Expression of cell adhesion molecules during human preimplantation embryo development

Bloor, Debra; Metcalfe, Anthony D.; Rutherford, Anthony J.; Brison, Daniel R.; Kimber, Susan J.

doi:10.1093/molehr/8.3.237

Cited by 101 publications

(94 citation statements)

References 59 publications

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“…CAR protein has already been shown to contribute to the formation of tight junction and to have a barrier function in epithelial cells by forming a complex with ZO1 and recruiting it to homotypic cell-cell contacts (Cohen et al 2001). Our co-stainings of CAR with ZO1 and OCLN confirm the connection of membrane CAR with tight junctions in human preimplantation embryos and add to previous findings on the timing and the character of cellular adhesion organisation during human preimplantation development (Gualtieri et al 1992, Hardy et al 1996, Bloor et al 2002. In fact, the proper formation of the intercellular connections is critical for normal embryogenesis (Hardy et al 1996, Alikani 2005.…”

Section: Discussionsupporting

confidence: 83%

“…In parallel to the first differentiation in the human embryo (TE vs ICM), outer cells at compaction stage and later TE cells in the expanding blastocyst differ from the inner cells by the prominent accumulation of CAR on the membrane. Further investigation at the expanded blastocyst stage indicates the localisation of CAR protein at the tight junctions of the outer TE cells, as well as the inner cells (both PE and EPI) similar to ZO1 and OCLN (Bloor et al 2002, Alikani 2005.…”

Section: Discussionmentioning

confidence: 95%

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CAR expression in human embryos and hESC illustrates its role in pluripotency and tight junctions

Krivega¹,

Geens²,

Velde³

2014

Reproduction

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Coxsackie virus and adenovirus receptor, CXADR (CAR), is present during embryogenesis and is involved in tissue regeneration, cancer and intercellular adhesion. We investigated the expression of CAR in human preimplantation embryos and embryonic stem cells (hESC) to identify its role in early embryogenesis and differentiation. CAR protein was ubiquitously present during preimplantation development. It was localised in the nucleus of uncommitted cells, from the cleavage stage up to the precursor epiblast, and corresponded with the presence of soluble CXADR3/7 splice variant. CAR was displayed on the membrane, involving in the formation of tight junction at compaction and blastocyst stages in both outer and inner cells, and CAR corresponded with the full-length CAR-containing transmembrane domain. In trophectodermal cells of hatched blastocysts, CAR was reduced in the membrane and concentrated in the nucleus, which correlated with the switch in RNA expression to the CXADR4/7 and CXADR2/7 splice variants. The cells in the outer layer of hESC colonies contained CAR on the membrane and all the cells of the colony had CAR in the nucleus, corresponding with the transmembrane CXADR and CXADR4/7. Upon differentiation of hESC into cells representing the three germ layers and trophoblast lineage, the expression of CXADR was downregulated. We concluded that CXADR is differentially expressed during human preimplantation development. We described various CAR expressions: i) soluble CXADR marking undifferentiated blastomeres; ii) transmembrane CAR related with epithelial-like cell types, such as the trophectoderm (TE) and the outer layer of hESC colonies; and iii) soluble CAR present in TE nuclei after hatching. The functions of these distinct forms remain to be elucidated.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 95%

CAR expression in human embryos and hESC illustrates its role in pluripotency and tight junctions

Krivega¹,

Geens²,

Velde³

2014

Reproduction

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“…In the tetrahedral group, the number of contact points varied from four to six. Compaction normally occurs during the fourth cleavage division [34] and is mediated through E-cadherin [35,36]. Cleavage abnormalities or loss of physical contact between blastomeres at an early The table shows the quality of the transferred and non-transferred embryos on day 5…”

Section: Discussionmentioning

confidence: 99%

Developmental capacity and pregnancy rate of tetrahedral- versus non-tetrahedral-shaped 4-cell stage human embryos

Cauffman

Verheyen

Tournaye

2014

J Assist Reprod Genet

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Purpose The arrangement of the blastomeres within the 4-cell stage embryo reflects the orientation of the cleavage planes during the second division. To examine their relevance, the developmental capacity and the pregnancy rate were compared between tetrahedral-shaped and non-tetrahedralshaped 4-cell stage human embryos. Methods The study included 3,546 4-cell stage embryos. The arrangement of the blastomeres at the 4-cell stage was annotated as being tetrahedral or non-tetrahedral on day 2 of preimplantation development. Embryo quality was compared on day 3 and day 5. Pregnancy rates were calculated per single embryo transfer on day 3 or day 5. Results In total, 2,803 4-cell stage embryos (79 %) displayed a tetrahedral arrangement and 743 (21 %) displayed a non-tetrahedral arrangement. Tetrahedralshaped embryos developed more into high-quality embryos on day 3 (p<0.001) and day 5 (p=0.036) and had a higher blastulation rate (p=0.009). Though, the number of high-quality embryos selected for transfer did not differ between both groups on day 3 (p=0.167) and day 5 (p~1). Three hundred thirty single embryo transfers were analysed. No significant difference in clinical pregnancy was found between both groups after transfer on day 3 (p=0.209) and day 5 (p=0.653). Conclusions The arrangement of the blastomeres according to their previous cleavage planes was correlated to the developmental potential of the 4-cell stage embryo up to the blastocyst stage. If embryo transfers are performed on day 3 and day 5 of development using embryos of adequate quality, the blastomere arrangement at the 4-cell stage had no predictable value regarding pregnancy success.

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“…a5b1, avb3, avb5, and avb6 are expressed by the trophectoderm during early apposition and adhesion (Sutherland et al, 1993;Bloor et al, 2002), while a1b1, a6b1, and a7b1 tend to dominate during embryonic invasion (Bowen and Hunt, 2000). In early blastocysts, a5b1 is initially expressed by cells located between the inner cell mass and the blastocoele cavity.…”

Section: Endometrial Integrinsmentioning

confidence: 99%

Molecular mechanisms of membrane interaction at implantation

Davidson

Coward

2016

Birth Defects Research Pt C

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Successful pregnancy is dependent upon the implantation of a competent embryo into a receptive endometrium. Despite major advancement in our understanding of reproductive medicine over the last few decades, implantation failure still occurs in both normal pregnancies and those created artificially by assisted reproductive technology (ART). Consequently, there is significant interest in elucidating the etiology of implantation failure. The complex multistep process of implantation begins when the developing embryo first makes contact with the plasma membrane of epithelial cells within the uterine environment. However, although this biological interaction marks the beginning of a fundamental developmental process, our knowledge of the intricate physiological and molecular processes involved remains sparse. In this synopsis, we aim to provide an overview of our current understanding of the morphological changes which occur to the plasma membrane of the uterine endothelium, and the molecular mechanisms that control communication between the early embryo and the endometrium during implantation. A multitude of molecular factors have been implicated in this complex process, including endometrial integrins, extracellular matrix molecules, adhesion molecules, growth factors, and ion channels. We also explore the development of in vitro models for embryo implantation to help researchers investigate mechanisms which may underlie implantation failure. Understanding the precise molecular pathways associated with implantation failure could help us to generate new prognostic/diagnostic biomarkers, and may identify novel therapeutic targets.

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Expression of cell adhesion molecules during human preimplantation embryo development

Cited by 101 publications

References 59 publications

CAR expression in human embryos and hESC illustrates its role in pluripotency and tight junctions

CAR expression in human embryos and hESC illustrates its role in pluripotency and tight junctions

Developmental capacity and pregnancy rate of tetrahedral- versus non-tetrahedral-shaped 4-cell stage human embryos

Molecular mechanisms of membrane interaction at implantation

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