Expression of antioxidant enzymes in response to abscisic acid and high osmoticum in tobacco BY-2 cell cultures

Bueno, Pablo; Piqueras, Abel; Kurepa, Jasmina; Savouré, Arnould; Verbruggen, Nathalie; Montagu, Marc Van; Inzé, Dirk

doi:10.1016/s0168-9452(98)00154-x

Cited by 106 publications

(75 citation statements)

References 30 publications

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“…Mn-SOD expression reportedly responds strongly to chilling and oxidative stress (Lee and Lee, 2000;Li et al, 2009), and ABA induces its expression (Bueno et al, 1998). In the present study, pretreating plants with ABA enhanced chilling tolerance by inducing gene expression, such as that of Mn-SOD and POD, involved in oxidative stress defense mechanisms.…”

Section: Effect Of Aba On Antioxidant Gene Expressions In Pepper Leavsupporting

confidence: 53%

Exogenous abscisic acid increases antioxidant enzymes and related gene expression in pepper (Capsicum annuum) leaves subjected to chilling stress

Guo¹,

Chen²,

Gong³

et al. 2012

Genet. Mol. Res.

114

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ABSTRACT. To elucidate how physiological and biochemical mechanisms of chilling stress are regulated by abscisic acid (ABA) pretreatment, pepper variety (cv. 'P70') seedlings were pretreated with 0.57 mM ABA for 72 h and then subjected to chilling stress at 10°/6°C (day/night). Chilling stress caused severe necrotic lesions on the leaves and increased malondialdehyde and H 2 O 2 levels. Activities of monodehydroascorbate reductase (DHAR), dehydroascorbate reductase, glutathione reductase, guaiacol peroxidase, ascorbate peroxidase, ascorbate, and glutathione increased due to chilling stress during the 72 h, while superoxide dismutase and catalase activities decreased during 24 h, suggesting that chilling stress activates the AsA-GSH cycle under catalase deactivation in pepper leaves. ABA pretreatment induced significant increases in the above-mentioned enzyme activities and progressive decreases in ascorbate and glutathione levels. On the other hand, ABA-pretreated seedlings under chilling stress increased superoxide dismutase and guaiacol peroxidase activities and lowered concentrations of other antioxidants compared with untreated chilling-stressed plants. These seedlings showed concomitant decreases in foliage damage symptoms, and levels of malondialdehyde and H 2 O 2 . Induction of Mn-SOD and POD was observed in chilling-stressed plants treated with ABA. The expression of DHAR1 and DHAR2 was altered by chilling stress, but it was higher in the presence than in the absence of ABA at 24 h. Overall, the results indicate that exogenous application of ABA increases tolerance of plants to chilling-induced oxidative damage, mainly by enhancing superoxide dismutase and guaiacol peroxidase activities and related gene expression.

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Section: Effect Of Aba On Antioxidant Gene Expressions In Pepper Leavsupporting

confidence: 53%

Exogenous abscisic acid increases antioxidant enzymes and related gene expression in pepper (Capsicum annuum) leaves subjected to chilling stress

Guo¹,

Chen²,

Gong³

et al. 2012

Genet. Mol. Res.

114

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“…Increased activity of antioxidant enzymes in callus derived from drought tolerant maiz cultivars is reported (Li and van Stadan 1998). Increased activity of these enzymes under PEG stress is also reported in callus cultures of tobacco (Bueno et al 1998). Antioxidant enzymes play a vital role in conferring tolerance to drought and other abiotic stresses.…”

Section: Activity Of Antioxidant Enzymesmentioning

confidence: 82%

In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.)

Srinath

Ftz

2013

Physiol Mol Biol Plants

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A system for in vitro selection of drought tolerant callus lines in sugarcane was developed. High molecular weight PEG was used as selective agent. Selected callus line grew better than non-selected callus when grown on different concentrations of PEG. The activity of antioxidant enzymes like CAT, POX, APX and SOD were high in selected callus than in non-selected callus. Osmolytes like proline and ascorbic acid were at higher levels in selected callus than in nonselected callus, however at higher concentrations (20-30 %) of PEG, levels of proline and ascorbic acid decreased. The frequency of organogenesis and number of plantlets decreased in selected callus than in non-selected callus. The results can be used for in vitro screening and manipulations of sugarcane for improvement of drought tolerance

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“…To cope with oxidative stress, plants have evolved several enzymatic and non-enzymatic systems. The channels by which H 2 O 2 mediates the induction of stomata closure, is by inducing the expression of antioxidant genes SOD (Sakamoto et al 1995;Guan and Scandalios 1998a;Bueno et al 1998), and CAT (Guan and Scandalios 1998b;Guan et al 2000), and increase the activities of antioxidative enzymes such as SOD, CAT, guaiacol peroxidase (GPX), ascorbate peroxidase (APX) and glutathione reductase (GR) in plant tissues (Prasad et al 1994;Gong et al 1998;Bellaire et al 2000). SOD reduces superoxide radicals (O 2 -) to H 2 O 2 , CAT reduces H 2 O 2 to water and dioxygen, APX reduces H 2 O 2 to water and monodehydroascorbate, thus preventing the formation of the highly reactive hydroxyl radical (OH) that can cause lipid peroxidation, protein denaturation, and DNA mutations.…”

Section: Discussionmentioning

confidence: 99%

Changes in H2O2 content and antioxidant enzyme gene expression during the somatic embryogenesis of Larix leptolepis

Zhang

Han

Yang

et al. 2009

Plant Cell Tiss Organ Cult

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Hydrogen peroxide (H 2 O 2 ) content and transcript levels of genes encoding superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) antioxidant enzymes were investigated during different stages of somatic embryogenesis in Larix leptolepis. H 2 O 2 content was lowest on day 0 when embryogenic callus was incubated on Murashige and Skoog (MS) medium supplemented with polyethylene glycol (PEG). This content began to increase when callus was transferred to the same medium but containing abscisic acid (ABA), and reached a peak at day 3 following incubation. The level of H 2 O 2 dropped from day 7 to day 10, peaked at day 21, then dropped again at day 24 and day 35, but increased when somatic embryos reached maturity at day 45. Transcript levels of SOD, CAT, and APX were lowest when somatic embryos were cultured on callus induction medium without ABA. When calli were transferred onto somatic embryo maturation medium, expression patterns of SOD, CAT, and APX varied, and transcript levels at all stages were higher than those at d 0. SOD expression was highest at day 3. Whereas, CAT expression levels were low during early stages of somatic embryogenesis, but increased at day 21, declined at days 24 and 35, and then began to increase again at day 45. APX gene expression patterns were highest at days 3, 21, and 45. These results suggested that ABA was essential for promoting somatic embryogenesis of L. leptolepis. Moreover, ABA induced production of H 2 O 2 and other active oxygen species (AOS), and mediated CAT, SOD, and APX gene expression in somatic embryogenesis of L. leptolepis.

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Expression of antioxidant enzymes in response to abscisic acid and high osmoticum in tobacco BY-2 cell cultures

Cited by 106 publications

References 30 publications

Exogenous abscisic acid increases antioxidant enzymes and related gene expression in pepper (Capsicum annuum) leaves subjected to chilling stress

Exogenous abscisic acid increases antioxidant enzymes and related gene expression in pepper (Capsicum annuum) leaves subjected to chilling stress

In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.)

Changes in H2O2 content and antioxidant enzyme gene expression during the somatic embryogenesis of Larix leptolepis

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