Expression of cytochrome P450 1A1 and its contribution to oxidation of a potential human carcinogen 1-phenylazo-2-naphthol (Sudan I) in human livers

Stiborová, Marie; Martínek, Václav; Rýdlová, Helena; Koblas, T.; Hodek, Petr

doi:10.1016/j.canlet.2004.07.036

Cited by 130 publications

(160 citation statements)

References 22 publications

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“…Recombinant rat CYP1A1 protein was purified to homogeneity from membranes of Escherichia coli transfected with a modified CYP1A1 cDNA [37]. Human recombinant CYP3A4 was a gift of P. Anzenbacher (Palacky University, Olomouc, Czech Republic).…”

Section: Isolation Of Cyp Enzymes and Preparation Of Antibodies Againmentioning

confidence: 99%

“…Immunoglobulin fraction was purified from pooled egg yolks using fractionation by polyethylene glycol 6000 [37,38]. …”

Section: Isolation Of Cyp Enzymes and Preparation Of Antibodies Againmentioning

confidence: 99%

“…Protein concentrations in homogenates were assessed using the bicinchoninic acid protein assay (Pierce Rockford, IL, USA) with serum albumin as a standard [18]. Samples containing 10-45 g proteins were subjected to electrophoresis on SDS/10% polyacrylamide gels [37,38]. After migration, proteins were transferred onto polyvinylidene difluoride (PVDF) (Millipore, Billerica, MA, USA) and/or nitrocellulose membranes (Bio-Rad, Hercules, CA, USA).…”

Section: Estimation Of Contents Of Cyps Cox-1 and -2 In Neuroblastommentioning

confidence: 99%

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The mechanism of cytotoxicity and DNA adduct formation by the anticancer drug ellipticine in human neuroblastoma cells

Poljaková

Eckschlager

Hraběta

et al. 2009

Biochemical Pharmacology

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Ellipticine is an antineoplastic agent, whose mode of action is based mainly on DNA intercalation, inhibition of topoisomerase II and formation of covalent DNA adducts mediated by cytochromes P450 and peroxidases. Here, the molecular mechanism of DNA-mediated ellipticine action in human neuroblastoma IMR-32, UKF-NB-3 and UKF-NB-4 cancer cell lines was investigated. Treatment of neuroblastoma cells with ellipticine resulted in apoptosis induction, which was verified by the appearance of DNA fragmentation, and in inhibition of cell growth. These effects were associated with formation of two covalent ellipticine-derived DNA adducts, identical to those formed by the cytochrome P450-and peroxidase-mediated ellipticine metabolites, 13-hydroxy-and 12-hydroxyellipticine. The expression of these enzymes at mRNA and protein levels and their ability to generate ellipticine-DNA adducts in neuroblastoma cells were proven, using the real-time polymerase chain reaction, Western blotting analyses and by analyzing ellipticine-DNA adducts in incubations of this drug with neuroblastoma S9 fractions, enzyme cofactors and DNA. The levels of DNA adducts correlated with toxicity of ellipticine to IMR-32 and UKF-NB-4 cells, but not with that to UKF-NB-3 cells. In addition, hypoxic cell culture conditions resulted in a decrease in ellipticine toxicity to IMR-32 and UKF-NB-4 cells and this correlated with lower levels of DNA adducts. Both these cell lines accumulated in S phase, suggesting that ellipticine-DNA adducts interfere with DNA replication. The results demonstrate that among the multiple modes of ellipticine antitumor action, formation of covalent DNA adducts by ellipticine is the predominant mechanism of cytotoxicity to IMR-32 and UKF-NB-4 neuroblastoma cells.

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Section: Isolation Of Cyp Enzymes and Preparation Of Antibodies Againmentioning

confidence: 99%

“…Immunoglobulin fraction was purified from pooled egg yolks using fractionation by polyethylene glycol 6000 [37,38]. …”

Section: Isolation Of Cyp Enzymes and Preparation Of Antibodies Againmentioning

confidence: 99%

Section: Estimation Of Contents Of Cyps Cox-1 and -2 In Neuroblastommentioning

confidence: 99%

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The mechanism of cytotoxicity and DNA adduct formation by the anticancer drug ellipticine in human neuroblastoma cells

Poljaková

Eckschlager

Hraběta

et al. 2009

Biochemical Pharmacology

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“…In fact, they have been associated with increased occurrence of bladder cancer in textile and leather dyers, painters, and hairdressers [2,3]. Sudan I is considered a possible human carcinogen and mutagen [4], and it is classified as a category 3 carcinogen by the International Agency for Research on Cancer (IARC) [5]. Moreover, Sudan II has been shown to cause a high frequency of bladder carcinomas in mice [6].…”

Section: Introductionmentioning

confidence: 99%

Determination of banned Sudan dyes in food samples by molecularly imprinted solid phase extraction‐high performance liquid chromatography

Baggiani¹,

Anfossi

Baravalle

et al. 2009

J of Separation Science

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Original PaperDetermination of banned Sudan dyes in food samples by molecularly imprinted solid phase extraction-high performance liquid chromatography A method for molecularly imprinted SPE of banned Sudan azo-dyes from food samples was investigated. The molecularly imprinted polymer was obtained by suspension polymerization using 1-(4-chlorophenyl)azonaphthalen-2-ol as the mimic template. The molecular recognition properties of imprinted beads were evaluated for use as a SPE sorbent, in order to develop a selective extraction protocol for the Sudan class of dyes. The optimized extraction protocol resulted in a reliable molecularly imprinted SPE (MISPE) method suitable for HPLC analysis. It was selective for the main analyte, Sudan I, and the related azo-dyes Sudan II, III, IV, Sudan Red B, and Sudan Red 7B, while the permitted azo-dyes Allura Red AC, Neococcin, and Sunset Yellow FCF were not extracted. The method was tested for Sudan I, II, III, and IV in five different food samples (hot chilli pepper, hot chilli tomato sauce, sausage, tomato sauce, and hard boiled egg yolk) at three concentration levels (15, 100, and 300 lg/g). It demonstrated itself to be insensitive to the presence of different complex matrices, precise, accurate, and with good recovery rates (85 -101%). The LOD and LOQ were satisfactory for most analytical determinations.

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“…The P450 cytochrome system (CYP450) contains a group of enzymes that are involved in steroid hormone biosynthesis and metabolic activation of carcinogens (10,11). Five different enzymes have been identified in this system, which are involved in estrogen synthesis and degradation.…”

Section: Introductionmentioning

confidence: 99%

Association of polymorphisms and haplotypes in the cytochrome P450 1B1 gene with uterine leiomyoma: A case control study

et al. 2014

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Abstract. Uterine leiomyoma (UL) is an estrogen-dependent neoplasm of the uterus and estrogen metabolizing enzymes affect its promotion and progression. The aim of the present study was to evaluate the association between four single-nucleotide polymorphisms (SNPs) of the cytochrome P450 1B1 (CYP1B1) gene and UL risk. Four SNPs of the CYP1B1 gene in 105 UL patients and 112 unrelated healthy controls were genotyped using a direct sequencing method. Haplotype analyses were performed with UNPHASED software and linkage disequilibrium (LD) was assessed by Haploview software. There were no associations between Leu432Val (rs1056836), Asp449Asp (rs1056837) and Asn453Ser (rs1800440) polymorphisms of the CYP1B1 gene and UL. Although the genotypic frequencies of the Arg368His (rs79204362) polymorphism did not differ between the two groups, the frequency of A (His) allele was significantly higher in UL females (P= 0.02). In addition, the frequency of GTAA haplotype was significantly higher in the controls and played a protective role in UL susceptibility. A strong LD between the three common SNPs (rs1056836, rs1056837 and rs1800440) in the CYP1B1 gene was observed in the population. In conclusion, a higher frequency of the CYP1B1 368His (A) allele was observed in UL females. The frequency of the GTAA haplotype was significantly higher in healthy females and this haplotype played a protective role in UL susceptibility.

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Expression of cytochrome P450 1A1 and its contribution to oxidation of a potential human carcinogen 1-phenylazo-2-naphthol (Sudan I) in human livers

Cited by 130 publications

References 22 publications

The mechanism of cytotoxicity and DNA adduct formation by the anticancer drug ellipticine in human neuroblastoma cells

The mechanism of cytotoxicity and DNA adduct formation by the anticancer drug ellipticine in human neuroblastoma cells

Determination of banned Sudan dyes in food samples by molecularly imprinted solid phase extraction‐high performance liquid chromatography

Association of polymorphisms and haplotypes in the cytochrome P450 1B1 gene with uterine leiomyoma: A case control study

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