Expression of Aβ42, τ-Protein, p16, p53 in Buccal Epithelium: Prospects for Use in the Diagnostics of Alzheimer’s Disease and Rate of Aging

Zuev, V A; Dyatlova, Anastasiia; Linkova, N. S.; Kvetnaya, T. V.

doi:10.1007/s10517-019-04416-0

Cited by 6 publications

(4 citation statements)

References 14 publications

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“…p16 INK4 expressions were found to be high in blood T cells of old people( Liu et al, 2009 ), in skin ageing ( Ressler et al, 2006 ) and in buccal cells of elderly people ( Zuev et al, 2019 ). These showed that p16 INK4 is a biomarker of aging in the studied samples or tissues.…”

Section: Discussionmentioning

confidence: 99%

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β2-microglobulin is overexpressed in buccal cells of elderly and correlated with expression of p16 and inflammatory genes

Althubiti

2022

Saudi Journal of Biological Sciences

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Section: Discussionmentioning

confidence: 99%

“…In this study, we used buccal cells to examine the expression of β2M in different age groups. In addition, we correlated the expression of β2M with p16 INK4a a biological biomarker of aging( Liu et al, 2009 , Ressler et al, 2006 , Zuev et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

β2-microglobulin is overexpressed in buccal cells of elderly and correlated with expression of p16 and inflammatory genes

Althubiti

2022

Saudi Journal of Biological Sciences

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“…Neuregulin-1 can significantly reduce CoCl 2induced SH-SY5Y cell death by regulating HIF-1α and p53 [30]. It has been proposed that the detection of the Aβ42 peptide, tau, p16 and p53 protein expression levels is a promising method for diagnosing AD and evaluating the aging rate during the development of this disease [31]. VEGFR-2 plays a key role in angiogenesis and is involved in the development of the central nervous system.…”

Section: Discussionmentioning

confidence: 99%

Uncovering the active compounds and effective mechanisms of the dried mature sarcocarp of Cornus officinalis Sieb. Et Zucc. For the treatment of Alzheimer’s disease through a network pharmacology approach

Zhen

Zhang

et al. 2020

BMC Complement Med Ther

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Background: Shanzhuyu (the dried mature sarcocarp of Cornus officinalis Sieb. et Zucc., DMSCO) is a Chinese herb that can be used for the treatment of Alzheimer's disease (AD), but its mechanism remains unknown. The present study aimed to investigate the active ingredients and effective mechanisms of DMSCO for the treatment of AD based on a network pharmacology approach. Methods: The active components of DMSCO were collected from the TCMSP and ETCM databases and the target proteins of these compounds were predicted using TCMSP, SwissTargetPrediction and the STITCH database. The AD-related target proteins were identified from the OMIM, DisGeNet, GEO and GeneCards databases. The network interaction model of the compound-target-disease was established and was used to obtain the key targets of DMSCO on AD through network topology analysis. Subsequently, gene enrichment in Gene Ontology (GO) and KEGG pathways were conducted using the David 6.8 online tool. Results: A total of 30 DMSCO effective compounds and 209 effective drug targets were obtained. A total of 172 AD-related genes and 37 shared targets of DMSCO and AD were identified. A total of 43 key targets for the treatment of AD were obtained from the topological analysis of the DMSCO-AD target network. These key targets were involved in a variety of biological processes, including amyloid deposition, apoptosis, autophagy, inflammatory response and oxidative stress and pathways, such as the PI3K-AKT, MAPK and TNF pathways. Three key compounds, namely ursolic acid, anethole and β-sitosterol were obtained from the analysis of the key targets.

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“…Oral mucosa cells may share common disease-specific characteristics with neurons since it is derived from ectodermal tissue, which is also the embryonic origin of central nervous system ( Paraskevaidi et al, 2020 ). A few studies have focused on Tau and β-amyloid in oral mucosa cells as biomarkers in neurological disorders such as AD and cognitive impairments ( François et al, 2014 , 2016 ; Leifert et al, 2015 ; Arredondo et al, 2017 ; Kose Ozlece et al, 2018 ; Zuev et al, 2019 ). These results suggested that the oral cavity, especially oral mucosa cells, may be a promising source of pathological α-Syn for PD.…”

Section: Introductionmentioning

confidence: 99%

Oral Mucosa Derived α−Synuclein as a Potential Diagnostic Biomarker for Parkinson′s Disease

Zheng

Cai

et al. 2022

Front. Aging Neurosci.

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BackgroundPathological α-synuclein (α-Syn) is not only exclusive to the central nervous system (CNS) in Parkinson’s disease (PD), but also extended to biofluids and peripheral tissues including oral cavity. Both oral mucosa and nervous system are derived from ectodermal tissue, and potentially share common disease-specific characteristics. Oral mucosal exfoliative cytology is a non-invasive technique, which is an easily acceptable for patients and ordinary people. The purpose of this study was to determine the abnormal accumulation of α-Syn in oral mucosa of PD patients and to learn the diagnostic utility of oral mucosa α-Syn for PD.MethodsThe oral mucosa samples were obtained from 57 patients with PD and 51 age-matched controls by cytological brush. Immunofluorescence analysis was used to investigate the presence and subcellular localization of α-Syn, phosphorylated α-Syn at Ser129 (pS129) and oligomeric α-Syn in the oral mucosa cells of PD patients and controls. Images taken as Z-stacks were analyzed for 3D reconstruction to visualize the α-Syn intracellular localization. Then, the concentrations of α-Syn, pS129, and oligomeric α-Syn in oral mucosa samples were measured using electrochemiluminescence assays.ResultsImmunofluorescence images revealed the increased α-Syn, pS129, and oligomeric α-Syn levels in oral mucosa cells of PD patients than age-matched controls. The intracellular distributions of α-Syn species were determine by Z-stack images with 3D reconstruction, and α-Syn was detected in both the nucleus and cytoplasm. However, pS129 was mainly located in the cytoplasm, and oligomeric α-Syn was highly expressed in the nucleus and perinuclear cytoplasm. The concentrations of three α-Syn species were significantly increased in the oral mucosa cell samples of PD patients than controls (α-Syn, p = 0.001; pS129, p = 0.002; oligomeric α-Syn, p = 0.013). In PD patients, the oral mucosa α-Syn and oligomeric α-Syn levels were significantly correlated with Hoehn-Yahr scales (α-Syn, r = 0.495, p = 0.001; oligomeric α-Syn, r = 0.324, p = 0.03). The area under curve (AUC) of ROC analysis using an integrative model including α-Syn, pS129, and oligomeric α-Syn for PD diagnosis was 0.749, with 66.7% sensitivity and 72.5% specificity.ConclusionThis study for the first time demonstrated increased expressions of α-Syn, pS129, and oligomeric α-Syn in oral mucosa cells from PD patients, which serve as useful and non-invasive PD diagnostic biomarkers.

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Expression of Aβ42, τ-Protein, p16, p53 in Buccal Epithelium: Prospects for Use in the Diagnostics of Alzheimer’s Disease and Rate of Aging

Cited by 6 publications

References 14 publications

β2-microglobulin is overexpressed in buccal cells of elderly and correlated with expression of p16 and inflammatory genes

β2-microglobulin is overexpressed in buccal cells of elderly and correlated with expression of p16 and inflammatory genes

Uncovering the active compounds and effective mechanisms of the dried mature sarcocarp of Cornus officinalis Sieb. Et Zucc. For the treatment of Alzheimer’s disease through a network pharmacology approach

Oral Mucosa Derived α−Synuclein as a Potential Diagnostic Biomarker for Parkinson′s Disease

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