Expression of cathelicidins mRNA in the goat mammary gland and effect of the intramammary infusion of lipopolysaccharide on milk cathelicidin-2 concentration

Zhang, Gong-Wei; Lai, Songjia; Yoshimura, Y.; Isobe, Naoki

doi:10.1016/j.vetmic.2014.01.029

Cited by 39 publications

(50 citation statements)

References 29 publications

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“…After whole blood collection from the immunized rabbit, the GBD‐1 antibody was purified using a HiTrap Protein G High Performance affinity column (GH HealthCare, Uppsala, Sweden) according to the manufacturer's instructions. Concentration of GBD‐1 in milk was measured by competitive EIA, as described previously (Zhang et al., ,b).…”

Section: Methodsmentioning

confidence: 99%

“…Intramammary infection with E. coli can induce S100A7 expression in the teat cistern, and S100A7 has been shown to be secreted into the milk of dairy cows infected with E. coli (Regenhard, Petzl, Zerbe, & Sauerwein, ). Furthermore, Zhang, Lai, Yoshimura, and Isobe () reported that S100A7 is produced in goat MECs and is secreted into the milk.…”

Section: Introductionmentioning

confidence: 99%

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Effect of steroid hormones on the innate immune response induced by Staphylococcus aureus in the goat mammary gland

Kuwahara

Yoshimura

Isobe

2017

Reprod Domestic Animals

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The objective of this study was to compare the dynamics of innate immune components after intramammary infusion of Staphylococcus aureus (SA) under conditions of high oestrogen and high progesterone in goats. In one group ("E-group"), controlled internal drug release (CIDR) devices were inserted intravaginally from days -11 to -4. Prostaglandin F2α was administered immediately after removal of the CIDR device at day -3, and then oestradiol benzoate (E) was injected intramuscularly once a day from days -2 to 3. Heat-inactivated SA was then administered via intramammary infusion to the left udder at day 0, whilst only saline was infused to the right udder as a control. In a second group ("P-group"), CIDR devices were inserted intravaginally from days -3 to 7 and SA was infused at day 0 in the same way as in the E-group. The milk yield and the concentration of innate immune components (somatic cell count (SCC), lactoferrin (LF), S100A7 and goat ß-defensin 1 (GBD-1)) in the milk were measured. Milk yield decreased drastically in both SA and control udders in the E-group, whereas the P-group exhibited increased milk yield in both SA and control udders. SCC increased after SA infusion in both E- and P-groups, although it was higher in the E-group than in the P-group. There was no significant change in LF concentration in the E-group, but a decrease was observed in the P-group. Concentrations of S100A and GBD-1 were significantly increased after SA infusion in the E-group but not in the P-group. These results suggest that E enhances the innate immune response induced by SA in the goat mammary gland. This effect may be due to the reduction in milk yield and upregulation of innate immune components.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of steroid hormones on the innate immune response induced by Staphylococcus aureus in the goat mammary gland

Kuwahara

Yoshimura

Isobe

2017

Reprod Domestic Animals

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“…The concentration of cathelicidin‐2 in milk increased at 4 h after the intramammary infusion of LPS (Zhang et al . ), suggesting that the secretion of cathelicidin into milk was upregulated by the bacterial stimulation.…”

Section: Cathelicidinmentioning

confidence: 99%

“…In goats, the mRNAs of cathelicidin-1, 2, 3, 6 and 7 were expressed in both the teat and deep region of the mammary gland from healthy and mastitic goats (Zhang et al 2014b). When various regions of goat mammary gland were immunostained with cathelicidin-2 antibody, immunoreactive cathelicidin-2 was not localized in the epithelial cells of the teat skin, teat cistern, or mammary alveoli, whereas it was localized in polymorphonuclear cells in the mammary gland and those collected from blood and milk (Fig.…”

Section: Cathelicidinmentioning

confidence: 99%

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Control mechanisms for producing antimicrobial factors in ruminant mammary gland

Isobe

2017

Animal Science Journal

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Mastitis, a symptom of inflammation in mammary tissue by infection with various kinds of bacteria, causes huge economic losses in the milk industry. One of the popular methods for treatment of mastitis is antibiotics, although this prohibits milk shipping and sometimes causes resistant microbes. Therefore, a new strategy to treat mastitis without antibiotics is eagerly required around the world. Antimicrobial factors belong to innate immunity and can start their function extremely early after bacterial stimulation. These factors have antimicrobial activity for a broad spectrum of bacteria. Elucidation of causal mechanisms and functions of antimicrobial factors in the mammary gland is thought to result in suitable methods for prevention and treatment of mastitis. Therefore, this review introduces traits of some antimicrobial factors and the mechanisms for expressing, producing and secreting them in the mammary gland. For antimicrobial factors, lingual antimicrobial peptide (LAP), S100A7, cathelicidin and lactoferrin are controlled in different sites and different time courses, suggesting that antimicrobial factors play different roles for local defense against bacterial infection in the mammary gland. These findings will contribute to the development of prevention and treatment methods for mastitis.

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Local Heat Treatment of Goat Udders Influences Innate Immune Functions in Mammary Glands

Tsugami

Ishiba

Suzuki

et al. 2021

J Mammary Gland Biol Neoplasia

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Expression of cathelicidins mRNA in the goat mammary gland and effect of the intramammary infusion of lipopolysaccharide on milk cathelicidin-2 concentration

Cited by 39 publications

References 29 publications

Effect of steroid hormones on the innate immune response induced by Staphylococcus aureus in the goat mammary gland

Effect of steroid hormones on the innate immune response induced by Staphylococcus aureus in the goat mammary gland

Control mechanisms for producing antimicrobial factors in ruminant mammary gland

Local Heat Treatment of Goat Udders Influences Innate Immune Functions in Mammary Glands

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