BdorCSP2 Is Important for Antifeed and Oviposition-Deterring Activities Induced by Rhodojaponin-III against Bactrocera dorsalis

Yi, Xin; Zhao, Haiming; Dong, Xiaolin; Wang, Peidan; Hu, Meiying; Zhong, Guohua

doi:10.1371/journal.pone.0077295

Cited by 27 publications

(29 citation statements)

References 52 publications

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“…All BdorCSPs were very similar to the CSP family of other Diptera species. The unigene Bdorcsp1 was predicted to have the same function as its paralog, Bdorcsp2 , in feeding and oviposition [ 18 ]. The identified CSPs from B .…”

Section: Resultsmentioning

confidence: 99%

“…The odorant-binding proteins (OBPs), chemosensory proteins (CSPs), and sensory neuron membrane proteins (SNMPs) are involved in the perireceptor events, and the odorant receptors (ORs) and ionotropic receptors (IRs) play an important role in the signal transduction process [ 14 ]. OBPs that originate from the auxiliary cells and that are secreted into the aqueous sensillum lymph of the antenna (and/or maxillary palps) are thought to be the first proteins that bind with the semiochemicals to facilitate the transport of hydrophobic odorants through the aqueous surroundings to olfactory receptors [ 15 , 16 ]; some CSPs also function in this process [ 17 , 18 ]. After being released from the OBPs or CSPs, the semiochemicals are then transferred to the receptors on the olfactory sensory neuron dendritic membranes, ORs and/or IRs.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Male- and Female-Specific Olfaction Genes in Antennae of the Oriental Fruit Fly (Bactrocera dorsalis)

et al. 2016

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The oriental fruit fly (Bactrocera dorsalis) is a species of tephritid fruit fly, endemic to Southeast Asia but also introduced to many regions of the US, and it is one of the major pest species with a broad host range of cultivated and wild fruits. Although males of B. dorsalis respond strongly to methyl eugenol and this is used for monitoring and estimating populations, the molecular mechanism of the oriental fruit fly olfaction has not been elucidated yet. Therefore, in this project, using next generation sequencing technologies, we sequenced the transcriptome of the antennae of male and female adults of B. dorsalis. We identified a total of 20 candidate odorant binding proteins (OBPs), 5 candidate chemosensory proteins (CSPs), 35 candidate odorant receptors (ORs), 12 candidate ionotropic receptors (IRs) and 4 candidate sensory neuron membrane proteins (SNMPs). The sex-specific expression of these genes was determined and a subset of 9 OR genes was further characterized by qPCR with male and female antenna, head, thorax, abdomen, leg and wing samples. In the male antennae, 595 genes showed a higher expression, while 128 genes demonstrated a higher expression in the female antennae. Interestingly, 2 ORs (BdorOR13 and BdorOR14) were highly and specifically expressed in the antennae of males, and 4 ORs (BdorOR13, BdorOR16, BdorOR18 and BdorOR35) clustered with DmOR677, suggesting pheromone reception. We believe this study with these antennae-enriched OBPs, CSPs, ORs, IRs and SNMPs can play an important role in the detection of pheromones and general odorants, and so in turn our data improve our current understanding of insect olfaction at the molecular level and provide important information for disrupting the behavior of the oriental fruit fly using chemical communication methods.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of Male- and Female-Specific Olfaction Genes in Antennae of the Oriental Fruit Fly (Bactrocera dorsalis)

et al. 2016

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“…Due to the direct damage to crops and negative effects on export markets, they are considered a quarantine pest. Our group has conducted a series of studies concerning the chemosensory system of oriental fruit fly (Yi et al, 2013 , 2014 ). In B. dorsalis , chemosensory perception plays a key role in behavior regulation such as, host-seeking, mating and oviposition.…”

Section: Introductionmentioning

confidence: 99%

Olfactory Plasticity: Variation in the Expression of Chemosensory Receptors in Bactrocera dorsalis in Different Physiological States

Jin

Zhou

et al. 2017

Front. Physiol.

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Changes in physiological conditions could influence the perception of external odors, which is important for the reproduction and survival of insect. With the alteration of physiological conditions, such as, age, feeding state, circadian rhythm, and mating status, insect can modulate their olfactory systems accordingly. Ionotropic, gustatory, and odorant receptors (IR, GR, and ORs) are important elements of the insect chemosensory system, which enable insects to detect various external stimuli. In this study, we investigated the changes in these receptors at the mRNA level in Bactrocera dorsalis in different physiological states. We performed transcriptome analysis to identify chemosensory receptors: 21 IRs, 12 GRs, and 43 ORs were identified from B. dorsalis antennae, including almost all previously known chemoreceptors in B. dorsalis and a few more. Quantitative real-time polymerase chain reaction analysis revealed the effects of feeding state, mating status and time of day on the expression of IR, GR, and OR genes. The results showed that expression of chemosensory receptors changed in response to different physiological states, and these changes were completely different for different types of receptors and between male and female flies. Our study suggests that the expressions of chemosensory receptors change to adapt to different physiological states, which may indicate the significant role of these receptors in such physiological processes.

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“…Adult females choose their oviposition sites through olfactory receptors or gustatory sensilla organs that detect volatile and nonvolatile signals, respectively. The mechanism involves multigene families of chemoreceptors [45,46]. Santos et al [18,19] reported evidence that the ovipositionstimulant action of WSMoL involves gustatory stimuli.…”

Section: Resultsmentioning

confidence: 99%

Biotechnological value of Moringa oleifera seed cake as source of insecticidal lectin against Aedes aegypti

Oliveira

Silva

Lima

et al. 2016

Process Biochemistry

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BdorCSP2 Is Important for Antifeed and Oviposition-Deterring Activities Induced by Rhodojaponin-III against Bactrocera dorsalis

Cited by 27 publications

References 52 publications

Identification of Male- and Female-Specific Olfaction Genes in Antennae of the Oriental Fruit Fly (Bactrocera dorsalis)

Identification of Male- and Female-Specific Olfaction Genes in Antennae of the Oriental Fruit Fly (Bactrocera dorsalis)

Olfactory Plasticity: Variation in the Expression of Chemosensory Receptors in Bactrocera dorsalis in Different Physiological States

Biotechnological value of Moringa oleifera seed cake as source of insecticidal lectin against Aedes aegypti

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