dandelion only suppress both expression at 8 and 12 h post-treatment. The in vivo tests showed that the DAE inhibited the expression of TNF-α and ICAM-1 in a time-dependent manner.ConclusionsAll results suggest that the endothelium may use as as a possible target of dandelion for anti-inflammation.

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“…It is well known that LPS enhanced TNF-α expression [2, 12]. To validate our RMMVECs, we first detecetd the TNF-α mRNA expression in RMMVECs in response to LPS stimulation.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs

Wang

Hong

et al. 2017

BMC Complement Altern Med

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“…HDPs and cytokine genes are generally considered to be synchronously expressed in innate immune response to diverse pathogenic microorganism stimuli. Staphylococcus aureus or lipopolysaccharide (LPS) induces the expression of several HDPs including bovine β-defensin 1 and bovine neutrophil β-defensin 4, after infection (23). In our studies, PBA, which is an odorless derivative of butyric acid naturally produced by colonic bacteria fermentation, increased the endogenous HDP gene expression of pEP2C, pBD-1, and pBD-3 and the cytokine IL-8 and IL-18 production in IPEC J2 cells.…”

Section: Discussionmentioning

confidence: 99%

TLR2/4-mediated NF-κB pathway combined with the histone modification regulates β-defensins and interleukins expression by sodium phenyl butyrate in porcine intestinal epithelial cells

Dou

Han²,

Ma³

et al. 2018

Food & Nutrition Research

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BackgroundHost defense peptides (HDPs) possess direct antibacterial, antineoplastic, and immunomodulatory abilities, playing a vital role in innate immunity. Dietary-regulated HDP holds immense potential as a novel pathway for preventing infection.ObjectiveIn this study, we examined the regulation mechanism of HDPs (pEP2C, pBD-1, and pBD-3) and cytokines (IL-8 and IL-18) expression by sodium phenylbutyrate (PBA).DesignThe effects of PBA on HDP induction and the mechanism involved were studied in porcine intestinal epithelial cell lines (IPEC J2).ResultsIn this study, the results showed that HDPs (pEP2C, pBD-1, and pBD-3) and cytokines (IL-8 and IL-18) expression was increased significantly upon stimulation with PBA in IPEC J2 cells. Furthermore, toll-like receptor 2 (TLR2) and TLR4 were required for the PBA-mediated upregulation of the HDPs. This process occurred and further activated the NF-κB pathway via the phosphorylation of p65 and an IκB α synthesis delay. Meanwhile, histone deacetylase (HDAC) inhibition and an increased phosphorylation of histone H3 on serine S10 also occurred in PBA-induced HDP expression independently with TLR2 and TLR4. Furthermore, p38-MAPK suppressed PBA-induced pEP2C, pBD-1 pBD-3, IL-8, and IL-18 expression, but ERK1/2 failed to abolish the regulation of pBD-3, IL-8, and IL-18. Moreover, epidermal growth factor receptor (EGFR) is involved in PBA-mediated HDP regulation.ConclusionsWe concluded that PBA induced HDP and cytokine increases but did not cause an excessive pro-inflammatory response, which proceeded through the TLR2 and TLR4-NF-κB pathway and histone modification in IPEC J2 cells.

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“…; Alva‐Murillo et al . ). However, information on BNBD‐1 and BNBD‐2 levels in bovine milk is lacking.…”

Section: Introductionmentioning

confidence: 97%

Concentration characteristics of bovine β‐defensin 1 and 2 in fresh bovine milk and infant formula

Wang

Cao

et al. 2015

Int J of Dairy Tech

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The aim of this study was to determine the concentration of defensins BNBD-1 and BNBD-2 in fresh bovine milk and infant formula, and to compare the defensin concentrations of bovine milk and human milk. Abundant BNBD-1 and BNBD-2 was found in fresh bovine colostrum. However, compared to fresh bovine milk, BNBD-1 and BNBD-2 concentrations in infant formula were very low. The BNBDs concentration ranges were also less than b-defensins in human milk. The BNBD-2 concentration in fresh bovine milk was higher than that of BNBD-1.

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Expression of antimicrobial peptides by bovine endothelial cells

Cited by 13 publications

References 20 publications

Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs

Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs

TLR2/4-mediated NF-κB pathway combined with the histone modification regulates β-defensins and interleukins expression by sodium phenyl butyrate in porcine intestinal epithelial cells

Concentration characteristics of bovine β‐defensin 1 and 2 in fresh bovine milk and infant formula

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