Expression of chemosensory proteins in hairs on wings of Locusta migratoria (Orthoptera: Acrididae)

Abstract

The hairs on the wings of Locusta migratoria were observed and mapped using light microscopy, as well as by scanning and transmission electron microscopy. Based on their ultrastructure, we can distinguish four main types of hairs on the wings of adult L. migratoria, viz, short, medium and long hairs, and sensilla chaetica. The long hairs are located only on the ventral surface of the hindwing, whereas the other three types are present both on the dorsal and ventral surfaces of forewing and hindwing in both sexes. Medium hairs and sensilla chaetica are significantly more abundant on the dorsal surface of forewings in both females and males, than on the ventral surface, whereas the opposite was observed for short hairs (P < 0.01). No significant difference between males and females was observed in the density of any type of hairs (P > 0.1). Several dendritic branches, enveloped by a dendrite sheath, are situated in the lymph cavity of sensilla chaetica. Instead, no dendritic structure was observed in the cavity of the other three types of hairs. Immunocytochemical localization of chemosensory proteins (CSPs) was performed on ultrathin sections of hairs on wings. The antiserum against chemosensory proteins from L. migratoria (LmigCSP‐II) strongly labelled sensilla chaetica, with gold granules only found in the outer sensillum lymph. In addition, the epidermal cell membrane of the wing was stained by the antiserum against LmigCSP‐II. The other three types of hairs were never labelled. The results indicate that the wings might involve in contact chemoreception process.

Identification and expression pattern of candidate olfactory genes in Chrysoperla sinica by antennal transcriptome analysis

“…Our study showed that most of OBPs had relatively high expressed in antennae compared with other tissues, consistent with the antennae being the main olfactory organ in C. sinica and suggesting an olfactory role for these genes. OBP5 is rather broadly expression with relatively high levels of expression in antennae and wings of both female and male; therefore it may also be involved in gustatory function, as wings play somewhat gustatory roles in insects (Zhou et al, 2008). OBP8 was expressed in antennae, head, and legs at relatively high levels.…”

BdorCSP2 Is Important for Antifeed and Oviposition-Deterring Activities Induced by Rhodojaponin-III against Bactrocera dorsalis

“…High expression of BdorCSP2 in adult implied more olfactory receptor neurons (ORNs) in the adult and the essential roles of sense organs for adults to enable the flies to attract a spouse for courtship or to find the proper hosts for oviposition after mating (Figure 3) [44]. Tissue distribution showed BdorCSP2 was highly expressed in legs, which suggested this CSP might involve in feeding, oviposition activities and other contacting chemoreception processes via legs [12]. Our result was consistent with previous study that CSPs were expressed in some putative contact chemosensory sensilla in Schistocerca gregaria .…”

“…The insects could detect external stimuli through hair-like sensilla on the chemosensory organs, such as antenna, maxillary and tarsus [12]. More specifically, insects use olfaction system to search and orientate for locations of potential host plants [13], and then after landing, contacting chemoreception could provide the primary basis for host plant selection and discrimination [14].…”

The chemosensilla on tarsi of Locusta migratoria (Orthoptera: Acrididae): Distribution, ultrastructure, expression of chemosensory proteins

“…Members of the CSP family were reported in Lepidoptera (Maleszka and Stange, 1997;Picimbon et al, 2001), Orthoptera (Angeli et al, 1999;Picimbon et al, 2000;Ban et al, 2003;Jin et al, 2005), Hymenoptera (Danty et al, 1998;Forêt and Maleszka, 2006;Forêt et al, 2007), Blattoidea (Picimbon and Leal, 1999), and Phasmatodea Mameli et al, 1996;Marchese et al, 2000). Odorant-binding proteins and some chemosensory proteins seem to be involved in chemoreception based on their binding of semiochemicals (Campanacci et al, 2001(Campanacci et al, , 2003Ban et al, 2003;Mosbah et al, 2003) and their specific localization in the lymph of chemosensilla of antennae as well as legs and wings (Angeli et al, 1999;Jin et al, 2005;Zhou et al, 2008).…”

“…Many chemosensory sensilla have also been found on the maxillary and labial palps as well as wings of locusts. These chemosensilla may play a major role in food selection (Blaney andChapman, 1969, 1970;Klein, 1981;Jin et al, 2006;Zhou et al, 2008). In contrast to our detailed knowledge of the structure and physiology of chemosensilla on antennae, wings and palps, little is known about those present on tarsi of locusts.…”