Expression of bone morphogenetic protein 2 and fibroblast growth factor 2 during bone regeneration using different implant materials as an onlay bone graft in rabbit mandibles

Abstract

1 AbstractPurpose: The purpose of this study was to histologically and immunohistochemically evaluate bone regeneration using three different implant materials in rabbit mandibles and also to compare the bone regenerative capability of these materials in an animal model. Study design:Adult male Japanese white rabbits (n=48, 12-16 weeks, 2.5-3.0 kg) were divided into four groups, consisting of twelve animals each. The implant materials were β-tricalcium phosphate (β-TCP), autologous bone derived from the radius, and recombinant human bone morphogenetic protein-2 (rhBMP-2) with polylactic acid/polyglycolic acid copolymer and gelatin sponge (PGS) complex. After incising along the inferior border of mandible, the materials were implanted as onlay grafts and covered by titanium mesh with screws. No material was implanted into the control group.The rabbits were sacrificed at 2, 4, 8, 12 and 24 weeks postoperatively, and formalin-fixed specimens containing titanium mesh were embedded in acrylic resin. The specimens were stained with hematoxylin and eosin. For immunohistochemical analysis, the specimens were treated with BMP-2 (bone morphogenetic protein-2) and FGF-2 (fibroblast growth factor-2) antibodies. Finally, these were evaluated microscopically.2

Statin-induced bone morphogenetic protein (BMP) 2 expression during bone regeneration: an immunohistochemical study

“…We have reported previously 16 that newly formed bone observed at 8 weeks after implantation of doses of 5μg rh-BMP-2. In other studies, it has been proved that lower doses than 5μg rh-BMP-2 could induce new bone 7,22,23 .…”

BMP2/7 heterodimer is a stronger inducer of bone regeneration in peri-implant bone defects model than BMP2 or BMP7 homodimer

“…BMP2 and BMP7 have been proven to be efficacious in stimulating bone formation in animal models [16][17][18] . BMP2 is known to induce osteogenesis and new bone formation 19,20) . BMP7 is a multifunctional member of the BMP family with multiple effects on bone formation and regeneration [21][22][23] .…”

An experimental study of use of absorbable plate in combination with self-setting α-tricalcium phosphate for orthognathic surgery

“…Therefore, an immuno-histochemical study using BMP antibody was very adequate to examine the possibility of bone-induced effects, but there have been no reports on the expression of BMP induced by α-TCP. In our previous study of onlay graft using rabbit mandible, 49 in the immuno-histochemical examination after treatment with BMP-2 antibody, when the autologous group was compared with the control and β-TCP groups, the cell numbers increased significantly , and there was no significant difference between control and β-TCP in terms of cell number for 2, 4 and 8 weeks. This suggested that bone inductive activity of β-TCP could be low.…”