Expression of claudin-3 in the esophagus and larynx of rat reflux model

Xu, Xiangde; Hu, Ying; Wang, Yan; Lai, Chui Jin; Zhang, Tian Yu

doi:10.1016/j.anl.2014.05.011

Cited by 2 publications

(2 citation statements)

References 22 publications

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“… 9 , 10 , 14 , 15 Another report showed that claudin-3 could be a sensitive indicator of reflux laryngitis in rats. 16 Considering these reports together with our results, it is plausible that co-administration of rebamipide with PPI profoundly recovers the permeability of esophageal mucosa in rat GERD models.…”

Section: Discussionsupporting

confidence: 69%

Additive Effects of Rebamipide Plus Proton Pump Inhibitors on the Expression of Tight Junction Proteins in a Rat Model of Gastro-Esophageal Reflux Disease

et al. 2018

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Background/AimsThe aim of this study was to investigate the effects of rebamipide on tight junction proteins in the esophageal mucosa in a rat model of gastroesophageal reflux disease (GERD).MethodsGERD was created in rats by tying the proximal stomach. The rats were divided into a control group, a proton pump inhibitor (PPI) group, and a PPI plus rebamipide (PPI+R) group. Pantoprazole (5 mg/kg) was administered intraperitoneally to the PPI and PPI+R groups. An additional dose of rebamipide (100 mg/kg) was administered orally to the PPI+R group. Mucosal erosions, epithelial thickness, and leukocyte infiltration into the esophageal mucosa were measured in isolated esophagi 14 days after the procedure. A Western blot analysis was conducted to measure the expression of claudin-1, -3, and -4.ResultsThe mean surface area of mucosal erosions, epithelial thickness, and leukocyte infiltration were lower in the PPI group and the PPI+R group than in the control group. Western blot analysis revealed that the expression of claudin-3 and -4 was significantly higher in the PPI+R group than in the control group.ConclusionsRebamipide may exert an additive effect in combination with PPI to modify the tight junction proteins of the esophageal mucosa in a rat model of GERD. This treatment might be associated with the relief of GERD symptoms.

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Section: Discussionsupporting

confidence: 69%

Additive Effects of Rebamipide Plus Proton Pump Inhibitors on the Expression of Tight Junction Proteins in a Rat Model of Gastro-Esophageal Reflux Disease

et al. 2018

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“…In healthy vocal fold epithelium, the tight junction complex seals neighboring epithelial cells by connecting adjacent cell membranes together restricting particle movement. In this study, we did not find any significant change in the expression of occludin and claudin3, two transmembrane proteins that occlude adjacent epithelial cells [ 46 – 49 ] and are expressed in larynx [ 10 , 50 ]. This indicates that the barrier function reduction is unlikely to be associated with altered gene expression of tight junction proteins.…”

Section: Discussionmentioning

confidence: 63%

Acute Acrolein Exposure Induces Impairment of Vocal Fold Epithelial Barrier Function

2016

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Acrolein is a ubiquitous pollutant abundant in cigarette smoke, mobile exhaust, and industrial waste. There is limited literature on the effects of acrolein on vocal fold tissue, although there are clinical reports of voice changes after pollutant exposures. Vocal folds are responsible for voice production. The overall objective of this study was to investigate the effects of acrolein exposure on viable, excised vocal fold epithelial tissue and to characterize the mechanism underlying acrolein toxicity. Vocal fold epithelia were studied because they form the outermost layer of the vocal folds and are a primary recipient of inhaled pollutants. Porcine vocal fold epithelia were exposed to 0, 50, 100, 500, 900 or 1300 μM of acrolein for 3 hours; the metabolic activity, epithelial resistance, epithelial permeability, tight junction protein (occludin and claudin 3) expression, cell membrane integrity and lipid peroxidation were investigated. The data demonstrated that acrolein exposure at 500 μM significantly reduced vocal fold epithelial metabolic activity by 27.2% (p≤0.001). Incubation with 100 μM acrolein caused a marked increase in epithelial permeability by 130.5% (p<0.05) and a reduction in transepithelial electrical resistance (TEER) by 180.0% (p<0.001). While the expression of tight junctional protein did not change in acrolein-treated samples, the cell membrane integrity was significantly damaged with a 45.6% increase of lipid peroxidation as compared to controls (p<0.05). Taken together, these data provide evidence that acute acrolein exposure impairs vocal fold epithelial barrier integrity. Lipid peroxidation-induced cell membrane damage may play an important role in reducing the barrier function of the epithelium.

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Expression of claudin-3 in the esophagus and larynx of rat reflux model

Cited by 2 publications

References 22 publications

Additive Effects of Rebamipide Plus Proton Pump Inhibitors on the Expression of Tight Junction Proteins in a Rat Model of Gastro-Esophageal Reflux Disease

Additive Effects of Rebamipide Plus Proton Pump Inhibitors on the Expression of Tight Junction Proteins in a Rat Model of Gastro-Esophageal Reflux Disease

Acute Acrolein Exposure Induces Impairment of Vocal Fold Epithelial Barrier Function

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