Expression of class I and class II MHC antigens in neuromuscular diseases

McDouall, Rhoda; Dünn, Michael J.; Dubowitz, Victor

doi:10.1016/0022-510x(89)90023-3

Cited by 100 publications

(69 citation statements)

References 23 publications

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“…It is known that the MHC class I antigen, which inhibits the action of natural killer cells (Smythe et al, 2001), is down-regulated with the formation of mature myofibers in normal muscle (Ponder et al, 1983;Karpati, 1990;Pavlath et al, 1994). Even though regenerating myofibers (such as those observed within dystrophic muscle) display some increase in MHC-I expression (Emslie- Smith et al, 1989;McDouall et al, 1989), the fusion of donor cells could serve to protect them from host immune responses, but consequently limit the degree of dystrophin restoration. An ability to proliferate prior to fusion would enhance the degree of restoration, however, it could also lead to exposure to cytotoxic host cell responses, unless the proliferation was rapid enough.…”

Section: Discussionmentioning

confidence: 99%

The role of CD34 expression and cellular fusion in the regeneration capacity of myogenic progenitor cells

Jankowski

Deasy

Cao

et al. 2002

Journal of Cell Science

103

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Characterization of myogenic subpopulations has traditionally been performed independently of their functional performance following transplantation. Using the preplate technique, which separates cells based on their variable adhesion characteristics, we investigated the use of cell surface proteins to potentially identify progenitors with enhanced regeneration capabilities. Based on previous studies, we used cell sorting to investigate stem cell antigen-1 (Sca-1) and CD34 expression on myogenic populations with late adhesion characteristics. We compared the regeneration efficiency of these sorted progenitors, as well as those displaying early adhesion characteristics, by quantifying their ability to regenerate skeletal muscle and restore dystrophin following transplantation into allogenic dystrophic host muscle. Identification and utilization of late adhering populations based on CD34 expression led to differential regeneration, with CD34-positive populations exhibiting significant improvements in dystrophin restoration compared with both their CD34-negative counterparts and early adhering cell populations. Regenerative capacity was found to correspond to the level of myogenic commitment, defined by myogenic regulatory factor expression, and the rate and degree of induced cell differentiation and fusion. These results demonstrate the ability to separate definable subpopulations of myogenic progenitors based on CD34 expression and reveal the potential implications of defining myogenic cell behavioral and phenotypic characteristics in relation to their regenerative capacity in vivo.

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Section: Discussionmentioning

confidence: 99%

The role of CD34 expression and cellular fusion in the regeneration capacity of myogenic progenitor cells

Jankowski

Deasy

Cao

et al. 2002

Journal of Cell Science

103

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“…In contrast to normal muscle tissue, myocytes of PM patients express HLA class I molecules at their surface (37)(38)(39), allowing the presentation of muscular autoantigens to cytotoxic CD8 ϩ T cells. Furthermore, it was recently reported that forced expression of MHC class I molecules in skeletal muscle of young mice induces a PM-like disease accompanied by mononuclear cell muscle infiltrates (40).…”

Section: Discussionmentioning

confidence: 99%

Severe Perturbations of the Blood T Cell Repertoire in Polymyositis, But Not Dermatomyositis Patients

Benveniste

Chérìn

Maisonobe

et al. 2001

The Journal of Immunology

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Polymyositis and dermatomyositis are diseases characterized by muscle weakness and muscle inflammatory infiltrates. Their pathogenesis remains unclear. A central role for endomysial autoaggressive CD8+ T cells is suspected in polymyositis and for perivascular B cells in dermatomyositis. We compared the T cell repertoire of 10 polymyositis and 10 dermatomyositis patients by immunoscope, a method providing a global assessment of the T cell repertoire and a sensitive detection of clonal T cell expansions. Samples were analyzed qualitatively and quantitatively in the blood (unsorted cells and CD4+ and CD8+ cells) and in muscle infiltrates. Dramatic perturbations of the T cell repertoire were observed in the blood of polymyositis but not dermatomyositis patients (p < 0.0005), the latter being undistinguishable from controls. These perturbations were due to oligoclonal expansions of CD8+ T cells and most blood clonal expansions were also found in muscle. These results indicate that the pathogenesis of polymyositis and dermatomyositis is different and reinforce the view that polymyositis but not dermatomyositis is an autoimmune CD8+ T cell-mediated disease. Moreover, this method may be helpful for the differential diagnosis of polymyositis and dermatomyositis and for noninvasive follow-up of polymyositis patients.

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“…Unlike the inflammatory infiltrates, MHC-I expression is still detectable even after short-term immunosuppressive treatment and in patients with chronic myositis [9,15]. Moreover, MHC-I staining often occurs early, preceding the inflammatory infiltrates, and is present diffusely throughout the biopsy and is thus less likely to be affected by sampling error [16]. Nevertheless, even though it has been considered helpful in distinguishing IIM from other muscle diseases, it is not specific and also occurs in other myopathies [17].…”

Section: Mhc-i Is Expressed But Is Undetectable Immunohistochemicallymentioning

confidence: 99%

An analysis of the sensitivity and specificity of MHC-I and MHC-II immunohistochemical staining in muscle biopsies for the diagnosis of inflammatory myopathies

Cruz

Luo

Miller

et al. 2014

Neuromuscular Disorders

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MURDOCH RESEARCH REPOSITORYThis is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Abstract 1 Abstract 3 AbstractAlthough there have been several previous reports of immunohistochemical staining for MHC antigens in muscle biopsies, there appears to be a lack of consensus about its routine use in the diagnostic evaluation of biopsies from patients with suspected inflammatory myopathy. Positive MHC-I staining is nonspecific but is widely used as a marker for inflammatory myopathy, while the role of MHC-II staining is not clearly defined. We investigated the sensitivity and specificity of MHC-I and II immunostaining for the diagnosis of inflammatory myopathy in a large group of biopsies from a single reference laboratory. Positive staining for MHC-I was found to have a high sensitivity in biopsies from patients with inflammatory myopathy but a very low specificity, as it was also common in other non-inflammatory myopathies and neurogenic disorders.On the other hand, MHC-II positivity had a much higher specificity in all major subgroups of inflammatory myopathy, especially inclusion body myositis. The findings indicate that the combination of MHC-I and MHC-II staining results in a higher degree of specificity for the diagnosis of inflammatory myopathy and that in biopsies with inflammation, positive MHC-II staining strongly supports the diagnosis of an immunemediated myopathy. We recommend that immunohistochemical staining for both MHC-I and MHC-II should be included routinely in the diagnostic evaluation of muscle biopsies from patients with suspected inflammatory myopathy. However, as the sensitivity and interpretation of MHC staining may depend on the technique used, further studies are needed to compare procedures in different centres and develop standardised protocols.Abstract 4

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Expression of class I and class II MHC antigens in neuromuscular diseases

Cited by 100 publications

References 23 publications

The role of CD34 expression and cellular fusion in the regeneration capacity of myogenic progenitor cells

The role of CD34 expression and cellular fusion in the regeneration capacity of myogenic progenitor cells

Severe Perturbations of the Blood T Cell Repertoire in Polymyositis, But Not Dermatomyositis Patients

An analysis of the sensitivity and specificity of MHC-I and MHC-II immunohistochemical staining in muscle biopsies for the diagnosis of inflammatory myopathies

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