Expression of blood group‐related carbohydrate antigens in normal human pancreatic tissue

Abstract

Expression of blood group-related carbohydrate antigens in normal human pancreatic tissue. APMIS 99: 931-940, 1991. The expression of type 1, 2 and 3 chain carbohydrate structures in 15 normal pancreata was investigated by immunohistochemical methods using well-defined monoclonal antibodies. Surgical biopsies of pancreata were obtained from kidney donors while the organs were still perfused. Type 1 chain structures were abundantly expressed including monosialylated(ms)-and disialylated(ds)-Led antigens, which have previously been associated with cancer. Type 2 chain structures were represented by H and Ley antigens and to a lesser extent by the precursor structure N-acetyllactosamine, whereas Le', dimeric Lex, and ms-Lex were only sporadically observed, in contrast to fetal pancreatic tissue, in which Le" has been found to be abundantly expressed. H chain 3 antigen was found in nearly all specimens, whereas precursor structures Tn, sial-Tn and T antigens were absent. Desialylation unmasked the T antigen in all specimens. Absenceimasking of Tn, T and related antigens is of special interest, since these antigens are associated with tumor development in other tissues, and may be of importance in pancreatic cancer diagnostics in the future.

The structure of N‐linked oligosaccharides of human pancreatic bile‐salt‐dependent lipase

“…Furthermore, the Lewis" antigen has been found in pancreatic cancer tissue [30], but is poorly expressed in the adult pancreatic [32]. This antigen may be regarded as an oncodevelopmental antigen [32].…”

“…Furthermore, the Lewis" antigen has been found in pancreatic cancer tissue [30], but is poorly expressed in the adult pancreatic [32]. This antigen may be regarded as an oncodevelopmental antigen [32]. The studies of Wendorf et al [29], revealed that the elastase-1 glycans possessed blood groups A and B, and the related Lewis" and Lewis determinants located on the Mana(l+3)-linked lactosamme antennae.…”

“…The 0-linked glycosylation of FAP was also defective in cancer cells [37]. Accumulation of the tumoral T antigen was demonstrated in fetuses and in certain cancers [32]. This antigen, which represents a precursor for the 0-linked glycan structures, is absent or hidden in normal pancreatic tissue [32].…”

“…Accumulation of the tumoral T antigen was demonstrated in fetuses and in certain cancers [32]. This antigen, which represents a precursor for the 0-linked glycan structures, is absent or hidden in normal pancreatic tissue [32]. Thus, variations in both the N-glycosylation and 0-glycosylation of BSDL in pancreatic pathologies cannot be occluded.…”

Thomsen-Friedenreich-related carbohydrate antigens in normal adult human tissues: a systematic and comparative study

“…To this end, comprehensive knowledge of the occurrence of TFRA in normal adult human tissues is of the utmost importance. Several groups have examined normal tissues in the context of their studies (Mandel et al 1991;Philipsen et al 1991;Therkildsen et al 1994), but a systematic and comprehensive analysis is still lacking. The study of s-TF has only recently become possible by a combination of lectin staining with conventional histochemistry (Sata et al 1990).…”