Expression of Cyclooxygenase‐1 and ‐2 in the Porcine Endometrium during the Oestrous Cycle and Early Pregnancy

Blitek, Agnieszka; Wacławik, Agnieszka; Kaczmarek, Monika M.; Stadejek, Tomasz; Pejsak, Z.; Ziȩcik, Adam J.

doi:10.1111/j.1439-0531.2006.00646.x

Cited by 50 publications

(38 citation statements)

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“…In gilts, developing conceptuses secrete estrogens to signal its presence in the uterus [34], and the profile of E2 secretion by developing embryos coincides with estrogen receptor expression in the endometrium [32]. Changes occurred in the endometrium as a result of conceptus estrogen secretion include increased PGHS-2 expression [20,21] and PGE2 secretion [35]. The existence of the Hoxa10 / Pghs2 / PGE2 axis has previously been shown in uterine tissues of mice.…”

Section: Discussionmentioning

confidence: 99%

“…The rate-limiting enzyme in PG production is prostaglandin H synthase (PGHS; also known as cyclooxygenase), which catalyzes the conversion of arachidonic acid to PGH2 [19], the common substrate for various PGs. Expression of PGHS-2 in the endometria of cyclic and early pregnant gilts has previously been demonstrated [20,21]. Interestingly, Hoxa10 null mice lack Pghs2 expression in stromal cells of the endometrium.…”

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confidence: 99%

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Effect of Steroids on HOXA10 mRNA and Protein Expression and Prostaglandin Production in the Porcine Endometrium

Blitek

Kiewisz

Wacławik

et al. 2010

Journal of Reproduction and Development

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Abstract. The homeobox A (HOXA) family of genes is responsible for segmental development of the female reproductive tract during embryogenesis. However, HOXA10 has been shown to be essential not only for uterus development, but also for implantation. Persistent expression and steroid-dependent regulation of this gene has been demonstrated in adult human, primate, murine and canine uteri. Moreover, HOXA10-dependent expression of prostaglandin H synthase-2 (PGHS-2), a key enzyme in prostaglandin production, has been previously detected. The role of the HOXA10 gene in the porcine uterus is not well established. Therefore, the present studies were undertaken to 1) examine the effect of E2 and P4 on HOXA10 mRNA and protein content in the endometrium collected on day 9 of the estrous cycle and 2) determine the PGHS-2 protein expression and PGE2 and PGF2α secretion from endometrial tissue in response to steroid treatment. Endometrial explants collected from mature gilts on day 9 of the estrous cycle were incubated with E2 (1-100 nM), P4 (10-1000 nM) or E2 (10 nM) and P4 (100 nM) for 24 h. E2 alone or E2 in the presence of P4 increased HOXA10 mRNA expression in the endometrium (P<0.05). The HOXA10 protein level was upregulated in response to E2, P4 and both steroids administered simultaneously (P<0.05). Moreover, E2 and P4 stimulated PGHS-2 protein expression in cultured endometrial explants. PGE2, but not PGF2α, secretion increased in the presence of E2 (P<0.05). However, the release of both prostaglandins was decreased after treatment of endometrial explants with the highest dose of P4 (P<0.01). These results demonstrate that E2 and P4 are important regulators of HOXA10 gene expression in the adult porcine endometrium during the mid-luteal phase of the estrous cycle. Additionally, the similar profiles of endometrial HOXA10 and PGHS-2 expression in the presence of E2 and P4 indicate that both genes are simultaneously regulated by steroids in the porcine uterus. Key words: Endometrium, Estradiol, HOXA10, PGHS-2, Pig, Progesterone, Prostaglandins (J. Reprod. Dev. 56: [643][644][645][646][647][648] 2010) he uterine endometrium is one of the most dynamic tissues in the organism, undergoing morphological and physiological changes during each estrous cycle. Disorders in endometrial development may lead to lack of or improper implantation, pregnancy failure or infertility. Among the different hormones, estradiol (E2) and progesterone (P4) of ovarian origin are crucial regulators of endometrial receptivity and embryo-maternal cross-talk in many species [1][2][3][4]. It is obvious that the action of P4 in priming the uterus is absolutely essential to attain uterine receptivity for implantation, but the requirement of E2 of ovarian origin is speciesspecific [1]. However, the molecular mechanisms that direct the ordered proliferation, differentiation and cell death associated with the normal menstrual or estrous cycles are still not well characterized. Good candidates for regulation of gene expression in the endometrium are ho...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Effect of Steroids on HOXA10 mRNA and Protein Expression and Prostaglandin Production in the Porcine Endometrium

Blitek

Kiewisz

Wacławik

et al. 2010

Journal of Reproduction and Development

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“…Parent and Fortier (2005) demonstrated that mPGES expression decreased from the beginning of the cycle until days 13-15 and then increased rapidly to reach higher expression at the time of luteolysis (Days 16-18). Waclawik et al (2006) reported that mPGES-1 expression increased on days 13-15 compared to day 18-21 but no significant variation was observed throughout the cycle in pig. Mondal (2009) reported that expression of mPGES-1 mRNA could not be detected in the cyclic endometrium of buffalo during the first (days 3-5) and second (days 6-15) stages but was observed in the endometrium during the third stage (days 16-21) of estrous cycle.…”

Section: Prostaglandin E Synthase (Pges)mentioning

confidence: 99%

“…Currently, the role of COX-3 is not known. However, reports suggest a possible role in pain sensitivity, based on the studies focused on the mechanism of action of acetaminophen (paracetamol), evoked as a selective inhibitor of COX-3 (Blitek et al 2006). Although COX-1 deficient female mice are fertile, they have specific defects in parturition, whereas COX-2 deficient female mice are infertile with abnormalities in ovulation, fertilization, implantation, and decidualization.…”

Section: Cyclooxygenase 2 (Cox 2)mentioning

confidence: 99%

“…(Mondal et al 2006). Expression of the PGES mRNA has been evaluated in a variety of tissues such as uterus, placenta, corpus luteum and ovary (Arosh et al 2004;Parent and Fortier 2005;Waclawik et al 2006;. The expression of PGES mRNA and protein increased with gestation in ovine placenta but no further change with onset or progression of labour or during cortisol infusion (Martin et al 2002).…”

Section: Prostaglandin E Synthase (Pges)mentioning

confidence: 99%

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Genes Regulating Maternal Recognition of Pregnancy in Domestic Animals: an Update

Mor

Mondal

Reddy

et al. 2015

Braz. arch. biol. technol.

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Early embryonic mortality is one of the main sources of reproductive wastages and major constraints for full exploitation of the production potential of livestock. The survivality of embryo during early embryonic life is mostly dependent on the efficiency with which the maternal recognition of pregnancy (MRP) is established. Maternal recognition of pregnancy involves molecular dialogue between the trophoblast of conceptus and uterine endometrium. Embryonic development to the blastocyst stage and uterine differentiation to the receptive environment are crucial for successful establishment of the embryo-uterine cross-talk that leads to the initiation and progression of successful implantation. Unravelling the complex intricate molecular and cellular dialogues betweenthe conceptus and uterine environment will facilitate development of strategies to augment early embryo survivality.

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Rapid conceptus elongation in the pig: An interleukin 1 beta 2 and estrogen‐regulated phenomenon

Geisert

Whyte

Meyer

et al. 2017

Molecular Reproduction Devel

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Establishment and maintenance of pregnancy in the pig involves activating many physiological, cellular, and molecular signaling pathways between the developing conceptus and hormonally regulated maternal endometrium. Rapid elongation of the pig trophoblast allows for the establishment of sufficient placental surface area for the transport of nutrients to the fetus throughout pregnancy. Estrogens secreted by the conceptus during elongation act on uterine epithelia to induce secretion of uterine factors required for conceptus development and for preventing endocrine secretion of prostaglandin F2α, which would cause luteolysis. Thus, trophoblast expansion within the uterine lumen during early gestation is an essential process for implantation and maintenance of pregnancy in species with an epitheliochorial form of placentation. In the pig, rapid conceptus elongation involves the unique expression of interleukin-1 beta 2 (IL1B2), which establishes pro-inflammatory effects that may be tempered by the spatiotemporal secretion of estrogen from the conceptuses. The present review provides current information on pig conceptus remodeling and signaling via estrogen and IL1B2 pathways, as well as endometrial responses to those conceptus factors leading to establishment of pregnancy.embryo, endometrium, placenta, pregnancy[The] unique capacity of pig conceptuses to rapidly remodel and elongate, compared with other mammals, is an essential mechanism developed to establish pregnancy.

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Expression of Cyclooxygenase‐1 and ‐2 in the Porcine Endometrium during the Oestrous Cycle and Early Pregnancy

Cited by 50 publications

References 56 publications

Effect of Steroids on HOXA10 mRNA and Protein Expression and Prostaglandin Production in the Porcine Endometrium

Effect of Steroids on HOXA10 mRNA and Protein Expression and Prostaglandin Production in the Porcine Endometrium

Genes Regulating Maternal Recognition of Pregnancy in Domestic Animals: an Update

Rapid conceptus elongation in the pig: An interleukin 1 beta 2 and estrogen‐regulated phenomenon

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