Expression of cytokeratin‐18‐related tissue polypeptide‐specific (TPS) antigen in Wilms tumor

Rebhandl, Winfried; Handisurya, Alessandra; Memaran, Nima; Felberbauer, F. X.; Aberle, Judith H.; Paya, Kurosh; Strobl, Birgit; Horcher, Ernst

doi:10.1002/mpo.1211

Cited by 5 publications

(1 citation statement)

References 35 publications

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“…The number of CKs (40-70 kDa), at present more than 20, has been subdivided into type I (acidic, K9-K20) and type II (basic, K1-K8) based on DNA sequence homology and gene structure. CK 18 (acidic) is found in simple epithelia of normal and malignant kidney cells and of urinary bladder (Moll et al 1982;Rebhandl et al 2001). During development, CK 18 and vimentin are transiently coexpressed in vGECs (Moll et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Expression of intermediate filaments of podocytes within nephrotic syndrome glomerulopathies in children

Ostalska‐Nowicka

Zachwieja

Nowicki

et al. 2004

Histochemistry and Cell Biology

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The study attempted to define characteristics of renal podocytes in nephrotic syndrome glomerulopathies in children with and without glomerular immaturity based on the histochemical expression of cytokeratin 18 (CK 18) and vimentin. Material consisted of 29 renal biopsies performed in the Department of Pediatric Nephrology, Poznan University of Medical Sciences, between 1991 and 2000. The study group included 16 children with mesangial glomerulonephritis (MesGN) and signs of glomerular immaturity and 13 children with MesGN without signs of glomerular immaturity. The control tissue was derived from macroscopically normal renal cortex taken from kidneys resected for localised neoplasms ( n=3). In the control samples, the immunocytochemical expression of CK 18 was found only in epithelial cells of proximal and distal tubules. Vimentin was present in all podocytes, some mesangial cells and endothelium. In all cases of children with MesGN with signs of immaturity, both CK 18-positive and vimentin-positive podocytes were found. In all cases of MesGN without immaturity we revealed CK 18-negative podocytes but with distinct vimentin-positive expression. Reorganisation of cytoskeletal proteins within immature podocytes may be associated with the unfavourable clinical course of nephrotic syndrome in children. The application of antibodies against intermediate filaments may help to differentiate between mature and immature forms of MesGN.

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Section: Introductionmentioning

confidence: 99%

Expression of intermediate filaments of podocytes within nephrotic syndrome glomerulopathies in children

Ostalska‐Nowicka

Zachwieja

Nowicki

et al. 2004

Histochemistry and Cell Biology

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Molecular Targeted Therapy for Wilms’ Tumor

Geller¹,

Dome

2010

Molecularly Targeted Therapy for Childhood Cancer

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Nanoscale surface topography enhances cell adhesion and gene expression of madine darby canine kidney cells

Cheng

Zhu

Wang

et al. 2007

J Mater Sci: Mater Med

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Substrate topography is one of the key factors that influence cell behavior, such as cell attachment, adhesion, proliferation and differentiation. In the present work, nanostructures were produced on polystyrene Petri dish by polarized laser irradiation with the wavelength of 266 nm and the energy of 3.0 mJ/cm2. Cell adhesion, growth and gene expression of Madine darby canine kidney (MDCK) cells cultured on smooth and nanogrooved substrates were investigated. The results indicated that cells preferred to adhere and grow on nanogrooved substrate. The distribution of cell cycle for cells on smooth substrates was different from that on nanogrooved substrate. The percentage of G1 phase cells on nanogrooved substrate (48.6 +/- 1.4%) was lower than that on smooth substrate (57.6 +/- 4.4%), while the percentage of cells on nanogrooved substrate in S (30.2 +/- 0.5%) and G2/M (21.2 +/- 1.1%) phase was higher than those on smooth substrate (25.1 +/- 1.5% and 17.3 +/- 3.3%, respectively). Moreover, the gene expression of cyclin D1 and keratin 18, which was examined by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), was significantly enhanced by nanogrooves, with an increase of cyclin D1 mRNA by 98% and an increase of keratin 18 mRNA by 75%. In conclusion, the nanogrooved surface features on polystyrene could alter cell cycle and enhance gene expression of cyclin D1 and keratin 18 in MDCK cells, which partly explained the increased cell adhesion and growth on nanogrooved substrate.

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Expression of cytokeratin‐18‐related tissue polypeptide‐specific (TPS) antigen in Wilms tumor

Abstract: This is the first study demonstrating the synthesis and release of TPS by WTs and other paediatric renal malignancies. Considering the elevated levels of TPS in serum of these patients, a further investigation of this marker by larger clinical trials seems to be justified.

Cited by 5 publications

References 35 publications

Expression of intermediate filaments of podocytes within nephrotic syndrome glomerulopathies in children

Expression of intermediate filaments of podocytes within nephrotic syndrome glomerulopathies in children

Molecular Targeted Therapy for Wilms’ Tumor

Nanoscale surface topography enhances cell adhesion and gene expression of madine darby canine kidney cells

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