Expression of bone associated markers by tooth root lining cells, in situ and in vitro

D'Errico, John A.; MacNeil, R. Lamont; Takata, Takashi; Berry, Janice E.; Strayhorn, C.; Somerman, Martha J.

doi:10.1016/s8756-3282(96)00348-1

Cited by 157 publications

(155 citation statements)

References 61 publications

Supporting

Mentioning

145

Contrasting

Unclassified

Order By: Relevance

“…This seems unlikely, however, since cementoblasts abundantly expressed BSP gene in vivo, whereas fibroblastic PDL cells were negative for BSP, as shown in Fig. 1G, a finding that is consistent with those of previous reports (MacNeil et al, 1994;MacNeil et al, 1996;D'Errico et al, 1997). In addition, the Runx2/Cbfa1/Osf2 gene is expressed in PDL cells but not in cementoblasts (Fig.…”

Section: Discussionsupporting

confidence: 91%

A cell line with characteristics of the periodontal ligament fibroblasts is negatively regulated for mineralization and Runx2/Cbfa1/Osf2 activity, part of which can be overcome by bone morphogenetic protein-2

Saito

Yoshizawa

Takizawa

et al. 2002

Journal of Cell Science

106

118

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 91%

A cell line with characteristics of the periodontal ligament fibroblasts is negatively regulated for mineralization and Runx2/Cbfa1/Osf2 activity, part of which can be overcome by bone morphogenetic protein-2

Saito

Yoshizawa

Takizawa

et al. 2002

Journal of Cell Science

106

118

View full text Add to dashboard Cite

show abstract

“…2C-G). The root domains in the vole molar contained all the histological features of a root and a typical expression of Bsp1 in the cementoblasts (D'Errico et al, 1997). We therefore propose that continuously growing teeth in general solve the problem of lack of anchorage, resulting from the absence of a classic root system, by transforming several small domains into the root fate.…”

Section: Root Analogue In the Vole Molarmentioning

confidence: 84%

Root or crown: a developmental choice orchestrated by the differential regulation of the epithelial stem cell niche in the tooth of two rodent species

2003

View full text Add to dashboard Cite

The rodent incisor grows continuously throughout its lifetime. The epithelial stem cell niche is located at the apical end of the tooth and its progeny gives rise to the ameloblasts that form the hard enamel. Previously, mesenchymal FGF10 was shown to support the niche, in conjunction with epithelial Notch signaling. Here we show that in a different continuously growing tooth type, the molar of the sibling vole, a similar regulatory system is in place. Moreover, the identical expression pattern of Bmp4 compared to Fgf10 suggests that BMP4 could also be involved in the regulation of the epithelial stem cell niche.Notch and FGF10 signaling is mainly absent in the mouse molar, which stops growing and develops roots. The regulation of the epithelial stem cell niche seems to be flexible allowing for the existence of different tooth types, such as continuously growing teeth, and high and low crowned molars.

show abstract

“…The differences in the onset and time course of collagen I expression between the periodontal bone and some primary osteoblast cultures is most likely due to the variability of phe- (Rodan, 1981;McCulloch et al, 1987McCulloch et al, , 1989McCulloch and Heersche, 1988 (Pavlin et al, 1998(Pavlin et al, , 2001a (TakanoYamamoto et al, 1994), BSP (Lekic et al, 1996), and osteonectin (Tung et al, 1985)-are also expressed in cementoblasts (MacNeil et al, 1995;D'Errico et al, 1997), while other markers, such as cementum-derived attachment protein and cementumderived growth factor, are specifically associated with cementoblasts (Arzate et al, 1992;Yonemura et al, 1992Yonemura et al, , 1993Pitaru et al, 1993;Liu et al, 1997;Metzger et al, 1998;Saito and Narayanan, 1999). However, there are several differences between bone and cementum, the most obvious being the lack of vascularity and innervation in cementum.…”

Section: (I) Introductionmentioning

confidence: 99%

Effect of Mechanical Loading On Periodontal Cells

Pavlin

Gluhak-Heinrich²

2001

Critical Reviews in Oral Biology & Medicine

View full text Add to dashboard Cite

Mechanical loading is an important regulatory factor in alveolar bone homeostasis, and plays an essential role in maintaining the structure and mass of the alveolar processes throughout lifetime. A better understanding of the cellular and molecular responses of periodontal cells is a prerequisite for further improvements of therapeutic approaches in orthodontics, periodontal and alveolar bone repair and regeneration, implantology, and post-surgical wound healing. The purpose of this review is to provide an insight into some cell culture and animal models used for studying the effects of mechanical loading on periodontal cells, and into the recent developments and utilization of new in vivo animal models. There has been an increased awareness about the need for improvement and development of in vivo models to supplement the widely used cell culture models, and for biological validation of in vitro results, especially in the light of evidence that developmental models may not always reflect bone homeostasis in an adult organism. Due to the limitations of in vivo models, previous studies on mechanical regulation of alveolar bone osteoblasts and cementoblasts mostly focused on proliferative responses, rather than on the stimulation of cell differentiation. To address this problem, we have recently characterized and implemented a mouse osteoinductive tooth movement model for studying mechanically induced regulation of osteoblast-and cementoblast-associated genes. In this model, a defined and reproducible mechanical osteogenic loading is applied during a time course of up to two weeks. Regulation of gene expression in either wild-type or transgenic animals is assessed by a relative quantitative measurement of the level of target mRNAs directly within the subpopulations of periodontal cells. To date, results demonstrate a defined temporal pattern of cellspecific gene regulation in periodontal osteoblasts mechanically stimulated to differentiate and deposit bone matrix. The responses of osteoblast-associated genes to mechanical loading were 10-to 20-fold greater than the increase in the numbers of these cells, indicating that the induction of differentiation and an increase of cell function are the primary responses to osteogenic loading. The progression of the osteoblast phenotype in the intact mouse periodontium was several-fold faster compared with that in cultured cells, suggesting that the mechanical signal may be targeting osteoblast precursors in the state of readiness to respond to an environmental challenge, without the initial proliferative response. An early response of alkaline phosphatase and bone sialoprotein genes was detected after 24 hrs of treatment, followed by a concomitant stimulation of osteocalcin and collagen I between 24 and 48 hrs, and deposition of osteoid after 72 hrs. Although cementoblasts constitutively express biochemical markers similar to those of osteoblasts, distinct responses of osteocalcin, collagen I, and bone sialoprotein genes to mechanical loading were observed in the ...

show abstract

Expression of bone associated markers by tooth root lining cells, in situ and in vitro

Cited by 157 publications

References 61 publications

A cell line with characteristics of the periodontal ligament fibroblasts is negatively regulated for mineralization and Runx2/Cbfa1/Osf2 activity, part of which can be overcome by bone morphogenetic protein-2

A cell line with characteristics of the periodontal ligament fibroblasts is negatively regulated for mineralization and Runx2/Cbfa1/Osf2 activity, part of which can be overcome by bone morphogenetic protein-2

Root or crown: a developmental choice orchestrated by the differential regulation of the epithelial stem cell niche in the tooth of two rodent species

Effect of Mechanical Loading On Periodontal Cells

Contact Info

Product

Resources

About