Expression of CK19 is an independent predictor of negative outcome for patients with squamous cell carcinoma of the tongue

Ernst, Jutta; Ikenberg, Kristian; Apel, Barbara; Schumann, Desiree M.; Huber, Gerhard; Studer, Gabriela; Rordorf, Tamara; Rössle, Matthias; Korol, Dimitri; Bredell, Marius

doi:10.18632/oncotarget.12691

Cited by 14 publications

(10 citation statements)

References 17 publications

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“…However, in head and neck cancer, new cut-off levels of 131 and 300 copies/μl were proposed by different studies [9, 36]. Head and neck tumours are squamous cell carcinomas and expression of KRT19 is a poor prognostic marker [37]. Most breast, gastric, colorectal, and between the 40 and 60% of lung malignancies, are adenocarcinomas.…”

Section: Discussionmentioning

confidence: 99%

Detection of lymph node metastasis in lung cancer patients using a one-step nucleic acid amplification assay: a single-centre prospective study

et al. 2019

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Background The use of one-step nucleic acid amplification (OSNA) allows for lymph node (LN) metastasis to be detected rapidly and accurately. We conducted a prospective single-centre clinical trial to evaluate OSNA assay in detecting LN metastasis of lung cancer. Patients and methods A total of 705 LNs from 160 patients with clinical stage IA to IVA lung cancer were included in this study. The LNs were divided and submitted to routine histological diagnosis and OSNA assay and the results were compared. We also examined keratin 19 expression of different histological types lung primary tumours. Results When the cut-off value was set to 250 copies/µl, the concordance rate between the two methods was 96.17% and the sensitivity 97.14%. Discordant results were observed in 27 LNs of 21 patients. Most of these discordant results were molecular micrometastasis expressing a very low number of copies with negative histology. Most thoracic tumours were positive for keratin 19. Conclusions Our data show that the OSNA assay might be a useful and sensitive method to diagnose LN metastasis in lung cancer and could be applied to intraoperative decision-making in personalised lung cancer surgery based on LN status and a more accurate staging of patients.

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Section: Discussionmentioning

confidence: 99%

Detection of lymph node metastasis in lung cancer patients using a one-step nucleic acid amplification assay: a single-centre prospective study

et al. 2019

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“…CK19 is mainly expressed in the basal cells of the normal oral squamous epithelium, but it is upregulated and abnormally distributed in OSCC. Therefore, CK19 overexpression is an early event in oral mucosa carcinogenesis and its abnormal expression is used as one of the reliable biomarkers in the early diagnosis of OSCC 49‐51 . Furthermore, some studies reported that the loss of cytokeratin 13 expression and cytokeratin 17 overexpression may be useful in distinguishing oral precancerous lesions from reactive oral lesions with atypical epithelium, suggesting that both CK13 and CK17 may play an important role in the progression of OSCC 52‐54 (Figure 1).…”

Section: Alteration Of the Cytoskeletonmentioning

confidence: 99%

Epithelial‐to‐mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities

Ling

Cheng

Tao

2020

Intl Journal of Cancer

147

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Oral squamous cell carcinoma (OSCC) is the most common malignancy representing 90% of all forms of oral cancer worldwide. Although great efforts have been made in the past decades, the 5‐year survival rate of OSCC patients is no more than 60% due to tumor metastasis and subsequent recurrence. The metastasis from the primary site is due to a complex process known as epithelial‐to‐mesenchymal transition (EMT). During the EMT, epithelial cells gradually acquire the structural and functional characteristics of mesenchymal cells, leading to the upregulation of cell migration and the promotion of tumor cell dissemination. Therefore, EMT attracted broad attention due to its close relationship with cancer invasion and metastasis. Therefore, in the present review, an extensive description of the current research on OSCC and the role of EMT in this cancer type is provided, including diverse EMT markers, regulatory networks and crucial EMT‐inducing transcription factors in OSCC. Moreover, a brief summary was made regarding the current application of EMT‐correlated indexes in the prognostic analysis of OSCC patients, and the potential therapeutic approaches against OSCC and difficulties in the development of an effective anti‐EMT treatment are discussed. Our aim is to provide novel insights to develop new strategies to combat OSCC by targeting EMT.

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“…Aside from being employed as a diagnostic biomarker, CKs are also found to harbour potential prognostic value in various epithelial cell-associated carcinomas [ 48 – 50 ]. Importantly, numerous studies have shown the significant correlation between CKs (especially 8, 18 and 19) and poor prognosis of HNSCC [ 50 , 51 ], warranting further investigation. In summary, we have reported significantly elevated expression levels of CK 8 and 18 in saliva collected from HPV-negative patients, with simultaneously elevated expression levels of CK 17, 18 and 19 in saliva collected from HPV-positive HNSCC patients.…”

Section: Discussionmentioning

confidence: 99%

The overexpression of salivary cytokeratins as potential diagnostic biomarkers in head and neck squamous cell carcinomas

et al. 2017

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BackgroundCytokeratin (CK) intermediate filaments are demonstrated to have enormous potential in regulating cellular motility and cancer progression. There are more than 20 divergent CKs that have been identified, of which CK 8, 17, 18 and 19 are reported to be elevated in the tumour biopsies of head and neck cancer squamous cell carcinoma (HNSCC) patients. However, CK expression profiles in the saliva of HNSCC patients have not been investigated. We aim to investigate the mRNA expression profiles of CKs in saliva collected from healthy controls, HPV-negative and -positive HNSCC patients.MethodsOral rinse samples were collected from 42 cancer-free healthy controls (age-matched) and patients who have been diagnosed with HPV-negative (n = 20) and -positive (n = 48) HNSCC.ResultsHere, we report that the mRNA expression profiles of CKs differed in saliva collected from healthy controls and HNSCC patients. The mRNA expression levels of CK 8 and 18 were significantly elevated in saliva collected from HPV-negative HNSCC patients; whilst, CK 17 and 19 were expressed at a higher mRNA level in saliva collected from HPV-positive HNSCC patients compared to healthy controls. Importantly, receiver operating characteristic (ROC) analysis showed salivary CK 8 and 18 to have superior sensitivity and specificity in discriminating the HPV-negative HNSCC patients from healthy controls (80% and 86%) as well as between HPV-negative and -positive HNSCC patients (75% and 81%).ConclusionIn summary, we have demonstrated that an aberrant expression of salivary CKs may serve as a potential non-invasive diagnostic biomarker in HNSCC.

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Expression of CK19 is an independent predictor of negative outcome for patients with squamous cell carcinoma of the tongue

Cited by 14 publications

References 17 publications

Detection of lymph node metastasis in lung cancer patients using a one-step nucleic acid amplification assay: a single-centre prospective study

Detection of lymph node metastasis in lung cancer patients using a one-step nucleic acid amplification assay: a single-centre prospective study

Epithelial‐to‐mesenchymal transition in oral squamous cell carcinoma: Challenges and opportunities

The overexpression of salivary cytokeratins as potential diagnostic biomarkers in head and neck squamous cell carcinomas

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