Expression of caveolin‐1 in the interfollicular but not the follicle‐associated epithelial cells in the bursa of fabricius of chickens

Bódi, Ildikó; Minkó, Krisztina; Fölker, Orsolya; Benyeda, Zsófia; Felföldi, Balázs; Magyar, Attila; Kiss, Anna L.; Palya, Vilmos; Oláh, Imré

doi:10.1002/jmor.20749

Cited by 5 publications

(4 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The intensity of eGFP expression between individual cells of the FAE was somewhat variable, suggesting dynamic expression of the CSF1R -transgene (Supplementary Figure 1D). The morphology of bursal CSF1R -transgene expressing cells of the FAE was consistent with their identification as antigen sampling bursal FAE cells, having a tapered or branched basal cytoplasm [Figure 2A; (3, 31–34)]. Murine M cells have a basolateral pocket structure, which contains lymphocytes and mononuclear phagocytes (2).…”

Section: Resultssupporting

confidence: 58%

“…Previously bursal FAE and IFE cells, the latter largely consisting of mucin-secreting cells (5, 31, 35), have been distinguished from each other on the basis of lectin binding on tissue sections (11, 36, 37). CSF1R -transgene expression distinguished the bursal FAE and IFE but some FAE cells appeared to lack CSF1R -transgene expression (Supplementary Figure 1D).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Antigen Sampling CSF1R-Expressing Epithelial Cells Are the Functional Equivalents of Mammalian M Cells in the Avian Follicle-Associated Epithelium

et al. 2019

View full text Add to dashboard Cite

The follicle-associated epithelium (FAE) is a specialized structure that samples luminal antigens and transports them into mucosa-associated lymphoid tissues (MALT). In mammals, transcytosis of antigens across the gut epithelium is performed by a subset of FAE cells known as M cells. Here we show that colony-stimulating factor 1 receptor (CSF1R) is expressed by a subset of cells in the avian bursa of Fabricius FAE. Expression was initially detected using a CSF1R-reporter transgene that also label subsets of bursal macrophages. Immunohistochemical detection using a specific monoclonal antibody confirmed abundant expression of CSF1R on the basolateral membrane of FAE cells. CSF1R-transgene expressing bursal FAE cells were enriched for expression of markers previously reported as putative M cell markers, including annexin A10 and CD44. They were further distinguished from a population of CSF1R-transgene negative epithelial cells within FAE by high apical F-actin expression and differential staining with the lectins jacalin, PHA-L and SNA. Bursal FAE cells that express the CSF1R-reporter transgene were responsible for the bulk of FAE transcytosis of labeled microparticles in the size range 0.02–0.1 μm. Unlike mammalian M cells, they did not readily take up larger bacterial sized microparticles (0.5 μm). Their role in uptake of bacteria was tested using Salmonella, which can enter via M cells in mammals. Labeled Salmonella enterica serovar Typhimurium entered bursal tissue via the FAE. Entry was partially dependent upon Type III secretion system-1. However, the majority of invading bacteria were localized to CSF1R-negative FAE cells and in resident phagocytes that express the phosphatidylserine receptor TIM4. CSF1R-expressing FAE cells in infected follicles showed evidence of cell death and shedding into the bursal lumen. In mammals, CSF1R expression in the gut is restricted to macrophages which only indirectly control M cell differentiation. The novel expression of CSF1R in birds suggests that these functional equivalents to mammalian M cells may have different ontological origins and their development and function are likely to be regulated by different growth factors.

show abstract

Section: Resultssupporting

confidence: 58%

Section: Resultsmentioning

confidence: 99%

Antigen Sampling CSF1R-Expressing Epithelial Cells Are the Functional Equivalents of Mammalian M Cells in the Avian Follicle-Associated Epithelium

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The number of Cav-1-positive IFE cells greatly increased during the first week of life ( Fig. 3), indicating that the IFE cells participate in cholesterol homeostasis in the early life of chickens (Bódi et al, 2018).…”

Section: Resultsmentioning

confidence: 94%

“…Caveolin-1 (Cav-1), an integral membrane protein, is associated with cholesterol in 1 to 1 ratio. Recently it has been published that Cav-1 and cholesterol are expressed in the interfollicular epithelial cells and in the CM capillary web (Bódi et al, 2018).…”

mentioning

confidence: 99%

Coronavirus infection retards the development of the cortico-medullary capillary network in the bursa of Fabricius of chicken

Farsang

Bódi

Fölker

et al. 2018

Acta Veterinaria Hungarica

Self Cite

View full text Add to dashboard Cite

Coronavirus infection delays the development of the cortico-medullary (CM) capillary network which results in retarded development of bursal follicles. The smaller size of the medulla in the coronavirus-infected birds may lead to a lower number of B lymphocytes and bursal secretory dendritic cells, which negatively affects the reactivity and efficacy of the immune system. Contrary to the wild-type infectious bronchitis virus (IBV) strain, infection induced by H120 vaccine virus exerts only a moderate influence on caveolin-1 expression of the CM capillary web and on follicular development compared to the untreated controls.

show abstract

Structure of the avian lymphoid system

2022

View full text Add to dashboard Cite

Expression of caveolin‐1 in the interfollicular but not the follicle‐associated epithelial cells in the bursa of fabricius of chickens

Cited by 5 publications

References 58 publications

Antigen Sampling CSF1R-Expressing Epithelial Cells Are the Functional Equivalents of Mammalian M Cells in the Avian Follicle-Associated Epithelium

Antigen Sampling CSF1R-Expressing Epithelial Cells Are the Functional Equivalents of Mammalian M Cells in the Avian Follicle-Associated Epithelium

Coronavirus infection retards the development of the cortico-medullary capillary network in the bursa of Fabricius of chicken

Structure of the avian lymphoid system

Contact Info

Product

Resources

About