Expression of cupA gene cluster responsible for biofilm formation in Pseudomonas aeruginosa is enhanced against subinhibitory concentration of carbapenems

Deshamukhya, Chandrayee; Saikia, Ranjan; Das, Bhaskar Jyoti; Paul, Deepjyoti; Dhar, Debadatta; Bhattacharjee, Amitabha

doi:10.1016/j.genrep.2021.101427

Gene Reports

2022

DOI: 10.1016/j.genrep.2021.101427

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Expression of cupA gene cluster responsible for biofilm formation in Pseudomonas aeruginosa is enhanced against subinhibitory concentration of carbapenems

Chandrayee Deshamukhya

Ranjan Saikia

Bhaskar Jyoti Das

et al.

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Cited by 3 publications

(1 citation statement)

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“…Patients with metabolic diseases, hematological diseases, and malignant tumors as well as those who have undergone surgery or have weakened immunity can be easily infected with this bacterium. It can cause bedsore, abscess, suppurative otitis media, bronchitis, pneumonia, and other diseases [ 4 ]. In addition, the pathogen can infect pigs, poultry, mink, and other animals, suggesting that it is a typical zoonotic pathogen [ 2 , 11 , 19 ].…”

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confidence: 99%

Extracellular polysaccharide of Lactobacillus plantarum enhance immune efficacy of oprH gene recombinant subunit vaccine from Pseudomonas aeruginosa

TIAN,

GONG,

ZHU

et al. 2023

The Journal of Veterinary Medical Science

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To evaluate the immune enhancement effect of the extracellular polysaccharide of Lactobacillus plantarum on oprH recombinant subunit vaccine from Pseudomonas aeruginosa , a recombinant subunit vaccine of oprH (rOprH vaccine) was developed. The EP-rOprH vaccine was prepared with the extracellular polysaccharide of L. plantarum as an adjuvant. Mice were vaccinated with the rOprH and EP-rOprH vaccines, and the outer membrane protein (OMP) and inactivated vaccines were used as controls. The levels of serum antibody, interferon-γ (IFN-γ), interleukin (IL-2), and IL-4 were determined after vaccination. Finally, the protective efficacy of the vaccine was evaluated after challenge with virulent P. aeruginosa . Following vaccination, the serum antibody levels were significantly higher in mice vaccinated with the EP-rOprH vaccine than in those vaccinated with the rOprH vaccine ( P <0.05). Moreover, the serum antibody levels detected in the EP-rOprH vaccine group were similar to those detected in the OMP vaccine group when P. aeruginosa suspension was used as the coating antigen. However, the levels in the EP-rOprH vaccine group were higher than those in the OMP vaccine and inactivated vaccine groups when the purified rOprH protein was used as the coating antigen ( P <0.05). The level of IFN-γ, IL-2, and IL-4 in mice vaccinated with the EP-rOprH vaccine was significantly higher than that in mice vaccinated with the rOprH vaccine ( P <0.05) and comparable to that in mice vaccinated with the OMP vaccine. The protective rates were 65%, 80%, 80%, and 95% with the rOprH, EP-rOprH, OMP, and inactivated vaccines, respectively. Thus, the extracellular polysaccharide of L. plantarum significantly enhanced the immune response and protection provided by the recombinant subunit vaccine of oprH .

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mentioning

confidence: 99%

Extracellular polysaccharide of Lactobacillus plantarum enhance immune efficacy of oprH gene recombinant subunit vaccine from Pseudomonas aeruginosa

TIAN,

GONG,

ZHU

et al. 2023

The Journal of Veterinary Medical Science

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Doxycycline combined manure microbes to enhances biofilm formation of the soil plastisphere and increases the surface bio-risk of microplastics vehicle

Liu

Deng

Niu

et al. 2023

Chemical Engineering Journal

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Orange Peel Lactiplantibacillus plantarum: Development of A Mucoadhesive Nasal Spray with Antimicrobial and Anti-inflammatory Activity

Corazza,

Pizzi,

Parolin

et al. 2024

Pharmaceutics

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Background/Objectives: Due to the high frequency and severity of upper respiratory bacterial infections, probiotics could offer a new medical approach. We explored the antibacterial and anti-inflammatory properties of the new strain Lactiplantibacillus plantarum BIA and formulated a nasal spray. Methods: L. plantarum BIA was isolated from orange peel and taxonomically identified through 16S rRNA gene sequencing. Its antibacterial activity was tested against Pseudomonas aeruginosa, Streptococcus pyogenes, Bacillus subtilis, Escherichia coli, and Staphylococcus aureus, while anti-inflammatory potential was evaluated by Griess assay. BIA genome was fully sequenced and analyzed to assess its safety. BIA was formulated in a freeze-dried matrix, containing prebiotics and cryoprotectants, to be reconstituted with a polymer solution. Solutions containing two types of hydroxypropyl methylcellulose (HPMC) and hyaluronic acid were evaluated as resuspending media and compared in terms of pH, viscosity, and mucoadhesion ability. The biological activity of BIA formulated as nasal spray was verified together with the stability of the selected formulations. Results: L. plantarum BIA inhibited human pathogens’ growth and showed anti-inflammatory activity and a safe profile. In the best-performing formulation, the probiotic is lyophilized in 10% fructooligosaccharides, 0.1% ascorbic acid, and 0.5% lactose and reconstituted with HPMC high viscosity 1% w/v. This composition ensured the probiotic’s viability for up to six months in its dried form and one week after reconstitution. It also allowed interaction with the nasal mucosa, preserving its antimicrobial and anti-inflammatory activities. Conclusion: The developed nasal spray could become a promising formulation in the field of nasal infectious and inflammatory diseases.

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Expression of cupA gene cluster responsible for biofilm formation in Pseudomonas aeruginosa is enhanced against subinhibitory concentration of carbapenems

Cited by 3 publications

References 17 publications

Extracellular polysaccharide of <i>Lactobacillus plantarum</i> enhance immune efficacy of <i>oprH</i> gene recombinant subunit vaccine from <i>Pseudomonas aeruginosa</i>

Extracellular polysaccharide of <i>Lactobacillus plantarum</i> enhance immune efficacy of <i>oprH</i> gene recombinant subunit vaccine from <i>Pseudomonas aeruginosa</i>

Doxycycline combined manure microbes to enhances biofilm formation of the soil plastisphere and increases the surface bio-risk of microplastics vehicle

Orange Peel Lactiplantibacillus plantarum: Development of A Mucoadhesive Nasal Spray with Antimicrobial and Anti-inflammatory Activity

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