Expression of Cyclooxygenase-2 (COX-2) in Colorectal Adenocarcinoma: an Immunohistochemical and Histopathological Study

Mahmoud, Abla Sayed; Umair, Ayesha; Azzeghaiby, Saleh Nasser; Alqahtani, Fahad Hussain; Hanouneh, Salah; Tarakji, Bassel

doi:10.7314/apjcp.2014.15.16.6787

Cited by 18 publications

(15 citation statements)

References 20 publications

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“…However, they did not observe any association between clinicopathological parameters and expression of COX-2. The results of the present study are also supported by the observation of Mahmoud et al, (2014), who have found no association between COX-2 expression and the clinicopathological features except for association between COX-2 expression and gender. Contradictory to results of present study, COX-2 expression was significantly linked with the depth of tumor invasion in another study (Lim et al, 2007).…”

Section: Discussionsupporting

confidence: 90%

Over-Expression of Cyclooxygenase-2 in Colorectal Cancer Patients

Negi

Rana

Gupta

et al. 2019

Asian Pac J Cancer Prev

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100,000, respectively. Whereas, AAR for colon cancer in women is 3.9 per 100,000. Colon cancer ranks 8th and rectal cancer ranks 9th among men in India (NCRP, 2013).The development of colorectal cancer is a highly complex and multistep process and is linked to mutations that may occur in the oncogenes K-ras and APC or in the tumor suppressor gene p53, thereby causing uncontrolled proliferation of cells. Cell proliferation is pivotal in tumorigenesis and Cyclooxygenases (COXs) are important regulatory

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Section: Discussionsupporting

confidence: 90%

Over-Expression of Cyclooxygenase-2 in Colorectal Cancer Patients

Negi

Rana

Gupta

et al. 2019

Asian Pac J Cancer Prev

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“…It is well documented that COX2 expressions are higher in CRC than those in the paracarcinoma tissues. [ 10 11 ] In this work, we showed that both mRNA and protein levels of COX2 were upregulated in CRC by bioinformatics and immunohistochemistry analysis. In addition, cytoplasmic and cytomembranous COX2 is associated with pathological stage, tumor recurrence, metastasis, and poor patient prognosis, and is an independent prognostic factor in CRC.…”

Section: Discussionmentioning

confidence: 89%

Correlated non-nuclear COX2 and low HER2 expression confers a good prognosis in colorectal cancer

Zhou

Huang

Jiang

et al. 2018

Saudi J Gastroenterol

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Background/Aims:COX2 and HER2 are shown to be critical in the regulation of cancer progression. However, the prognostic value of nuclear COX2 in colorectal cancer (CRC) and its relationship with HER2 still remains unknown. In this study, the expression and biological significance of COX2 and HER2 were evaluated in CRC at mRNA and protein levels.Materials and Methods:RNA-Seq data of CRC were downloaded from TCGA, and 229 CRC and 50 non-cancerous subjects were enrolled in this study. Bioinformatics and immunohistochemistry analysis was performed based on the obtained data. Survival analysis was conducted to identify factors associated with overall survival of CRC patients.Results:We showed that mRNA and protein levels of COX2 and HER2 were upregulated in CRC compared with the adjacent tissues. COX2 protein levels and nuclear COX2 expression were correlated with a poor prognosis of CRC patients. In addition, we also revealed that nuclear COX2 expression was positively associated with HER2 expression. Non-nuclear COX2 combined with low HER2 expression, was negatively correlated with Duke's stage and lymph node metastasis, predicting the best outcomes for CRC patients. In addition, our data indicated that non-nuclear COX2 combined with low HER2 expression is an independent prognostic factor for CRC after surgical resection.Conclusion:The study suggests that nuclear COX2 in combination with HER2 can serve as potential biomarkers for the clinical diagnosis and prognosis of CRC, and targeted inhibition of COX2 and HER2 might be an alternative strategy for the management of CRC.

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“…With respect to molecular mechanisms of action, mounting data confirm that the inhibition of this process may be a critical event in the development of colonic tumors (3, 24, 29, 30). Cyclooxygenase-2 (COX-2) is an enzyme that converts arachidonic acid (20:4, n−6) to prostaglandin E 2 , and has been reported to be involved in colonic tumor development (31, 32). Studies have shown that prostanglandins produced by COX-2 promote colon cell proliferation and inhibit apoptosis (32).…”

Section: Discussionmentioning

confidence: 99%

In Vivo Regulation of Colonic Cell Proliferation, Differentiation, Apoptosis, and P27Kip1 by Dietary Fish Oil and Butyrate in Rats

Hong

Turner

Murphy

et al. 2015

Cancer Prevention Research

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We have shown that dietary fish oil is protective against experimentally-induced colon cancer and the protective effect is enhanced by co-administration of pectin. However, the underlying mechanism(s) have not been fully elucidated. We hypothesized that fish oil with butyrate, a pectin fermentation product, protects against colon cancer initiation by decreasing cell proliferation and increasing differentiation and apoptosis through a p27Kip1 mediated mechanism. Rats were provided diets of corn or fish oil, with/without butyrate, and terminated 12, 24 or 48 h post azoxymethane (AOM) injection. Proliferation (Ki-67), differentiation (Dolichos Biflorus Agglutinin), apoptosis (TUNEL) and p27Kip1 (cell cycle mediator) were measured in the same cell within crypts in order to examine the coordination of cell cycle as a function of diet. DNA damage (N7-methylguanine) was determined by quantitative immunohistochemical analysis. Dietary fish oil decreased DNA damage by 19% (P=0.001) and proliferation by 50% (P=0.003) and increased differentiation by 56% (P=0.039) compared to corn oil. When combined with butyrate, fish oil enhanced apoptosis 24 h post AOM injection compared to a corn oil/butyrate diet (P=0.039). There was an inverse relationship between crypt height and apoptosis in fish oil/butyrate group (r= −0.53, P=0.040). Corn oil/butyrate group showed a positive correlation between p27Kip1 expression and proliferation (r= 0.61, P=0.035). These results indicate the in vivo effect of butyrate on apoptosis and proliferation is dependent on dietary lipid source. These results demonstrate the presence of an early coordinated colonocyte response by which fish oil and butyrate protects against colon tumorigenesis.

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Expression of Cyclooxygenase-2 (COX-2) in Colorectal Adenocarcinoma: an Immunohistochemical and Histopathological Study

Cited by 18 publications

References 20 publications

Over-Expression of Cyclooxygenase-2 in Colorectal Cancer Patients

Over-Expression of Cyclooxygenase-2 in Colorectal Cancer Patients

Correlated non-nuclear COX2 and low HER2 expression confers a good prognosis in colorectal cancer

In Vivo Regulation of Colonic Cell Proliferation, Differentiation, Apoptosis, and P27Kip1 by Dietary Fish Oil and Butyrate in Rats

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