Expression of Cd34 and Myf5 Defines the Majority of Quiescent Adult Skeletal Muscle Satellite Cells

Beauchamp, Jonathan R.; Heslop, Louise A.; Yu, David S.; Tajbakhsh, Shahragim; Kelly, Robert G.; Wernig, A.; Buckingham, Margaret; Partridge, Terence A.; Zammit, Peter S.

doi:10.1083/jcb.151.6.1221

Cited by 805 publications

(794 citation statements)

References 74 publications

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“…Four MRFs (myf5, mrf4, MyoD, myogenin) constitute this family. Except for myf5 (Beauchamp et al, 2000) undifferentiated satellite cells do not express these factors. Activation of satellite cells for muscle regeneration is marked by expression of MRFs in a precisely coordinated temporal pattern (Yun and Wold, 1996).…”

Section: Introductionmentioning

confidence: 99%

A reducing redox environment promotes C2C12 myogenesis: Implications for regeneration in aged muscle

Hansen

Klass

Harris

et al. 2007

Cell Biology International

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Intracellular redox potential of skeletal muscle becomes progressively more oxidized with aging, negatively impacting regenerative ability. We examined the effects of oxidizing redox potential on terminal differentiation of cultured C2C12 myoblasts. Redox potentials were manipulated by changing the culture O 2 environment, by free radical scavenging, or addition of H 2 O 2. Intracellular reactive oxygen species (ROS) production was higher in 20% environmental O 2 and in this condition, redox potential became progressively oxidized compared to cultures in 6% O 2. Treatment with a ROS trapping agent (phenyl-N-tert-butylnitrone, PBN) caused reducing redox potentials and enhanced C2C12 differentiation, while addition of 25 μM H 2 O 2 to cells in 20% O 2 dramatically slowed differentiation. Under these most oxidative conditions, quantitative PCR showed a significant decrease in myogenic basic helix-loop-helix transcription factor expression compared to cultures treated with phenyl PBN or grown in 6% O 2 . Thus, oxidative intracellular environments impair myoblast differentiation, while reducing environments favor myogenesis.

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Section: Introductionmentioning

confidence: 99%

A reducing redox environment promotes C2C12 myogenesis: Implications for regeneration in aged muscle

Hansen

Klass

Harris

et al. 2007

Cell Biology International

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“…More intriguingly, SCs are highly heterogeneous 18,19 , and recent studies have shown that different levels of Pax7 expression produce two subpopulations of SCs-Pax7 Hi and Pax7 Lo -that have distinct biological features in mice 20 . Pax7 Lo cells are primed more for myogenic differentiation and less for self-renewal and higher metabolic status than Pax7 Hi cells.…”

mentioning

confidence: 99%

MicroRNA-431 accelerates muscle regeneration and ameliorates muscular dystrophy by targeting Pax7 in mice

Zhai

et al. 2015

Nat Commun

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Skeletal muscle stem cells, called satellite cells, are a quiescent heterogeneous population. Their heterogeneity is influenced by Pax7, a well-defined transcriptional regulator of satellite cell functions that defines two subpopulations: Pax7 Hi and Pax7 Lo . However, the mechanisms by which these subpopulations are established and maintained during myogenesis are not completely understood. Here we show that miR-431, which is predominantly expressed in the skeletal muscle, mediates satellite cell heterogeneity by fine-tuning Pax7 levels during muscle development and regeneration. In miR-431 transgenic mice, the Pax7 Lo subpopulation is enriched, enhances myogenic differentiation and accelerates muscle regeneration. Notably, miR-431 attenuates the muscular dystrophic phenotype in mdx mice and may be a potential therapeutic target in muscular diseases. miR-431 transgenic mice are a unique genetic model for investigating the cellular features and biological functions of Pax7 Lo satellite cells during muscle development and regeneration.

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“…Myf5 is a transcription factor that contains a basic helix-loop-helix DNA binding domain and belongs to the muscle regulatory factors family. In adult mice, it is considered a typical satellite marker, expressed by quiescent satellite cells and is upregulated during muscle regeneration [26,27]. This makes it an excellent marker of myogenic specification.…”

Section: Bm Cells Enriched For Hsc Surface Markers Undergo Myogenic Smentioning

confidence: 99%

Bone Marrow-Derived Hematopoietic Cells Undergo Myogenic Differentiation Following a Pax-7 Independent Pathway

et al. 2010

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Several reports showed that hematopoietic stem cells (HSCs) participate in muscle regeneration, raising hope for their therapeutic potential for degenerative muscle diseases. However, proof that HSCs are able to reprogram their fate and enter a myogenic pathway, remains elusive. We demonstrate that murine bone marrow (BM)-derived hematopoietic cells, carrying reporter genes controlled by muscle-specific regulatory elements from the Myf5, myosin light chain (MLC3F), or MCK genes, are induced by myoblasts to activate muscle-specific genes. This potential resides in the more undifferentiated progenitors, expressing surface markers typical of HSCs.Comparative gene expression profiling of CD45 1 /Sca1 1 cells isolated from muscle or BM shows that hematopoietic cells participate to muscle regeneration, by undergoing a profound although incomplete myogenic reprogramming on interaction with the muscle microenviroment. These cells undergo specification and differentiation independently from Pax7 and MyoD, and lack Pax7-associated properties, such as self-renewal and proliferation, distinguishing from satellite cells. Our findings indicate that hematopoietic cells, on seeding in the muscle, become a distinct cell population endowed with myogenic potential. STEM CELLS 2010;28:965-973 Disclosure of potential conflicts of interest is found at the end of this article.

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Expression of Cd34 and Myf5 Defines the Majority of Quiescent Adult Skeletal Muscle Satellite Cells

Cited by 805 publications

References 74 publications

A reducing redox environment promotes C2C12 myogenesis: Implications for regeneration in aged muscle

A reducing redox environment promotes C2C12 myogenesis: Implications for regeneration in aged muscle

MicroRNA-431 accelerates muscle regeneration and ameliorates muscular dystrophy by targeting Pax7 in mice

Bone Marrow-Derived Hematopoietic Cells Undergo Myogenic Differentiation Following a Pax-7 Independent Pathway

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